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S. Said
Research Center for Biotechnology - Indonesian Institute of Sciences, Jl. Raya Bogor KM. 46, Cibinong 16911, West Java
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Journal : Journal of the Indonesian Tropical Animal Agriculture

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Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times Amal, A. S.; Arifiantini, R. I.; Setiadi, M. A.; Said, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 2 (2019): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.2.135-145

Abstract

The objectives of the present study were to compare and determine the best post-thawed characteristics of balinese bull sperm cryopreserved in three different extenders; animal based (Tris-clarified egg yolk (Tris-cEY)), and non-animal based extenders (Bioxcell® (lecithin based) and Optixcell® (liposome based)) in combination with three different equilibration times (30 minutes, 2 hours, 4hours). Thirty six ejaculates were collected from six Balinese bulls and frozen in three extenders (Tris-cEY, Bioxcell® and Optixcell®) after equilibration in three different times (30 minutes, 2hours and 4hours). Computer-assisted sperm analysis (CASA), hypo-osmotic swelling test (HOST) and eosin nigrosin staining were used in the post-thawed semen analysis. There was a significant interaction between equilibration time and extender type for sperm motility, viability and membrane integrity. Thirty minutes equilibration time had the lowest values (P<0.05) for all the evaluated parameters independent of extender type. Overall, semen extended in Tris-cEY, Bioxcell® and Optixcell® were similarly better when equilibrated at 4 hours (P>0.05). Moreover, post-thawed semen which were extended in Optixcell® for 2 hours equilibration showed a better motility compared with the other extenders (P<0.05). In conclusion, two hours equilibration of semen with Optixcell® is sufficient for semen freezing. Four hours equilibration has the best sperm survival, independent of the extender type.
THE POTENCY OF SUMBA ONGOLE (SO) CATTLE: A STUDY OF GENETIC CHARACTERIZATION AND CARCASS PRODUCTIVITY Agung, P. P.; Anwar, S.; Wulandari, A. S.; Sudiro, A.; Said, S.; Tappa, B.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 2 (2015): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.2.71-78

Abstract

A study was conducted to assess the genetic characterization of the Sumba Ongole (SO) cattlebased on DNA microsatellites and also to study the potency of SO cattle based on carcass productivity.Blood samples were collected from 28 individual cattle and 12 microsatellite primers as recommendedby FAO were used to identify the genetic characterization of the SO cattle population. Data of carcassproductivity were collected from 506 individual cattle that slaughtered in Karawaci abattoir, Banten,Indonesia. The heterozygosity values of microsatellite loci ranged from 0.143 to 1.000 (mean 0.536).The highest PIC values was 0.814 (locus TGLA122), while the lowest was 0.280 (locus BM1818).Cattle in range of 351-475 kg slaughter weight was most slaughtered in year 2013 and 2014 with carcasspercentage ranged from 52.89% to 53.43%. The highest carcass percentage (56.34%) was obtained fromcattle in range of 626-650 kg slaughter weight while the lowest (51.42%) was obtained from cattle inrange of 250-275 kg slaughter weight. The results of genetic characterization showed that allmicrosatellite locus were highly polymorphic and highly informative for detecting the level of geneticdiversity in the SO cattle population. The results of carcass productivity showed that the SO cattle hasexcellent potential as beef cattle compare with other local breeds cattle in Indonesia.
Sex sorting sperm of sumba ongole bulls by using snakehead fish (Channa striata) albumin extract Maulana, T.; Said, S.; Arifiantini, R. I.; Setiadi, M. A.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 1 (2019): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.1.106-113

Abstract

The objective of this research was to investigate the potential of snakehead albumin extract (channalbumin) for sorting X and Y sperm of Sumba Ongole (SO) and its characteristic. Semen was collected from three SO bulls using artificial vagina and the freeze dried channalbumin was extracted from snakehead fish. Channalbumin column was made with different concentration ratio of top and bottom fraction: 2%:4%; 3%:5%; 4%:6% respectively and BSA 5%:10% as control. Semen was put in top fraction then incubated for 30 min at room temperature then each fraction was centrifuged at 1800 rpm for 10 minutes. The pellet was evaluated for motility, abnormality, viability, membrane integrity and head sperm morphometric. The results showed that the channalbumin capable to maintain sperm motility in the top fraction better than the bottom fraction. Sperm viability and membrane integrity in control group were significantly higher (P<0.05) than all channalbumin treatment. BSA 5%:10% has highest proportion of X and Y sperm (69%:76.77%) compared with 2%:4% (42.33%:79.13%), 3%:5% (55.97%:75.73%) and 4%:6% of channalbumin (62.77%:68%). It’s concluded that channalbumin 4%: 6% was effective for separation of XY sperm with higher proportion.
CONCEPTION RATES AND SEX CONCOMITANT OF BALI CALVES FOLLOWING OESTRUS SYNCHRONIZATION AND ARTIFICIAL INSEMINATION OF FROZEN- SEXED SEMEN UNDER FARM CONDITIONS Said, S.; Arman, C.; Tappa, B.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 1 (2014): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.1.10-16

Abstract

The objective of this study was to determine the conception rate of Bali cattle synchronized andartificially inseminated with frozen thawed sexed-semen concomitant with its calves sex. Thisexperiment was conducted in two cattle farms in West Lombok Regency. Oestrus was synchronized witha single injection i.m. of 5 cc Lutalyse ® containing 25 mg of PGF 2α (Lutalyse, Sterile Solution,Pharmacia Animal Health). The observation was conducted in the responded cattle that artificiallyinseminated at once oestrus. Pregnancy diagnosis was conducted by rectal palpation at about day-90. InFarm 1, the conception rate (CR) of PGF 2α -treated and non-treated animals were 29/63 (46.03%) and71/75 (94.66%), respectively. However, in Farm 2 the corresponding values were 38/89 (42.70%) and10/23 (43.48%), respectively. CR in both farms were found being 100%. Sex concomitant rate forfemale calves born in Farm 1 was found being 94/100 (94.00%), whilst in Farm 2, sex concomitant ratefor male calves born was recorded to be 45/48 (93.75%). It was concluded that under farm conditionsoestrus synchronization using PGF 2α in conjunction with AI of frozen-thawed Bali sexed spermatozoamight be used with acceptable conception and calving rates that above 90 % concomitant with itsrespective calve-sex rates.
Genetic polymorphism analysis of 5' untranslated region of thyroglobulin gene in Bali cattle (Bos javanicus) from three different regions of Indonesia Anwar, S.; Putra, A.C.; Wulandari, A.S.; Agung, P. P.; Putra, W.P.B.; Said, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 3 (2017): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.3.175-184

Abstract

The g.422C>T nucleotide variations in the 5’ untranslated region (5’UTR) of TG gene (called as TG5) has been reported to be associated with level in intramuscular fat (IMF) content or marbling in beef cattle. The objective of this study was to confirm genetic polymorphism of TG5 gene in Bali cattle populations from three different regions as the main resources of Bali cattle in Indonesia. A total of 200 head of Bali cattle have been performed genotyping on TG5 gene using polymerase chain reaction-restriction fragment lenght polymorphism (PCR-RFLP) method and sequence analysis. Results of the study confirmed that TG5 was monomorphic in Bali cattle wherever their origin regions. Moreover, nine candidate SNPs were detected within 5’UTR of TG gene in Bali cattle compared to Genbank reference sequences, although no SNP variations among Bali cattle sample studied. The new other genetic markers within an entire TG gene suggested to be explored and verified for their polymorphisms in Bali cattle. The nine candidate SNPs were also required further verification and validation in a larger sample to be regarded as new SNPs between Bali cattle and Genbank reference sequences.
Seminal plasma protein profile based on molecular weight and their correlation with semen quality of Simmental bull Baharun, A.; Arifiantini, I.; Karja, N. W. K.; Said, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 46, No 1 (2021): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.46.1.20-28

Abstract

Artificial Insemination Center (AIC) produces frozen semen that was taken from superior bull. The selection of superior bull was based on breeding soundness evaluation. This study is aimed to evaluating the correlation between the molecular weight (MW) of seminal plasma protein and semen quality of Simmental bulls. Semen ejaculates from nine Simmental Bulls, that belong to AIC of Central Java, at Ungaran, were collected by using an artificial vagina. The sub population of sperm in ejaculates was evaluated through macroscopically and microscopically, in which the semen then centrifugated 6500 rpm for 30 minutes. The supernatant was collected and inserted into mini straw and stored in liquid nitrogen container. The concentration of seminal plasma protein was determined by the Bradford method. The Bradford protocol of analysis was suitable with User Guide Coomasize (Bradford) Protein Assay Kit. Thermo Skanlt RE for Multiskan Go Software, 3.2 version was applied for analyzing of the data, and protein characterization used was 1D-SDS-PAGE. The Coomassie Brilliant Blue was used for coloring the gels, and the proteins MW was determined by MW markers. The result demonstrated that there was no significant correlation between protein concentration and semen quality (P>0.05). Protein expressions based on MW were 62-48 kD, 100-71 kD found in this study. The analysis of correlation showed that the proteins with 100-71 kD MW correlated with sperm motility, normal sperm morphology, and sperm concentration. Semen quality in all parameters was significant (P˂0.05 and P>0.01) with 62-48 kD protein MW. The result concluded that seminal plasma protein had a correlation with semen quality and can be used as additional indicator for bull’s candidate selection. 
Co-Authors A. S. Wulandari, A. S. A. Sudiro, A. Agung, P.P. Amal, A. S. B. Tappa Baharun, A. C. Arman Christopher, E. F. Afiati, F. I. Arifiantini Ibrahim, G.E. Kantong, S. A. M. A. Setiadi, M. A. M. S. A. Zein, M. S. A. Muzawar, M. N. W. K. Karja, N. W. K. P. P. Agung, P. P. Putra, A.C. R. I. Arifiantini Ramadhan, M.N. S. Anwar Setiorini, S. Sophian, E. T. Maulana, T. Volkandari, S. D. W. P. B. Putra W.P.B. Putra Wulandari, A.S.