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David S. Runtunuwu
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Social Sciences

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IDENTIFIKASI VARIETAS KENTANG “SUPERJOHN” BERDASARKAN PENANDA RAPD (Random Amplified Polymorphic DNA) Runtunuwu, David S.; Rogi, J. E.X.; Palendeng, J. H.
EUGENIA Vol 17, No 1 (2011)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.17.1.2011.7140

Abstract

ABSTRACT Identification using morphological characters has time consuming. Currently, identification using molecular markers has now been popular due to rapid, saving time and more precisely. Superjohn potato variety has been cultivated in North Sulawesi. However, the Superjohn potato variety has not been characterized using molecular markers. This research was aiming to identify “Superjohn” potato based on RAPD (Random Amplified Polymorphic DNA) markers. The research was conducted in field and laboratory. Field research was performed by taking some young leaves from “Superjohn”, Granola, and Atlantic variety from the field. Identification using molecular marker was conducted at laboratory.  Nine RAPD primers were used to identify the superjohn variety. The nine primers were OPA-1, OPA-2, OPA-3, OPA-4, OPA-5, OPA-7, OPA-9,  OPA-10, and  OPO-1.  The molecular identification revealed that “Superjohn” variety was different with Granola and Atlantic. OPA-9700 primer could be used for identification  “Superjohn” variety while OPA-101000 primer was suitable  for identification  Granola variety. Keywords:  Potatoes, variety, “Superjohn”, Granola, Atlantic, and RAPD ABSTRAK Penelitian ini dilakukan untuk mengidentikasi kentang “Superjohn” berdasarkan penanda RAPD (Random Amplified Polymorphic DNA).  Penelitian dilakukan di lapangan dan laboratorium. Penelitian lapangan dilakukan dengan mengambil beberapa daun muda dari varietas kentang “Superjohn”, Atlantik dan Granola. Kemudian  analisis DNA dilakukan di laboratorium menggunakan analisis RAPD. Berdasarkan penanda RAPD ternyata kentang “Superjohn” berbeda dari kentang Granola dan kentang Atlantik. Penanda RAPD OPA-9700 dapat digunakan untuk mengidentifikasi kentang“Superjohn” dan penanda RAPD  OPA-101000 dapat digunakan  untuk mengidentifikasi kentang Granola. Kata kunci:  Kentang,  varietas, Superjohn, Granola, Granola, RAPD
MODEL PERTUMBUHAN DAN PRODUKSI JAGUNG HIBRIDA PADA PERLAKUAN PEMBERIAN NITROGEN SERTA PEMANGKASAN TASSEL Paat, Frangky J.; Rogi, J. E.X.; Runtunuwu, David S.
EUGENIA Vol 16, No 3 (2010)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.16.3.2010.7139

Abstract

ABSTRACT This research was aimed to identify the growth and production model of hybrid maize in each treatments of nitrogen application and tassel trimming. This research was carried out in field experiment in Tountimomor Village of Kakas District in Minahasa Regency.  The result of this research showed that this model could stimulate the growth and production of maize with the total biomass of 8000 kg/ha. Optimum leaf nitrogen of 1.6 % at 49 day after cultivate. The highest leaf biomass from field measurement was reached in nitrogen fertilizing of 92 kg/ha. The highest tassel biomass from field measurement was obtained in treatment trimming which was 4 gr/ha. Keywords : Growth  Model, Production, Maize,  Nitrogen, Tassel Trimming ABSTRAK Penelitian ini bertujuan untuk mengidentifikasi pertumbuhan dan model produksi dari jagung hibrida dalam tiap-tiap perlakuan pemberian nitrogen dan pemangkasan tassel. Penelitian ini dilaksanakan di kebun percobaan di desa Tountimomor Kecamatan Kakas Kabupaten Minahasa. Hasil dari penelitian ini menunjukan bahwa model dapat menstimulasi pertumbuhan dan produksi jagung dengan jumlah biomassa 8000 kg/ha. Nitrogen daun optimum adalah 1,6% pada 49 hari setelah ditanam. Biomassa daun tertinggi didapat pada pemberian Nitrogen sebesar 92 kg/ha. Biomassa tassel tertinggi dari pengukuran lapangan didapat dalam perlakuan pemangkasa tassel sebanyak 4gr/ha. Kata kunci: Model pertumbuhan, Produksi, Jagung, Nitrogen, Pemangkasan Tassel.
PENGELOLAAN PUPUK TERPADU DALAM UPAYA MENINGKATKAN HASIL JAGUNG MANIS DAN KESUBURAN LAHAN PADA SISTEM TANAM JAJAR LEGOWO 2:1 Pamandungan, Yefta; Runtunuwu, David S.; Mamarimbing, Rinny; Najoan, Jemmy
EUGENIA Vol 22, No 1 (2016)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.22.1.2016.15105

Abstract

ABSTRACT This research aims to study the increase of the yields of sweet corn and soil fertility by applying integrated fertilizers management system in Jajar Legowo 2:1 planting system. The results of this research show that providing the 25% inorganic fertilizers plus 75% organic fertilizers as treatment has given better result for the yields of sweet corn than providing 100% inorganic fertilizers or 100% organic fertilizers as treatment. Chemical properties of the soil before and after the treatment of fertilizers, it is found out that the contents of C-Organic, N of soil, and K2O of the researched soil are categorized as low to very low. However, it is found out that the available content of P2O5 has increased by 4.29 ppm through the 25% inorganic fertilizers plus 75% organic fertilizers as treatment and has increased by 1.73 ppm through the 100% organic fertilizers as treatment. Keywords: integrated fertilizer management, sweet corn, jajar legowo
PENGARUH INTERAKSI PACLOBUTRAZOL DAN TIPE TANAM JAJAR LEGOWO PADA PRODUKSI PADI METODE TANAM SRI Christiane M. Tambajong; Edy ., Lengkong; David S. Runtunuwu
AGRI-SOSIOEKONOMI Vol. 12 No. 3A (2016)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.012 KB) | DOI: 10.35791/agrsosek.12.3A.2016.14358

Abstract

This study aims to find the effect of interaction of paclobutrazol and type Legowo Row Planting in paddy fields that use SRI cultivation method to increase rice yield, and can determine the best combination of effective and can be applied in paddy fields to increase production. The research was conducted in the Village of Sulu, Tatapaan Sub-district, South Minahasa Regency. This study uses a field experiment with split plot design in groups of two factors. As the first factor is the type of Legowo Row Planting 2: 1 and 4: 1. The second factor is the provision of paclobutrazol by spraying the plants with a dose of: 0 ppm and 600 ppm. Paclobutrazol will be given only once, that when plants in active vegetative growth period (about 30 days old plants after transplanting). Data analysis was used Two-Factor Interactions calculation. The interaction of two factors occured, if the effect of a factor changes when the change level of other factors change. The results indicated that the existence of interaction between treatment paclobutrazol and Legowo row that high interaction in the inhibition of the vegetative growth of rice crops varieties Serayu, middle interactions on the rate of plant growth, middle interactions in the treatment of paclobutrazol and Legowo row to increase the content of chlorophyll a and chlorophyll b in the leaves, the influence of high interaction of the number of productive tillers, high interaction on panicle length, high interaction to the increased weight of 1,000 grains, high interaction of the increase in the weight of dry unhusked rice per plot, and the lack of interaction to increase the number of grains per panicle. Paclobutrazol 600 ppm treatment gave a positive response on Legowo row 2: 1 for the inhibition of vegetative growth of crops to prevent crop is too high and not easily fall, increased chlorophyll a, increasing the weight of 1,000 grains and increased the weight of milled rice per plot. Paclobutrazol 600 ppm treatment gave a positive response on Legowo row 4: 1 to increase the pace of growth, increased chlorophyll b, an increase in the number of productive tiller and panicle length.
Co-Authors Christiane M. Tambajong Edy ., Lengkong Frangky J. Paat J. H. Palendeng Jemmy Najoan, Jemmy Johannes E. X. Rogi Lengkong, Edy ., Pemmy Tumewu Rinny Mamarimbing Sakti Karokaro, Sakti Tambajong, Christiane M. Yefta Pamandungan