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Rosmiati Rosmiati
Research and Development Institute for Coastal Aquaculture, Maros
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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IN VITRO GROWTH RATE OF Kappaphycus alvarezii MICROPROPAGULE AND EMBRYO BY ENRICHMENT MEDIUM WITH SEAWEED EXTRACT Emma Suryati; Rosmiati Rosmiati; Andi Parenrengi; Andi Tenriulo
Indonesian Aquaculture Journal Vol 10, No 1 (2015): (June 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.68 KB) | DOI: 10.15578/iaj.10.1.2015.13-17

Abstract

The development of micropropagule and embryo of seaweed depend on nutrient and fertilizer used. Seaweed has been reported contain hormone regulators such as auxine, cytokinine, gibbereline, and various minerals applied in stimulating the growth ocra plant and wheat culture. The objectives of this study were to determine the potential of Kappaphycus alvarezii extract and its optimal concentration in accelerating of Kappaphycus alvarezii micropropagule and embryo growth. Micropropagule and embryo produced through callus induction were planted into PES 1/20 liquid medium supplemented with seaweed extract at the concentrations of 0 (control), 25, 50, 75, and 100 μL in 10 mL of medium. The results showed that medium enrichment with 50 μL of seaweed extract had the highest survival rate and growth of thallus. In addition, this concentration was also resulted in a good performance of K. alvarezii thallus with the lighter color. The advantage of this study for seaweed cultivation in Indonesia, among others, seaweed can be used as fertilizer, especially in the maintenance of seaweed seed, so that cultivation can be better develop.
MARINE SPONGE Aaptos suberitoides, IT’S POTENTIAL SOURCE OF NATURAL ANTIBACTERIAL FOR CONTROLLING Vibrio harveyi ON TIGER SHRIMP (Penaeus monodon) CULTURE Rosmiati Rosmiati; Andi Parenrengi; Emma Suryati
Indonesian Aquaculture Journal Vol 10, No 1 (2015): (June 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (708.515 KB) | DOI: 10.15578/iaj.10.1.2015.33-40

Abstract

The study aims to isolate and identify the natural antibacterial compounds potential from Aaptos suberitoides for Vibrio harveyi control on tiger shrimp (Penaeus monodon) culture. The agar diffusion method using paper discs was used to determine the antibacterial activity of extracts (diethyl ether (DEE), butanol (BUE) and aqueous (HOE) and compounds successfully isolated against Vibrio harveyi. Findings showed that the antibacterial activity was concentrated in BUE with the inhibition zone of 17.2±0.1 mm. Meanwhile, two other extracts (DEE and HOE) did not exhibit any antibacterial activity against V. harveyi. From the active BUE, it was successfully isolated two compounds giving a strong anti-vibrio activity with the inhibition zone of 22±0.1 mm. The IR, 1H, 13C, COSY, HMQC, HMBC, and MS spectrum analysis indicated that both active compounds identified as aaptamine (1) and 9-demethyloxyaaptamine (2). The study suggested that marine sponge A. suberitoides may have potential compounds source for controlling of V. harveyi on tiger shrimp culture.
THE EFFECT OF MARINE SPONGE Aaptos aaptos EXTRACT IN VIBRIOSIS TREATMENT OF BLACK TIGER SHRIMP Penaeus monodon LARVAE Rosmiati Rosmiati; Najiah Musa; Habsah Mohammad; Tengku Sifzizul Tengku Muhammad; Andi Parenrengi; Wahyuni Wahyuni
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1778.016 KB) | DOI: 10.15578/iaj.9.2.2014.103-111

Abstract

Black tiger shrimp Penaeus monodon post larvae were challenged with Vibrio harveyi and butanol extract of selected marine sponge Aaptos aaptos to determine its antibacterial bioactive potential in vibriosis treatment. Based on the preliminary toxicity study, the A. aaptos butanol extract with concentrations of 31.25, 62.5, and 125 mg/L were selected in the study. Black tiger shrimp post larvae were challenged with V. harveyi at 107 cfu/mL and immersed A. aaptos butanol extract with the concentration of 125 mg/L showed significantly in (P<0.05) decrease mortality of the post larvae treated. Besides at this concentration, V. harveyi population in the rearing water and the post larvae treated decreased compared to control (untreated post larvae). Histological observation indicated that there was no changing on hepatopancreas of the black tiger shrimp post larvae. Based on this result, it is suggested that the butanol extract of A. aaptos is a potential bioactive compounds source in the treatment of vibriosis which may replaced the current antibiotics application.
Co-Authors Andi Parenrengi Andi Tenriulo Emma Suryati Habsah Mohammad Najiah Musa Tengku Sifzizul Tengku Muhammad Wahyuni Wahyuni