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Rachmansyah Rachmansyah
Research Institute for Coastal Aquaculture, Maros
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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FERMENTED BLOOD MEAL USE FOR TIGER GROUPER, Epinephelus fuscoguttatus GROW-OUT DIET Usman Usman; Kamaruddin Kamaruddin; Neltje Nobertine Palinggi; Rachmansyah Rachmansyah; Taufik Ahmad
Indonesian Aquaculture Journal Vol 2, No 1 (2007): (June 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.698 KB) | DOI: 10.15578/iaj.2.1.2007.7-13

Abstract

The experiment aimed to evaluate the optimal level of fermented blood meal used in grow-out diets for tiger grouper, as an alternative protein source to fish meal. Juvenile tiger grouper, initial weight 31.1 ± 2.1 g, were stocked into 1 m x 1 m x 2 m floating net cages at 20 fish cage-1. The treatment applied was isoprotein and isocaloric diets formulated to contain fermented blood meal (FBM) of 0%, 7.5%, 15.0%, 22.5%, and 30.0% replacement of fish meal protein. The diets were fed to the fish twice a day to satiation for 20 weeks. Based on the Tukey test, the fish fed 0%–15.0% FBM demonstrated similar performance (P>0.05) to those fed the control diet (FBM0) in terms of specific growth rate, weight gain, and feed and protein efficiency. Specific growth rate, weight gain, feed efficiency and protein efficiency of the fish fed 22.5%–30.5% FBM were significantly lower (P<0.05) than those of the fish fed control diet (FBM0). However, there was a significant culvilinier decliner in overall fish performance with increasing inclusion of FPM and most notable for weight gain, feed efficiency and feed consumption. Based on regression analysis, the asymptote where fish growth deteriorates as a function of FBM inclusion was determined to be 8.9%. Tiger grouper diets incorporating up to 9% FBM as fish meal replacement had no adverse affects on fish growth and survival.
FLUCTUATION OF POST-PRANDIAL PLASMA MINERAL LEVEL OF JUVENILE JAPANESE FLOUNDER, Paralichthys olivaceus FED DIETARY PHOSPHORUS AND PHYTASE SUPPLEMENTATION Asda Laining; Rachmansyah Rachmansyah; Lideman Lideman; Shunsuke Koshio
Indonesian Aquaculture Journal Vol 5, No 2 (2010): (December 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.747 KB) | DOI: 10.15578/iaj.5.2.2010.117-131

Abstract

In order to investigate the phytic acid degradation in the gut of post juvenile Japanese flounder, indirect method was carried out by measuring the pre-prandial and postprandial plasma mineral and alkaline phosphatase (ALP) level as well as liver phosphorus content. The experiment was designed into a Randomized Block in which experiment units were grouped according to sampling days at 10, 20 and 30 days of feeding time. Experimental diets contained three levels of dietary inorganic phosphorus at 0.0; 0.25 and 0.5% combined with two levels of dietary phytase at 0 and 2,000 FTU/kg diet. Juvenile Japanese flounder (IBW = 36.2 g) were randomly distributed into 6 tanks of a 200 L capacity with density of 15 fish/tank. Blood sampling was carried out at 0 hour (before feeding or pre-prandial) and at 1, 3, 6 and 12 hour post feeding (post-prandial) time in three sampling days, respectively. Plasma was measured for mineral and ALP levels, while liver was analyzed for P content. The observation showed that fish fed without both dietary IP and phytase supplements had the lowest postprandial plasma IP, Mg and ALP levels during 12-h postprandial period. Plasma IP level at 6-h post-feeding in groups fed dietary 0.25 and 0.5% IP were significant higher when diet supplemented with phytase than those without phytase supplement. Peak level of plasma IP in fish fed 0.25% IP was similar to fish fed 0.5% with the presence of dietary phytase. At 1 and 3-h post-feeding, plasma Ca level increased in all groups, but significant difference was only observed between group fed diet without both dietary IP and phytase and other groups. Similar to plasma IP level, peak of plasma Mg and ALP concentration occurred in fish fed 0.25% IP together with phytase, and did not significantly differ from fish fed with 0.5% IP even when phytase was included in diet.
Co-Authors Asda Laining Kamaruddin Kamaruddin Lideman Lideman Neltje Nobertine Palinggi Shunsuke Koshio Taufik Ahmad Usman Usman