Garuda - Garba Rujukan Digital

Narita Syawalia Ridzwan

Research Institute for Fish Breeding, Sukamandi

Author-ID : 3672337

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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ASSOCIATION OF MICROSATELLITE GENETIC DIVERSITY WITH GROWTH RELATED TRAITS IN THE BASE POPULATION OF AFRICAN CATFISH, Clarias gariepinus, BREEDING PROGRAM Imron Imron; Bambang Iswanto; Narita Syawalia Ridzwan; Rommy Suprapto; Huria Marnis
Indonesian Aquaculture Journal Vol 10, No 1 (2015): (June 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Genetic diversity at molecular level has been assumed to correlate with fitness related traits. However, accumulating evidences showed that the nature of that correlation has been variable. This study was aimed to explore the nature and possible mechanisms underlying that correlation by focusing on growth related traits in African catfish, Clarias gariepinus using microsatellite molecular markers. Fifty individual African catfish of 110 days-old were sampled and subjected to both morphological and molecular analyses. The standard length, total length and body weight as well as allelic scores of six microsatellite loci were measured on each individual. Indices of microsatellite diversity, namely individual multilocus heterozygosity (MLH) and mean microsatellite allelic distance (mean d2) for individual level, and mean observed heterozygosity (Ho) and single-locus heterozygosity (ho) for group level, were correlated to those traits using Pearson correlation coefficient (r). The Hardy Weinberg and linkage disequilibrium were carried out to explore the possible mechanisms underlying correlation. The results showed that at individual level the MLH and mean d2 were weakly correlated with standard length, (r=0.25, p<0.05) and (r=0.24, p<0.05), respectively. At group level, Ho was correlated with both standard and total length (r=0.99, p<0.05) while ho identified two loci, Cga03 and Cga06 significantly contributed to the correlation. Combining all relevant information, present study identified associative overdominance, both local effect and general effect hypotheses might responsible for the observed correlations.

EFFECT OF CALCIUM SUPPLEMENTATION ON GIANT FRESHWATER PRAWN (Macrobrachium rosenbergii) MOULTING AND EGG QUALITY Ikhsan Khasani; Narita Syawalia Ridzwan; Clive Jones
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

A breeding program for giant freshwater prawn has been developed in Indonesia to supply good quality prawn seed to industry. To achieve the goal of the program, optimum conditions of both environment and nutrition must be provided. Calcium is the main compound of the prawn carapace, influencing moulting processes, especially during the premating moult. The effect of calcium supplementation in the prawn feed on moulting process and egg quality of giant freshwater prawn, Macrobrachium rosenbergii, was investigated. Experimental units consisted of 300 L plastic tanks stocked with 4 adult prawns comprising 3 female and 1 male. A standard prawn feed containing 30.39% crude protein; 0.82% Ca and 0.47% P was used as the experimental diet. A complete randomized design was‘applied in the study with 5 dosage levels of dicalcium phosphate i.e. 0%, 2%, 4%, 6%, and 8% with 4 replicates. Prawns were reared over 45 days. There were significant differences (P<0.05) in premoult, moult, and egg hatching rate, while no significant differences in intermoult or fecundity were observed (P>0.05). Egg hatching rate increased liniearly from 26.5%±9.9% to 50.8%±10.3% as calcium dosage increased from 0% to 8%. The number of eggs per spawn was not significantly different (P>0.05), ranging from 40,096 to 46,131 for females weighing 30.19 to 32.94 g. The results of this study suggest that giant freshwater prawns require dietary calcium supplementation to support moulting process and egg quality when reared in soft water.

THE DYNAMICS OF GENETIC VARIABILITY IN THREE GENERATIONS OF MASS SELECTION FOR FAST GROWTH IN AFRICAN CATFISH, Clarias gariepinus ASSESSED BY MICROSATELLITE MARKERS Imron Imron; Bambang Iswanto; Huria Marnis; Rommy Suprapto; Narita Syawalia Ridzwan
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Selective breeding aiming at improving the performance of economically important traits acts by exploiting population’s phenotypic variance. Due to the relationship between phenotype and genotype, selection on phenotype may also affect the profile of genotype. This study was aimed to monitor the impact of three generations of mass selection for fast growth in African catfish, Clarias gariepinus, on genetic variability, assessed by microsatellite. A total of 350 fish representing four populations, namely a composite base population (G-0), selected lines of the first generation (G-1) to the third generation (G-3), were sampled. The samples were screened for their genetic diversity using five microsatellite loci1 namely cga01, cga02, cga03, cga05, and cga09. Several genetic parameters including number of allele (A), allelic richness (AR), observed (Ho) and expected (He) heterozygosity, and fixation index (Fis) were evaluated. The results showed that there was a slight increase in the value of diversity indices in the G-1 relative to the G-0 and to the other two generations. Among these parameters, the number of allele seemed to be the most sensitive parameter in detecting genetic changes. All populations experienced heterozygote deficit and positive fixation index indicating the phenomena of inbreeding. Overall, selection for growth for three generations in African catfish breeding program resulted in significant genetic differentiation between populations. Further, the level of genetic differentiation seemed to accumulate along with the number of generaton in breeding program. However, selection did not result in a decline in genetic diversity within population. A relatively short period of the program, along with the use a high number of broodstock (mating pairs) to produce each generation seems to be able to maintain the stability of genetic diversity of the population.

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