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Raden Roro Sri Pudji Sinarni Dewi

Research Institute for Fish Breeding, Sukamandi

Author-ID : 3672510

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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NUTRITIVE COMPOSITION OF RED TILAPIA REARED IN FRESHWATER AND BRACKISHWATER Raden Roro Sri Pudji Sinarni Dewi; Priadi Setyawan; Evi Tahapari; Adam Robisalmi; Nunuk Listiyowati
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The aim of this research was to investigate the nutritive composition (especially fatty acids) in red tilapia that was reared in freshwater and brackishwater. The fatty acid contents were determined by gas chromatography. The fatty acids profile were -3 (linolenic acid, eicosapentaenoic acid/EPA, docosahexaenoic acid/DHA), -6 (linoleic acid, arachidonic acid/AA), and -9 (oleic acid). Red tilapia samples were obtained from Research Institute for Fish Breeding, Sukamandi, West Java (freshwater ponds) and Congot, Yogyakarta (brackishwater ponds; salinity 20 ppt). In this research, red tilapia reared in different ecosystems showed different fatty acid profiles. Red tilapia inhabiting brackishwater ecosystem has EPA (0.26±0.05%), DHA (3.42±0.26%), and linoleic acid (17.20±0.56%) content higher than freshwater ecosystem (EPA = 0%; DHA = 0.67±0.44%; linoleic acid = 9.08±4.76%), except for linolenic acid (0.30±0.15% vs 0.25±0.10%), arachidonic acid (0.77±0.39% vs 0.93±0.13%) and oleic acid (38.67±2.58% vs 37.44±0.74%). The ratio of -6/-3 in red tilapia inhabiting freshwater ecosystem was about 11/1. The culture tilapia in brackishwater ecosystem decrease -6/-3 ratio (4.5:1). So that for human health, it will be better to consume brackishwater red tilapia than freshwater red tilapia.

DETECTION, TRANSMISSION, AND EXPRESSION cDNA GROWTH HORMONE GENE (PhGH) OF STRIPPED CATFISH IN F-1 TRANSGENIC AFRICAN CATFISH Huria Marnis; Bambang Iswanto; Rommy Suprapto; Imron Imron; Raden Roro Sri Pudji Sinarni Dewi
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

In previous study, the fast growth transgenic founder of African catfish was produced harboring a growth hormone (GH) gene construct containing a stripped catfish growth hormone (PhGH) cDNA. This study was conducted to investigate transgene (PhGH) transmission and expression in F-1 transgenic African catfish. The transgenic founders (female) were crossed with non-transgenic (male) to produce heterozygous F-1 progeny. PhGH gene was detected in the embryo, larvae, and seed of the transgenic F-1 using PCR method. Expression levels of transgene in embryo and larvae were analyzed using real-time quantitative PCR (qPCR) method. The transgene was detected in embryo, larvae and seed of F-1 transgenic African catfish. Founder could transmit PhGH gene to transgenic F-1 lines in ranged 36% to 48%. Expression level of Phgh gene in embryo was higher than that of the larvae; whereas in the embryo was 1.5 x 105 - 5.2 x 105 copies or 0.49-9.82 fold, while in the larvae was 1.1 x 105 - 2.5 x 105copies or 0.19-5.80 fold.

THE IMPACTS OF FASTING PERIODS ON FOOD INTAKE, GROWTH RATE, COMPENSATORY GROWTH, AND EFFICIENCY OF FEED UTILIZATION IN BLUE TILAPIA (Oreochromis aureus) REARED IN BRACKISH WATER PONDS Priadi Setyawan; Adam Robisalmi; Nunuk Listiyowati; Raden Roro Sri Pudji Sinarni Dewi; Imron Imron
Indonesian Aquaculture Journal Vol 7, No 2 (2012): (December 2012)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Blue tilapia (Oreochromis aureus) has known as euryhaline species refers to wide range of salinity tolerance. The pure population or hybrid with other strain of tilapia can be a good candidate in marginal coastal land use. Tilapia is the most important aquaculture species in Indonesia. Refers to FAO 2010, Indonesia is the third biggest of tilapia production after China and Egypt. One of the main problems in aquaculture is their feed as the major cost in fish farming. Various techniques had carried out to reduce of feed cost such as improving fish quality, sex reversal and feed management. This research aimed to determine of compensatory growth in tilapia as one technique in feed management. Fry obtained from natural spawning in freshwater pond. Acclimatization in 20 ppt made in aquarium for 4 days after one month reared in hapas. Fish reared in 2 m x 1 m hapas with five treatments and three replications. This research had conducted at brackish water pond in Yogyakarta. The treatments is A: one day fasting and six days feeding (1/6), B: 2/5, C: 3/4, D: 4/3 and E is control. Results showed that the biggest of average weight gain is treatment of E (68.36 g) followed by A (66.38 g), B (62.44 g), C (43.56 g), and D (27.30 g) respectively. One-way ANOVAs analysis with 95% of interval confidences continued with Tukey’s Pairwaise comparison showed that nothing significant different between E, A, and B. The biggest of daily growth rate and specific growth rate is E (0.75 g/day and 4.68 %bw/day) followed by A (0.73 g/day and 4.65 %bw/day); B (0.69 g/day and 4.58 %bw/day); C (0.48 g/day and 4.18% bw/day) and D (0.29 g/day and 3.65% bw/day) respectively. Survival rate of A is 73.00% followed by E. 72.00%, C. 71.00%, D. 69.69%, and B. 67.00% respectively. Feed conversion ratio of D is 0.83 followed by C (0.87), B (0.98), A (1.16), and E (1.41). Food efficiency ratio of D is 127.06% followed by C (118.75%), B (106.09%), A (88.87%), and E (73.38%). These results indicate that fasting of one day and two days has no significant effect on fish growth. Treatment A and B is the better treatment for fish culture refers to the better value of FCR, FER, and total weight gain.

GONAD REMATURATION ON Pangasionodon hypophthalmus FEMALE THROUGH INJECTION OF PREGNANT MARE SERUM GONADOTROPIN AND HUMAN CHORIONIC GONADOTROPIN Evi Tahapari; Jadmiko Darmawan; Raden Roro Sri Pudji Sinarni Dewi; Wahyu Pamungkas; Huria Marnis
Indonesian Aquaculture Journal Vol 9, No 1 (2014): (June 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The success of spawning is influenced by internal and external factors. One of the factors that affect the var iabi li ty of Pangasianodon hypophthalmus female reproductive is the change of seasons that cause disrupted continuity of the seed availability, especially in the dry season. In the present study, combination of PMSG (pregnant mare serum gonadotropin) + HCG (hormone chorionic gonadotropin) hormone injections was done to induce gonad development. The treatments in this study were without hormone injections as control (A), injection of PMSG 10 IU/kg + HCG 10 IU/kg (B), and injection of PMSG 20 IU/kg HCG + 10 IU/kg (C). Injections were conducted at intervals of two weeks as many as six times. The results showed that gonad maturation generally occurs 2-4 weeks after estradiol-17 peak. PMSG + HCG hormone injections gave a significant effect on increasing the quantity and quality of eggs production. The fecundity in the A, B, C treatments, were 233,700±220,676; 300,305±24,581 and 488,433±142,228; respectively. Number of larvae produced from the A, B, C treatments, were 156,979±170,838; 229,997±18,081 and 362,713±101,850; respectively. Combination of PMSG 20 IU/kg + HCG 10 IU/kg hormone injection gave the best result on fecundity and the number of larvae production.

DISTRIBUTION AND EXPRESSION OF STRIPED CATFISH (Pangasionodon hypophtalmus) GROWTH HORMONE GENE (PhGH) IN THE ORGAN OF AFRICAN CATFISH (Clarias gariepinus) TRANSGENIC FOUNDER Huria Marnis; Raden Roro Sri Pudji Sinarni Dewi; Imron Imron; Bambang Iswanto
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Faster growing African catfish can be produced by transgenesis. This study was conducted to investigate the distribution and expression of growth hormone gene (PhGH) in various organs of the transgenic African catfish (Clarias gariepinus) founder (F0). Transgene was detected using the PCR method in various organs, namely pituitary, brain, liver, heart, spleen, kidney, intestine, stomach, muscle, caudal fin, gill and eye. Transgene expression levels were analyzed using the method of reverse transcriptase-polymerase chain reaction (RT-PCR), -actin gene used as internal controls. The results showed that the PhGH was detected and expressed in all organs of the transgenic African catfish founder. The high level of PhGH expression was found in the liver, pituitary, intestine and brain; smaller amounts were detectable in muscle, spleen, kidneys, heart, and stomach, caudal fin, gill and eyes, range from 0.02-0.75 PhGH/-actin mRNA. The expression levels of PhGH had positive correlation with tissue and body size (P<0.05).

Co-Authors Adam Robisalmi Bambang Iswanto Evi Tahapari Huria Marnis Imron Imron Jadmiko Darmawan Nunuk Listiyowati Priadi Setyawan Rommy Suprapto Wahyu Pamungkas

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