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All Journal JURNAL ILMU KEFARMASIAN INDONESIA
AGUSTINUS YUSWANTO
UNIVERSITAS GADJAH MADA
Medicine & Pharmacology

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Uji Aktivitas Fagositosis Makrofag Fraksi-fraksi dari Ekstrak Metanol Daun Sirih Merah (Piper crocatum Ruiz & Pav.) Secara In Vitro YUSTINA SRI HARTINI; SUBAGUS WAHYUONO; SITARINA WIDYARINI; AGUSTINUS YUSWANTO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 2 (2013): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Plants can be sources of immunomodulatory agents. Piper crocatum Ruiz & Pav. (red betel) leaves have been used to heal many diseases, especially to increase endurance. The aim of this research was to investigate the imunomodulatory effect of methanolic leaf extracts of Piper crocatum Ruiz & Pav. and its fractions by in vitro phagocytic macrophage activity test. The result showed that methanolic extract of Piper crocatum Ruiz & Pav. 75μg/mL could stimulate phagocytic macrophage activity equal to 100μg/mL product-X® that contained Echinacea extract. Separation of the extract by vacuum liquid chromatography using gradient solvent n-hexane - ethyl acetate yielded 5 fractions. The second fraction showed the highest activity. It contained alkaloid and terpenoid, while the extract also contained flavonoid and essential oil. Alkaloid and/or terpenoid could be the compound in the leaves of Piper crocatum Ruiz & Pav. that responsible increasing phagocytic macrophage activity.
Pemodelan Molekular Enzim 3β-Hydroxysteroid Dehydrogenase Tipe 2: Pemodelan Kombinasi Homologi, Docking dan Pendekatan QSAR BAMBANG SULISTYO ARI SUDARMANTO; AGUSTINUS YUSWANTO; RATNA ASMAH SUSIDARTI; SRI NOEGROHATI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 15 No 1 (2017): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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A homology model of human 3β-HSD type 2 has been developed from homology modeling techniques using Phyre2 server and refi ned by ModRefi ner. The PROCHECK, QMEAN and ProSA-web online tools were carried out to evaluate the stereochemical quality of the model. The Ramachandran plot resulted from PROCHECK showed that 84.5% residues are in the most favored region, 13.7% are in the additional allowed region, 1.5% are in the generously allowed region and 0.3% are in the disallowed region. The QMEAN (Z-score) are 0.509 (-3.006) and Z-score of ProSA-web is -7.10. The negative values of protein fold energies also found in almost all sequences. Furthermore, molecular docking was also applied to validate the model using MOE. The hydrogen bonding interactions with Tyr154, Ser124, and Ser218 are found in all docked substrates as well as known inhibitors (trilostane and epostane). A dataset of azasteroid inhibitors were also docked into the substrate active site of human 3β-HSD2. These docked structures were utilized to construct corresponding docking-based QSAR equation by employing genetic algorithm (GA) statistical analysis. The contructed best QSAR equation has a robust predictive power according to its statistical parameters, hence may be applied to supersede the default scoring function provided by docking software. These results indicate that the human 3β-HSD2 model was successfully evaluated as a good model.
Co-Authors BAMBANG SULISTYO ARI SUDARMANTO Ratna Asmah Susidarti Sitarina Widyarini Sri Noegrohati Subagus Wahyuono Yustina Sri Hartini