Article Per Year (5 Year)
p-Index From 2019 - 2024
0.444
P-Index
This Author published in this journals
All Journal Indonesian Aquaculture Journal Indonesian Journal of Pharmacology and Therapy
Achmad Dinoto
Research Center for Biology, Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Neuroscience

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Antiplasmodial activity and genome mining study of marine-derived Streptomyces sp. GMY01 Ema Damayanti; Jaka Widada; Achmad Dinoto; Mustofa
Indonesian Journal of Pharmacology and Therapy Vol 2 No 2 (2021)
Publisher : Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFI) and Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (508.345 KB) | DOI: 10.22146/ijpther.1335

Abstract

High resistance to chloroquine in most malaria-endemic area in the world leads to the need for new antimalaria drugs. Marine bacterium Streptomyces is the source for potential new antimalarial molecules. This research aimed to investigate antiplasmodial activity of marine-derived of Streptomyces sp. GMY01 and to identify potential active compounds using genome mining study. In vitro antiplasmodial activity assays using flow cytometry method showed that the ethyl acetate extract of this bacterium had high antiplasmodial activity (IC50 value of 1.183 µg/mL) on Plasmodium falciparum FCR3. Genome mining analysis of whole-genome sequences using antiSMASH 6.0 beta version revealed that Streptomyces sp. GMY01 had 28 biosynthetic gene clusters (BGCs), including the genes encoding polyketide synthase, non-ribosomal peptide synthetase, terpene, lanthipeptide, bacteriocin, butyrolactone, ectoin, siderophore, and others. The known BGCs were predicted to be involved in the production of known compounds from gene clusters ranged from 5 to 100% similarity. Ongoing purification and elucidation of the structures will allow identification of the active compounds produced by marine-derived Streptomyces sp. GMY01.
ISOLATION AND IDENTIFICATION OF BACTERIA REMOVING NITRITE, NITRATE, AND AMMONIUM FROM BIOBALLS FILTER Qoriatul Ilma; Achmad Dinoto; Ninu Setianingrum; Mulyadi Mulyadi; Dwi Agustyani; Nani Radiastuti; Heddy Julistiono
Indonesian Aquaculture Journal Vol 17, No 1 (2022): (June, 2022)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.17.1.2022.13-22

Abstract

The presence of effective bacteria removing nitrite, nitrate, and ammonia in a recirculating aquaculture system (RAS) is necessary to attenuate their toxicity to fish. The research was conducted to find bacteria that can be cultured and reduce nitrite, nitrate, and ammonium. Sixteen bacterial colonies were isolated from bioballs of RAS biofilter and tested for their ability to reduce nitrite or nitrate concentrations. Using a simple indicator paper for nitrite and nitrate, four isolates that reduced nitrite and nitrate concentrations, namely K1NA3, K2NA3, CNA1, and PRO4NA1 were selected. The four isolates were then evaluated for the metabolism of nitrate, nitrite, and ammonium compounds using the spectrophotometry method. Results showed that the isolates K1NA3, CNA1, and PRO4NA1 reduced nitrite concentration but produced ammonium, whereas K1NA3 isolate was able to reduce nitrate concentration but produced both nitrite and ammonium. Experiments in reducing ammonium levels in the synthetic waste media showed the ability of four isolates to reduce ammonium levels after six days despite producing nitrite. Based on the 16S rRNA gene analysis, these isolates have a close relationship to Pseudomonas otitidis (KINA3 and K2NA3), Acinetobacter cumulans (CNA1), and Vogesella perlucida (PRO 4NA1).
Co-Authors Dwi Agustyani Ema Damayanti Heddy Julistiono Jaka Widada Mulyadi Mulyadi Mustofa Nani Radiastuti Ninu Setianingrum Qoriatul Ilma