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All Journal INTEK: Jurnal Penelitian
Ratih Indrawati
Department of Health Analyst, Poltekkes Kemenkes Pontianak
Computer Science & IT Engineering

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Senggani Fruit Anthocyanins (Melastoma Malabathricum Auct, Non Linn) As Bacterial Dyes Differential Painting Techniques Ratih Indrawati; Gervacia Jenny Ratnawati; Sri Tumpuk
INTEK: Jurnal Penelitian Vol 9, No 1 (2022): April 2022
Publisher : Politeknik Negeri Ujung Pandang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31963/intek.v9i1.2987

Abstract

Bacteria are difficult to see with a light microscope, because they do not absorb or refract light. Dyes absorb and refract light so that the contrast of bacteria with their surroundings is enhanced. Anthocyanins are water-soluble pigments that are naturally found in various types of plants. As the name implies, this pigment gives color to flowers, fruits, and leaves of green plants.This research is a type of descriptive research. The treatment consisted of painting bacterial preparations with anthocyanin pigment extract of senggani fruit with 70% ethanol solvent and control with gram staining. The treatments were: T1: staining of bacterial preparations with gram staining, T2: painting of bacterial preparations with anthocyanin extract of senggani fruit added with 14% citric acid as a substitute for safranin and NH4Cl as a substitute for crystal violet. The results of the study showed that anthocyanin pigment extract was proven to be used as a dye for Staphylococcus aureus, Escereria coli as a substitute for synthetic dyes Safranin and Crystal violet in Gram staining.
Co-Authors Gervacia Jenny Ratnawati Sri Tumpuk