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Titi Parwati
Pusat Penelitian Bioteknologi-LIPI
Medicine & Pharmacology

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Studi Pendahuluan Mikroba Endofitik Penghasil Senyawa Terpen dari Tanaman Artenisia annua Titi Parwati; Bustanussalam Bustanussalam; Partomuan Simanjuntak
JURNAL ILMU KEFARMASIAN INDONESIA Vol 1 No 1 (2003): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Some endophytic microbes were isolated from medicinal plant Artemisia annua collected of Tawangmangu and Cibinong. Microbe isolate were made on corn meal malt agur supplemented with chloramphenicol and nutrient agar supplemented with nystatin from tissue subjected after the triple surface sterilization procedure. The potencies of these isolates to produce-terpene compounds were screenned by phytochemistry analysis, thin layer chromatography (tlc) and high performance liquid chromatography (hplc) after fermentation in four different medium. The results showed that 6 isolates from 17 microbe isolates can produce terpene compounds.
Isolasi dan Identifikasi Senyawa Hasil Fermentasi Kapang Endofit Pasak Bumi (Eurycoma longifolia Jack) Ratna Djamil; Titi Parwati; Nani Khomeni
JURNAL ILMU KEFARMASIAN INDONESIA Vol 2 No 2 (2004): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Endophytic fungi of Pasak bumi (Eurycoma longifolia Jack). were inoculated to synthetic medium and incubated for 28 days. The fumated medium then were extracted with chloroform. The antioxidant activities by 1,1-diphenyl 2-picrylhidrazyl (DPPH) methods showed that isolated compound has scavenging effect at IC285.7 ppm. Identification by interpretation of infra red spectra showed that the isolated compound was a terpenoid.
Isolasi, Elusidasi Struktur, dan Uji Bioaktivitas Alkaloid dari Akar Kemuning (Murraya exotica L.) Bustanussalam Bustanussalam; Titi Parwati; Partomuan Simanjuntak
JURNAL ILMU KEFARMASIAN INDONESIA Vol 2 No 2 (2004): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Isolation and structure elucidation of an alkaloid, a bioactive compound from the roots of the Indonesian medicinal plant Kemuning, Murraya exotica (Rutaceae), including the brine shrimp lethality test (BSLT) have been carried out. The bioactivity test using Artemia salina, isolation and purification of the ethyl acetate extract performed by column chromatography gave yellow crystals. Based on ultraviolet, infrared, nuclear magnetic resonance (1H-&13C-NMR) and gas chromatographymass spectra the isolated bioactive compound is an indole alkaloid typical to yuehchukene.
Isolasi dan Identifikasi Senyawa Antioksidan dari Ekstrak Benalu Teh (Scurrula oortiana (Korth) Danser) Partomuan Simanjuntak; Titi Parwati; Lisya Evy Lenny; Swasono R. Tamat; Retno Murwani
JURNAL ILMU KEFARMASIAN INDONESIA Vol 2 No 1 (2004): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

The leaves of tea benalu (Scurrula oortiana (Korth) Danser) were extracted with nhexane, ethyl acetate, methanol, and water respectively. The antioxidant activities were measured using 1,1-diphenyl-2-picrylhidrazyl (DPPH) methods. The result showed that the isolated compound has scavenging effect with IC50 92.22 ppm. Identification by interpretation of ultraviolet, infrared, and nuclear magnetic resonance spectra showed that the isolated compound was cathecin.
Co-Authors Bustanussalam Bustanussalam Lisya Evy Lenny Nani Khomeni Partomuan Simanjuntak Ratna Djamil Retno Murwani Swasono R. Tamat