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selny Febrida
Balai Penelitian Pemuliaan Ikan, Jl. Raya 2 Sukamandi-Pantura Patok Beusi, Sukamandi-Subang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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THE ABILITY OF FAST-GROWING TRANSGENIC AFRICAN CATFISH (Clarias gariepinus) ON PREDATOR AVOIDANCE Huria Marnis; Bambang Iswanto; imron imron; selny Febrida; Raden Roro Sri Pudji Sinarni Dewi
Indonesian Aquaculture Journal Vol 11, No 1 (2016): (June 2016)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.204 KB) | DOI: 10.15578/iaj.11.1.2016.9-13

Abstract

Research Institute for Fish Breeding has produced transgenic African catfish (Clarias gariepinus) containing stripped catfish growth hormone gene (PccBA-PhGH) with growth 19.86% faster than that of non-transgenic fish. This fish has high potential to be released and utilized for fish farming sector to increase national production. However, there is not yet information about environmental risk of this fish. One of the major fitness traits determining potential environmental risk is predator avoidance. This study aimed to determine the predator avoidance ability of transgenic African catfish in an experimental laboratory condition. In this study, thirty five individuals each of transgenic and non-transgenic with body weight of about 0.1 ± 0.019 g were communally stocked in 60 cm x 40 cm x 40 cm aquarium with limited feeding frequency (ad libitum twice a day). One day after the fish were stocked, the predators were added to each aquarium. The non-transgenic and transgenic with body weight of 1.0 ± 0.024 g were stocked as predators as many as five individual in each aquarium. After approximately two weeks of predation, all remaining fish were collected for transgenic verification by PCR method. Genomic DNA was isolated from fin tissue of individually survivors. The results of this study showed that the transgenic fish had worse predator avoidance and lower cannibal than non-transgenic (P<0.05). There was no significant difference in growth performance between transgenic and non-transgenic African catfish (P>0.05) in limited food. The transgenic fish may have lower fitness than non-transgenic.
TRANSMISI GEN PhGH DAN PERFORMA PERTUMBUHAN IKAN LELE AFRIKA (Clarias gariepinus) TRANSGENIK GENERASI KETIGA Huria Marnis; Bambang Iswanto; Selny Febrida; Imron Imron; Raden Roro Sri Pudji Sinarni Dewi
Jurnal Riset Akuakultur Vol 11, No 3 (2016): (September 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (566.376 KB) | DOI: 10.15578/jra.11.3.2016.225-234

Abstract

Penelitian ini bertujuan untuk mengevaluasi transmisi transgen PhGH dan performa ikan lele Afrika transgenik generasi ketiga (F3) yang meliputi pertumbuhan, rasio efisiensi konversi pakan, konsentrasi hormon pertumbuhan dan hormon IGF-I. Ikan lele transgenik F3 telah diproduksi dengan meyilangkan ikan lele transgenik F2 dengan non-transgenik. Deteksi transgen (PhGH) dilakukan menggunakan metode PCR. Analisis hormon pertumbuhan dan hormon insuline-like growth factor (IGF-I) menggunakan sampel serum darah dan metode enzyme linked immunosorbent assay (ELISA). Hasil penelitian menunjukkan bahwa ikan lele transgenik F3 yang digunakan pada pengujian ini terdeteksi positif membawa transgen dengan ukuran fragmen gen sebesar 1.500 bp. Transmisi transgen dari induk F2 ke F3 berkisar 0-75%. Pertumbuhan bobot populasi ikan lele transgenik F3 lebih tinggi 51,26% dibandingkan dengan ikan lele non-transgenik (P<0,05). Pertumbuhan bobot populasi ikan transgenik mencapai 484±60,3 g, sedangkan pertumbuhan bobot ikan non-transgenik 319,98±65,3 g. Nilai rasio konversi pakan ikan lele transgenik F3 sebesar 0,89 sedangkan non-transgenik 1,30. Hal ini menunjukkan bahwa efisiensi pakan ikan lele transgenik F3 lebih tinggi dibandingkan dengan ikan non-transgenik (P<0,05). Ikan lele transgenik mempunyai konsentrasi hormon pertumbuhan (5,67±2,65 ng/mL) yang lebih tinggi (P<0,05) jika dibandingkan dengan ikan lele non-transgenik (3,00±1,41 ng/mL). Ikan lele transgenik juga memiliki kandungan hormon IGF-I (6,63±0,11 ng/mL) lebih tinggi (P<0,05) dibandingkan dengan ikan lele non-transgenik (5,38±0,63 ng/mL). Tingginya konsentrasi hormon pertumbuhan dan hormon IGF-I dapat mewakili performa pertumbuhan dan efisiensi penggunaan pakan pada ikan lele transgenik.The aim of this study was to determine the transmission of a transgene (PhGH) and to evaluate the performance of F3 transgenic African catfish, such as body weight, feed conversion ratio (FCR), growth hormone and IGF-I hormone profile. F3 transgenic were produced by mating F2 transgenic with non-transgenic fish. Detection of transgene was performed using PCR method. Analysis of the growth hormone and the insulin-like growth factor-I (IGF-I) hormone were conducted by enzyme linked immunosorbent assay (ELISA) method using serum samples. The results showed that the transgenic catfish F3 was positive carrying the transgene 1.500 bp. Transgene transmission from F2 to F3 ranged zero to 75%. The performance of F3 transgenic African catfish was significantly better 51.26% than that the non-transgenic (P<0.05). The body weight of transgenic population (484±255.25 g) was higher than that non-transgenic (319.98±165.27 g). FCR of transgenic fish (0.89) was lower than that non-transgenic (1.30). The growth hormone level of transgenic (5.67±2.65 ng/mL) was higher than that non-transgenic (3.00±1.41 ng/mL), IGF-I hormone level of F3 transgenic (6.63±0.11 ng/mL) was also higher than that non-transgenic (5.38±0.63 ng/mL). High level of growth hormone and IGF-I hormone represented both growth performance and efficiency of feed utilization of transgenic African catfish.
Co-Authors Bambang Iswanto Huria Marnis imron imron Raden Roro Sri Pudji Sinarni Dewi Raden Roro Sri Pudji Sinarni Dewi