Pulp and paper mills utilizing wood materials in Indonesia mostly employ the sulphate (Kraft) process, due to its superiority. Yet it offers unfortunate weakness, among which is its contribution to the environmental pollution. Hence, it is necessary to develop appropriate technique but environmentally friendly to deal with the environmental concerns, such as biopulping process which incorporates fungi) activities to degrade lignin in wood. This experiment was mainly aimed at procuring particular fungi isolates able to do so effectively and maximally on the lignin in sengon wood (Paraserianthes falcataria (L.) Nielsen), but minimally destroying the holocellulose. The results revealed that the participation of fungi could significantly decrease lignin and extractive in sengon wood. The most substantial decrease in lignin brought about by three particular fungi isolates in decreasing order, i.e. HHB 252 (up to 20.22%), HHB 302 (20.45%) and Schizophyllum commune (21.48%). Meanwhile, the lowest decrease in holocellulose was due to the use of HHB 259 (up to 73.23%), followed by Schizophyllum commune (71.9%), and HHB 252 (71.82%). To sum-up, the best-performance fungi in the biodelignification of sengon wood was HHB 252, followed by HHB 302 and Schizophyllum commune isolates. Those three fungi isolates decreased substantially the lignin content, but concurrently brought about minimum degradation on the holocellulose, as indicated by their respective ratio of lignin to holocellulose contents, i.e. 0.282, 0.289 and 0.299.