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All Journal Buletin Peternakan VALENSI Jurnal Lahan Suboptimal AL KAUNIYAH Indonesian Journal of Agricultural Science Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Livestock and Animal Research Jurnal Biodjati Jurnal Sains Teh dan Kina Jurnal Kimia Terapan Indonesia Jurnal Mikologi Indonesia Indonesian Aquaculture Journal Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Open Access DRIVERset Makara Journal of Science
Nani Radiastuti
Program Studi Biologi, FST Universitas Islam Negeri Syarif Hidayatullah, Jakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Veterinary Other

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Alkaloid profile of endophytic Diaporthe spp. from Cinchona calisaya Radiastuti, Nani; Rahayu, Gayuh; Okane, Izumi; Hidayat, Iman; Achmadi, Suminar S.
Jurnal Penelitian Teh dan Kina Vol 18, No 1 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (420.162 KB) | DOI: 10.22302/pptk.jur.jptk.v18i1.69

Abstract

Endophytic fungi have been known as potential source of bioactive compound, similar to their host.  This study was conducted to identify endophytic Diaporthe spp. isolated from Cinchona calisaya and to explore their potential in producing alkaloids, particularly quinine. A total of 39 strains of Diaporthe spp. were identified using ITS1-5.8S-ITS2 and EF1-α gene. Seventeen strains have species name and they represent eight species of Diaporthe i.e. D. cynaroidis, D. endophytica, D. ganjae, D. gardeniae, D. litchicola, D. phaseolorum, D. pseudomangiferae, and D. rhoina. The re­maining 22 strains represents 15 groups of unidentified Diaporthe sp. High performance liquid chromatography analyses of chloroform extract of 21-days old static cultures of all fungi in potato dextrose broth showed that these strains produce totally 82 different alkaloids. Each strain producedabout 2-38 different alkaloids. Sixteen out of 39 strains (41%), i.e. one strain each of D. endophytica, D. gar­deniae,  D. phaseolorum, and  D. pseudo­mangiferae, 2 strains of D. litchicola, and 10 strains of unidentified Diaporthe were able to produce quinine. The quinine concentration ranges from 1.1 mg/l to 155.2 mg/l. By using Jaccard’s similarity index 0.44 as the cutting score, UPGMA analyses of alkaloid profile of these strains shows that these strains forms 23 clusters. Alkaloid profile clustering does not support the phylogenetic grouping. This indicates that the alkaloid profile is strain dependent. Some potential strains should be further investigated for optimization of their quinine production.
Aktivitas Antibakteri Kapang Endofit dari Tanaman Kina (Cinchona calisaya Wedd.) Zakiyah, Alfida; Radiastuti, Nani; Sumarlin, La Ode
Al-Kauniyah: Jurnal Biologi Vol 8, No 2 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.403 KB) | DOI: 10.15408/kauniyah.v8i2.2690

Abstract

Endophytic microorganisms are microorganisms that live in the tissues of plant organ and not harm its host. One of them is the endophytic fungi. Endophytic fungi could produce the same compound as the host plant. The Plants produced alkaloids quinine that could potentially inhibit the bacteria Staphylococcus aureus and Escherichia coli. This study aims to test the potential of endophytic fungi produced quinine as an antibacterial. The method used to test the antibacterial is a paper disc diffusion. Results of the analysis of data using one-way ANOVA showed that there are significant differences zona between the diametre of inhibitory of the endophytic fungi extract. Endophytic fungi M16 and M33 produced the greatest inhibition zone against Staphylococcus aureus and Escherichia coli 14.9 mm and 9.2 mm respectively.
DIVERSITY OF EXTRACELLULAR ENZYMES PRODUCED BY ENDOPHYTIC FUNGUS ORIGINATED FROM Centella asiatica (L.) Urban Susilowati, Dwi Ningsih; Setiyani, Alfi Dwi; Radiastuti, Nani; Sofiana, Indah; Suryadi, Yadi
Jurnal Penelitian Tanaman Industri Vol 26, No 2 (2020): December, 2020
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v26n2.2020.78-91

Abstract

Asiatic Pennyworth (Centella asiatica) is a medicinal plant known to be symbiotic with various types of endophytic fungi. There are extensively studied as a source of new bioactive compounds, including extracellular enzymes. This study aimed to characterize enzymes produced by 40 endophytic fungi from C. asiatica. This research was conducted at the Microbiology Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development Bogor and the Microbiology Laboratory, PLT UIN Syarif Hidayatullah Jakarta in February to April 2019. Seven enzymes screened were asparaginase, amylase, cellulase, pectinase, protease, glucanase, and laccase on Potato Dextrose Agar enriched with a specific substrate. The results showed that the number and type of enzymes produced by the fungi varied.  Phanerochaete chrysosporium MB02, Fusarium falciforme MB07, Trichaptum sp.MB11, Fusariumkeratoplasticum MB12, Penicillium capsulatum MB15, Phomamultirostrata MB16, Fusarium oxysporum MB17, and Mycochaetophora gentianae MB21 produced the highest enzyme number, i.e., six types of enzymes.  Colletotrichum tabaci MB14 produced the highest index value for asparaginase (index 2.65), Fusarium keratoplasticum MB12, Colletotrichum tabaci MB14, and Phoma multirostrata MB16 for amylase (index 2.00); Peroneutypa scoparia MM10 for cellulase (index 4.10); Colletotrichum karstii MM02 for pectinase (index4.12); C. tabaci MB14 for protease (index 4.37); Acrocalymma vagum MB04 for glucanase (index 1.68); and Fusarium solani MM03 for laccase (index 0.22). Colletotrichum tabaci MB14 was superior because it produced the highest of 3 enzymes (asparaginase, amylase, and protease).  Further study is required to find optimal conditions for each enzyme production for industrial purposes.Keywords: Asiatic Pennyworth, extracellular enzyme, in vitro production Abstrak KERAGAMAN ENZIM EKSTRASELULER DIHASILKAN OLEH JAMUR ENDOFIT ASAL Centella asiatica (L.) UrbanTanaman Pegagan (Centella asiatica) adalah tanaman obat yang dikenal bersimbiosis dengan berbagai jenis jamur endofit. Jamur endofit dipelajari secara ekstensif sebagai sumber senyawa bioaktif baru, termasuk enzim ekstraseluler. Enzim asparaginase, amilase, selulase, pektinase, protease, glukanase, dan lakase digunakan dalam industri. Penelitian ini bertujuan untuk mengkarakterisasi beberapa produksi enzim dari 40 jamur endofit dari C. asiatica. Penelitian dilakukan di Laboratorium Mikrobiologi, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian Bogor dan Laboratorium Mikrobiologi, PLT UIN Syarif Hidayatullah Jakarta pada bulan Februari hingga April 2019. Skrining enzim asparaginase, amilase, selulase, pektinase, protease, glukanase, dan lakase dilakukan pada medium Potato Dextrose Agar yang diperkaya dengan substrat tertentu. Hasil penelitian menunjukkan bahwa jumlah dan jenis enzim yang dihasilkan oleh jamur bervariasi. Phanerochaete chrysosporium MB02, Fusarium falciforme MB07, Trichaptum sp. MB11, Fusarium keratoplasticum MB12, Penicillium capsulatum MB15, Phoma multirostrata MB16, Fusarium oxysporum MB17, dan Mycochaetophora gentianae MB21 menghasilkan jumlah enzim tertinggi (6 jenis enzim). Berdasarkan enzim yang diproduksi (nilai indeks), Colletotrichum tabaci MB14 menghasilkan asparaginase tertinggi (indeks 2,65), Fusarium keratoplasticum MB12, Colletotrichum tabaci MB14, dan Phoma multirostrata MB16 untuk amilase (indeks 2,00); Peroneutypa scoparia MM10 untuk selulase (indeks 4.10); Colletotrichum karstii MM02 untuk pektinase (indeks 4.12); C. tabaci MB14 untuk protease (indeks 4.37); Acrocalymma vagum MB04 untuk glukanase (indeks 1,68); dan Fusarium solani MM03 untuk lakase (indeks 0,22). Colletotrichum tabaci MB14 merupakan isolat yang unggul penghasil 3 jenis enzim tertinggi (asparaginase, amilase, dan protease).  Perlu dilakukan penelitian lebih lanjut untuk menganalisis secara kuantitatif produksi enzim ekstraseluler yang dihasilkan dan prospeknya untuk keperluan industri.Kata kunci:  Enzim ekstraseluler, pegagan, produksi in vitro 
DIVERSITY OF EXTRACELLULAR ENZYMES PRODUCED BY ENDOPHYTIC FUNGUS ORIGINATED FROM Centella asiatica (L.) Urban Susilowati, Dwi Ningsih; Setiyani, Alfi Dwi; Radiastuti, Nani; Sofiana, Indah; Suryadi, Yadi
Jurnal Penelitian Tanaman Industri Vol 26, No 2 (2020): December, 2020
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v26n2.2020.78-91

Abstract

Asiatic Pennyworth (Centella asiatica) is a medicinal plant known to be symbiotic with various types of endophytic fungi. There are extensively studied as a source of new bioactive compounds, including extracellular enzymes. This study aimed to characterize enzymes produced by 40 endophytic fungi from C. asiatica. This research was conducted at the Microbiology Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development Bogor and the Microbiology Laboratory, PLT UIN Syarif Hidayatullah Jakarta in February to April 2019. Seven enzymes screened were asparaginase, amylase, cellulase, pectinase, protease, glucanase, and laccase on Potato Dextrose Agar enriched with a specific substrate. The results showed that the number and type of enzymes produced by the fungi varied.  Phanerochaete chrysosporium MB02, Fusarium falciforme MB07, Trichaptum sp.MB11, Fusariumkeratoplasticum MB12, Penicillium capsulatum MB15, Phomamultirostrata MB16, Fusarium oxysporum MB17, and Mycochaetophora gentianae MB21 produced the highest enzyme number, i.e., six types of enzymes.  Colletotrichum tabaci MB14 produced the highest index value for asparaginase (index 2.65), Fusarium keratoplasticum MB12, Colletotrichum tabaci MB14, and Phoma multirostrata MB16 for amylase (index 2.00); Peroneutypa scoparia MM10 for cellulase (index 4.10); Colletotrichum karstii MM02 for pectinase (index4.12); C. tabaci MB14 for protease (index 4.37); Acrocalymma vagum MB04 for glucanase (index 1.68); and Fusarium solani MM03 for laccase (index 0.22). Colletotrichum tabaci MB14 was superior because it produced the highest of 3 enzymes (asparaginase, amylase, and protease).  Further study is required to find optimal conditions for each enzyme production for industrial purposes.Keywords: Asiatic Pennyworth, extracellular enzyme, in vitro production Abstrak KERAGAMAN ENZIM EKSTRASELULER DIHASILKAN OLEH JAMUR ENDOFIT ASAL Centella asiatica (L.) UrbanTanaman Pegagan (Centella asiatica) adalah tanaman obat yang dikenal bersimbiosis dengan berbagai jenis jamur endofit. Jamur endofit dipelajari secara ekstensif sebagai sumber senyawa bioaktif baru, termasuk enzim ekstraseluler. Enzim asparaginase, amilase, selulase, pektinase, protease, glukanase, dan lakase digunakan dalam industri. Penelitian ini bertujuan untuk mengkarakterisasi beberapa produksi enzim dari 40 jamur endofit dari C. asiatica. Penelitian dilakukan di Laboratorium Mikrobiologi, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian Bogor dan Laboratorium Mikrobiologi, PLT UIN Syarif Hidayatullah Jakarta pada bulan Februari hingga April 2019. Skrining enzim asparaginase, amilase, selulase, pektinase, protease, glukanase, dan lakase dilakukan pada medium Potato Dextrose Agar yang diperkaya dengan substrat tertentu. Hasil penelitian menunjukkan bahwa jumlah dan jenis enzim yang dihasilkan oleh jamur bervariasi. Phanerochaete chrysosporium MB02, Fusarium falciforme MB07, Trichaptum sp. MB11, Fusarium keratoplasticum MB12, Penicillium capsulatum MB15, Phoma multirostrata MB16, Fusarium oxysporum MB17, dan Mycochaetophora gentianae MB21 menghasilkan jumlah enzim tertinggi (6 jenis enzim). Berdasarkan enzim yang diproduksi (nilai indeks), Colletotrichum tabaci MB14 menghasilkan asparaginase tertinggi (indeks 2,65), Fusarium keratoplasticum MB12, Colletotrichum tabaci MB14, dan Phoma multirostrata MB16 untuk amilase (indeks 2,00); Peroneutypa scoparia MM10 untuk selulase (indeks 4.10); Colletotrichum karstii MM02 untuk pektinase (indeks 4.12); C. tabaci MB14 untuk protease (indeks 4.37); Acrocalymma vagum MB04 untuk glukanase (indeks 1,68); dan Fusarium solani MM03 untuk lakase (indeks 0,22). Colletotrichum tabaci MB14 merupakan isolat yang unggul penghasil 3 jenis enzim tertinggi (asparaginase, amilase, dan protease).  Perlu dilakukan penelitian lebih lanjut untuk menganalisis secara kuantitatif produksi enzim ekstraseluler yang dihasilkan dan prospeknya untuk keperluan industri.Kata kunci:  Enzim ekstraseluler, pegagan, produksi in vitro 
Potency of Papaya Seed Powder (Carica papaya L.) as Chicken Meat and Shrimp Preservative Nani Radiastuti; Reno Fitri; Gusniar Berty
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.9267

Abstract

 Chicken meat and shrimp are perishable food materials. Giving natural preservatives is one way to extend the storage time of food. This study aimed to know the potential of papaya seed powder varieties of ‘Bangkok’ and ‘California’ as preservatives. The sample consisted of two varieties of papaya seed powder with concentrations of 2,4,6 and 8 g. Storage time for chicken meat and shrimp was the third days. Parameters measured were total bacterial colonies, pH va-lues, and organoleptic characteristics (color, aroma and texture) of fresh chicken meat and shrimp. The result showed papaya seed pow-der influences the quality of chicken meat and shrimp. fresh chicken meat and shrimp with papaya seed powder varieties ‘Bangkok’ and ‘California’ had a lower total bacterial colony as much as 9 x 105 until 1x106 compared to control which were 4.2 x 107 - 1.87 x 107. Organoleptic result of chicken meat and shrimp with the treatment of papaya seed powder and stored for three days continue to decrease in term of color, aroma and texture. In chicken meat, the decrease in bacterial growth was still within the limits of microorganism con-tamination quality in animal-based food. However, the same con-centrations of papaya seed powder in the shrimp have not been able to reduce bacterial growth until the limit permitted by SNI. 
Karakterisasi Senyawa Aktif Antibakteri Ekstrak Air Bunga Kecombrang (Etlingera elatior) Sebagai Bahan Pangan Fungsional Dede Sukandar; Nani Radiastuti; Ira Jayanegara; Adeng Hudaya
Jurnal Kimia Valensi Jurnal Valensi Volume 2, No.1, November 2010
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (596.662 KB) | DOI: 10.15408/jkv.v2i1.232

Abstract

Telah dilaporkan penelitian mengenai aktivitas antimikroba ekstrak air bunga kecombrang(Etlingera elatior). Penelitian ini bertujuan memberikan bukti ilmiah keunggulan tanamankecombrang sebagai bahan pangan fungsional.. Pengujian aktivitas antibakteri dilakukanmenggunakan metode difusi cakram dan analisa komponen kimia dengan kromatografi GCMS.Ekstrak air bunga kecombrang bersifat antibakteri terhadap E. Coli (zona hambat 4,8mm/konsentrasi 60%) dan S. Aureus (zona hambat 6,87 mm, konsentrasi 20%). Ekstrak air bungakecombrang memiliki komponen utama1-dodekanol (tR=11,60, area=11,73, kemiripan 95 %), 3-metil-1-okso-2-buten 1-(21,41, 51-trihidroksi fenil) (tR=13,02, area=3,17 kemiripan 57 %) dan 1-tetradekena (tR= 13,26, area=6,03, kemiripan 98 %.
Antioxidant Activity From Water Extract Of Kecombrang Flower (Etlingera elatior) Leading To Jelly Candy Formulation Dede Sukandar; Nani Radiastuti; Anna Muawanah; Adeng Hudaya
Jurnal Kimia Valensi Jurnal Valensi Volume 2, No.2, Mei 2011
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v2i2.203

Abstract

A research on antioxidant activity of kecombrang flower ( Etlingera Elatior) water extract leading tojelly candy formulation was conducted. This experiment aims to elucidate the potential usage ofkecombrang flower as a functional food ingredient. Antioxidant activity of the kecombrang flowerwater extract was determined using Diphenyl Picryl Hidrazyl ( DPPH) method. In order to elucidatethe responsible compound for antioxidant activity, GCMS analysis was undertaken. Water extract ofkecombrang flower have antioxidant activity ( IC50=61,6497 ppm) and based on GCMS analysis itcontained 1-dodekanol ( tR=11,60, area=11,73, similarity at 95 %), 3-metil-1-oxo-2-buten 1-(21,41,51-trihidroxyl phenil) ( tR=13,02, area=3,17, similarity at 57 %) and 1-tetradecene ( tR= 13,26,area=6,03, similarity at 98 %). Formulation of jelly candy using kecombrang flower was designedand based on organoleptic test, C formula ( pink, sample code 763) was the optimum jelly pepermintformula with average score of 3,52.
Penggunaan Bunga Kecombrang (Etlingera Elatior) Dalam Proses Formulasi Permen Jelly Anna Muawanah; Ira Djajanegara; A Sa’duddin; Dede Sukandar; Nani radiastuti
Jurnal Kimia Valensi Jurnal Valensi Volume 2, No.4, Mei 2012
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v2i4.270

Abstract

Penelitian penggunaan bunga kecombrang dalam formulasi permen jelly telah dilakukan. Tujuan daripenelitian ini adalah mengetahui formulasi yang paling disukai berdasarkan uji organoleptik danspesifikasinya berdasarkan standar permen jelly dalam SNI 3547.2-2008. Penelitian ini terdiri dari duatahap yaitu pertama penentuan formulasi permen jelly dan kedua analisis produk meliputi sifat fisik,sifat kimia, aktivitas antioksidan, cemaran logam dan total mikroba. Sediaan bunga kecombrang yangdigunakan dalam formulasi permen jelly adalah ekstrak air dan manisan bunga kecombrang. Hasilpenelitian menunjukan bahwa formula kode M2 merupakan produk yang paling disukai oleh panelisberdasarkan uji organoleptik.Spesifikasi permen jellytersebut adalah kekerasan 130,43 g force;kekenyalan 10; pH 3,84; kadar air 4,92% (b/b); kadar abu 0,14% (b/b); kadar protein 7,34% (b/b);kadar lemak 0,15% (b/b); kadar gula pereduksi 6,18% (b/b); aktivitas antioksidan (IC50) 161,82μg/mL; logam Pb 0,52 mg/L dan total mikroba permen jelly sampai akhir penyimpanan pada hari ke-4masih dibawah 5x104 koloni/gram yaitu 8,19x103 koloni/gram produk. Berdasarkan standar permenjelly, spesifikasi permen jellyyang paling disukai telah memenuhi standar yang ditetapkan dalam SNI3547.2-2008.
POTENTIAL OF ENDOPHYTIC FUNGI DERIVING FROM ASIATIC PENNYWORTH TO PRODUCE ANTIOXIDANTS Dwi Ningsih Susilowati; Nani Radiastuti; Nurhasni Nurhasni; Lisca Puji Rustanti; Ika Roostika Tambunan
Indonesian Journal of Agricultural Science Vol 22, No 2 (2021): DECEMBER 2021
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v22n2.2021.p58-65

Abstract

Asiatic pennyworth is a medicinal plant that contains triterpenoids, saponin, flavonoids, and tannins which possess antioxidants. Endophytic fungi from the plant could produce a similar compound; therefore, antioxidants could be made in the laboratory if the fungi are isolated. This study aimed to evaluate the potential of endophytic fungi isolated from Asiatic pennyworth to produce antioxidants. The study used 34 endophytic fungal isolates from Asiatic pennyworth accessions of Malaysia (17 isolates) and Bengkulu, Indonesia (17 isolates) collected by the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. The fungi were propagated in a potato dextrose broth medium, then mycelia mats and filtrates were separated and then freeze-dried. The antioxidant activities were first tested with 1,1-diphenyl-2- picrylhydrazyl (DPPH) using thin layer chromatography (TLC), then UV-Vis spectrophotometry λ517 nm with five variations of concentration. Results showed all 34 fungal isolates have antioxidant activities based on a yellowish-white color change after applying 0.002% DPPH solution of the TLC method and IC50 value of the UV-Vis spectrophotometer. The highest antioxidant activity was shown by Aspergillus austroafricanus MB 1 (IC50 = 12.08 ppm) from Bengkulu accession and A. oryzae MM 13 (IC50 = 10.29 ppm) from Malaysia accession. A. austroafricanus MB 1 produced more antioxidant compounds (seven) than A. oryzae MM 13 (six). The antioxidant compounds produced by both endophytic fungi included in the group of flavonoids, fatty acids, and carboxylic acids. The research implies that A. austroafricanus MB 1 and A. oryzae MM 13 could be further developed as sources of antioxidants.
The Potential of Nutmeg’s Microbes (Myristica fragrans Houtt.) as Antagonistic Agents against Rigidoporus microporus Dwi N Susilowati; Sri Rahayuningsih; Indah Sofiana; Nani Radiastuti
Jurnal Lahan Suboptimal : Journal of Suboptimal Lands Vol. 10 No. 1 (2021): JLSO
Publisher : Research Center for Sub-optimal Lands (PUR-PLSO), Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/JLSO.10.1.2021.529

Abstract

This study aimed to obtain yeast and bacteria from Myristica fragrans Houtt., which have the potential to produce chitinase enzymes with antagonistic ability against Rigidoporus microporous. Both microorganisms were extracted from the leaves and fruit of nutmeg. A total of 35 yeast and 29 bacterial isolates were obtained, with different morphological characters. The chitinolytic test was carried out qualitatively, and the parameters observed include the clear zones around the colony. A total of 4 bacterial isolates produced chitinase enzymes (BP 1.2.1, BP 2.1.1, EPBj II.K1, and EPBj II. K2) with a chitinolytic index of 3.92, 5.38, 2.00, and 1.66, respectively. Yeast isolates were negative for chitinase enzymes. The difference in index value indicated a variation in enzyme activity. The antagonist test was carried out using a dual culture method. A total of 1 yeast and 14 bacterial isolates inhibited the growth of R. microporous, and each has a different inhibitory zone. Based on the percentage of inhibition value, the highest percentage occurred in P.K1(41.1%), P. K2 (50%), dan EPBj II. K6 (42.2%). The antagonist test indicator includes the formation of inhibitory zones on the medium. Hemolysis test showed that yeast and bacteria are not able to break down blood cells in the medium. The molecular identification showed that P. K1 and P. K2 isolates were classified as Bacillus subtillis and EPBj II. K6 were identified as Bacillus aerius with 100% sequence homology and 99% bootstrap value respectively. These findings provided information about potential microbes that control white root fungus.       
Co-Authors A Sa’duddin Achmad Dinoto Adeng Hudaya Aina Nadila Alfida Zakiyah, Alfida Amelia Rakhmaniar Anna Muawanah Dede Sukandar Dianna Rossyta Pratiwi Dwi Agustyani Dwi N Susilowati Dwi Ningsih Susilowati Elpawati, Elpawati Endang Tri Margawati Endang Tri Margawati F Wijayanti Gayuh Rahayu Gusniar Berty Hajar Indra Wardhana Heddy Julistiono Ika Roostika Ika Roostika Tambunan Iman Hidayat Indah Sofiana Indri Garnasih Ira Djajanegara Ira Djajanegara Ira Jayanegara Izumi Okane, Izumi Khoeriyah Khoeriyah La Ode Sumarlin Lisca Puji Rustanti Mulyadi Mulyadi Ninu Setianingrum Nurhasni Nurhasni Qoriatul Ilma R Fathoni Reno Fitri Rina Ningtiyas Sena Yunia Saputri Setiyani, Alfi Dwi Slamet Diah Volkandari Slamet Diah Volkandari Sofiana, Indah Sri Rahayuningsih Suminar S. Achmadi Suryadi, Yadi Susilowati, Dwi Ningsih