Article Per Year (5 Year)
p-Index From 2019 - 2024
0.562
P-Index
This Author published in this journals
All Journal Journal of the Indonesian Tropical Animal Agriculture
M. A. Setiadi, M. A.
Faculty of Veterinary Medicine, Bogor Agricultural University, Jln. Agatis, Darmaga Campus, Bogor 16680
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
Transformation of ram sperm nuclei in oocytes cytoplasm during in vitro fertilization Dzulfiqor, Y.; Setiadi, M. A.; Karja, N. W. K.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 2 (2019): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.2.146-154

Abstract

The aim of present study was to understand the transformation of ram sperm nuclei within oocyte cytoplasm during in vitro fertilization. The oocytes were collected from slaughterhouse ovaries. Before fertilization, the oocytes were maturated in vitro for 24 hours in the incubator with 5% CO2 at 38.5°C. Then the oocytes (n= 635) was fertilized by incubating the oocytes with sperm (5x106 spermatozoa/ ml) for 3, 6, 9, 12, and 15 hours. At the end of incubating period, the oocytes were fixed and stained with aceto-orcein 2% before evaluated under phase contrast microscope. Sperm nuclear transformation was evaluated according to sperm nuclear status of sperm, such as condensation, decondensation, and formation of prepronuclei and pronuclei. Sperm condensation and decondensation were seen at 3 hours after incubation. Prepronuclei and pronuclei were found at 6 hours of incubation. Pronuclei formation was significantly increased in the 9 hours after incubation (P<0.05). The incidence of polyspermia was significantly increased at 12-15 hours after incubation (P<0.05). In conclusion penetration of sperm into oocytes has been occurred at 3 hour of fertilization period. The formation of pronuclei was found at 6 hours after incubation and the incidence of polyspermia was increased when the fertilization period prolonged.
NUCLEAR MATURATION RATE OF SHEEP OOCYTES IN VITRO: EFFECT OF STORAGE DURATION AND OVARY TEMPERATURE Febretrisiana, A.; Setiadi, M. A.; Karja, N. W. K.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 2 (2015): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.2.93-99

Abstract

The present study was conducted to investigate the effects of duration and temperature storageof sheep ovaries on nuclear maturation in vitro of oocytes obtained from stored ovaries. Ovaries werecollected from slaughterhouse and stored in physiological saline for 2-4 h, 5-7 h and 8-10 h at varioustemperatures (27-28°, 36-37° and 4°C). Cumulus oocyte complexes (COCs) were collected with slicingmethod from each group and matured in vitro for 26 h. There was no difference between the proportionsof oocytes maturation to metaphase II when collected from ovaries stored at 27-28°C and 36-37°C for 2-7 h of slaughter. However, the percentages of oocytes from ovaries stored at 4°C were significantlylower (P<0.05) than those stored at higher temperature (69.23%, 70.83% and 45.65%, for 27-28, 36-37and 4°C at 2-4 h, respectively; 59.61%, 64,58% and 36.36% for 27-28, 36-37 and 4°C at 5-7 h,respectively). The proportion of oocytes meiosis to metaphase II (MII) were significantly (P<0.05)decreased when the ovaries stored at 27-28 and 36-37°C for 8-10 h, but not in oocytes stored at 4°C(24.37%, 7.84% and 45.23%, respectively). The maturation rate of oocytes from ovary stored at 4°C washigher than those collected from ovaries stored at higher temperature (P<0.05). This finding indicated that the storage of ovaries at 4°C for 8-10 h is effective for maintaining the development competence ofsheep oocyte better than storage at higher temperature.
Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times Amal, A. S.; Arifiantini, R. I.; Setiadi, M. A.; Said, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 2 (2019): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.2.135-145

Abstract

The objectives of the present study were to compare and determine the best post-thawed characteristics of balinese bull sperm cryopreserved in three different extenders; animal based (Tris-clarified egg yolk (Tris-cEY)), and non-animal based extenders (Bioxcell® (lecithin based) and Optixcell® (liposome based)) in combination with three different equilibration times (30 minutes, 2 hours, 4hours). Thirty six ejaculates were collected from six Balinese bulls and frozen in three extenders (Tris-cEY, Bioxcell® and Optixcell®) after equilibration in three different times (30 minutes, 2hours and 4hours). Computer-assisted sperm analysis (CASA), hypo-osmotic swelling test (HOST) and eosin nigrosin staining were used in the post-thawed semen analysis. There was a significant interaction between equilibration time and extender type for sperm motility, viability and membrane integrity. Thirty minutes equilibration time had the lowest values (P<0.05) for all the evaluated parameters independent of extender type. Overall, semen extended in Tris-cEY, Bioxcell® and Optixcell® were similarly better when equilibrated at 4 hours (P>0.05). Moreover, post-thawed semen which were extended in Optixcell® for 2 hours equilibration showed a better motility compared with the other extenders (P<0.05). In conclusion, two hours equilibration of semen with Optixcell® is sufficient for semen freezing. Four hours equilibration has the best sperm survival, independent of the extender type.
Sex sorting sperm of sumba ongole bulls by using snakehead fish (Channa striata) albumin extract Maulana, T.; Said, S.; Arifiantini, R. I.; Setiadi, M. A.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 1 (2019): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.1.106-113

Abstract

The objective of this research was to investigate the potential of snakehead albumin extract (channalbumin) for sorting X and Y sperm of Sumba Ongole (SO) and its characteristic. Semen was collected from three SO bulls using artificial vagina and the freeze dried channalbumin was extracted from snakehead fish. Channalbumin column was made with different concentration ratio of top and bottom fraction: 2%:4%; 3%:5%; 4%:6% respectively and BSA 5%:10% as control. Semen was put in top fraction then incubated for 30 min at room temperature then each fraction was centrifuged at 1800 rpm for 10 minutes. The pellet was evaluated for motility, abnormality, viability, membrane integrity and head sperm morphometric. The results showed that the channalbumin capable to maintain sperm motility in the top fraction better than the bottom fraction. Sperm viability and membrane integrity in control group were significantly higher (P<0.05) than all channalbumin treatment. BSA 5%:10% has highest proportion of X and Y sperm (69%:76.77%) compared with 2%:4% (42.33%:79.13%), 3%:5% (55.97%:75.73%) and 4%:6% of channalbumin (62.77%:68%). It’s concluded that channalbumin 4%: 6% was effective for separation of XY sperm with higher proportion.
Co-Authors A. Febretrisiana, A. Amal, A. S. Dzulfiqor, Y. N. W. K. Karja, N. W. K. R. I. Arifiantini S. Said T. Maulana, T.