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Paternity Analysis of Tea (Camellia sinensis L. Kuntz) Hybrids Using Isozyme Marker Titin Setyorini; T. Taryono; S. Suyadi; Sapto Indrioko
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.598 KB) | DOI: 10.22146/ijbiotech.7857

Abstract

Tea plant has been categorized as self-incompatible crop. This is the reason behind the high genetic diversity. Natural pollination is possible to occur and the male parent is usually unknown, therefore, there is a need of method to identify male parent of hybrids through paternity analysis. Isozyme markers have been successfully used for paternity analysis due to their co-dominant polymorphism. This research aimed to predict male parents of hybrids by figuring out the mating system through isozyme banding patterns. In this experiment, seven enzyme systems were evaluated, of which only two of the enzyme systems i.e. esterase and shikimate dehydrogenase showing clear band pattern of Est-1, Est-2, and Shd-1 loci. The mating system of tea could be categorized as a mixed mating model, with high estimated out-crossing rate of 98.6 %. The pollen contributors were not always originated from the vicinity of the female parents.Key words: isozyme markers, paternity analysis, tea
Allozyme variation of the endemic and vulnerable Dyera lowii Hook.f. in Central Kalimantan: Implications for genetic resources conservation Tri Suwarni Wahyudiningsih; Mohammad Naiem; Sapto Indrioko; Issirep Sumardi
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.811 KB) | DOI: 10.22146/ijbiotech.8637

Abstract

Dyera lowii is an endemic and vulnerable tree species of commercial value as chewing gum found inpeat swamp forests, scatteredly distributed in Sumatra, Kalimantan, and Peninsular Malaysia. Their existenceis now under severe threat due to habitat conversion. This study is aimed to assess genetic diversity withinfour natural populations (Hampangen, Parahangan, Sebangau, Selat Nusa ) and one plantation in CentralKalimantan based on allozyme variation. Electrophoresis procedures were conducted with an isoelectricfocusing polyacrylamide slab gel system. The result showed high genetic diversity (HE=0.52) and gene fl ow(3.402) seemed to be effective. A total of 14 alleles were found among all the analysed population. Meannumber of alleles per locus (Aa) was 3.206, and the effective number of alleles per locus (Ae) was 2.21. Geneticdifferentiation between populations (FST) was signifi cant at the moderately level (0.0685). Most allozymevariation was found within population (93.2%). Special attention is essential to conserve a private allele ofGot-1-e (9%) at Selat Nusa population. Sebangau population missed the alleles of Est-2-b and Got-1-a, as foundin other populations. Selat Nusa population is expected to enhance the effective management for geneticresources conservation of this vulnerable species in the future.
ESTIMASI LAJU PERKAWINAN SILANG PADA Toona sinensis Roem DENGAN PENANDA RAPD (Random Amplified Piolymorphic DNA) [Out crossing value estimation in Toona sinensis Roem Based On RAPD Markers] Jayusman Jayusman; Muhammad Na'iem; Sapto Indrioko; Eko Bhakti Hardianto; ILG Nurcahyaningsih
Journal Penelitian Kehutanan FALOAK Vol 2, No 1 (2018): Jurnal Penelitian Kehutanan FALOAK
Publisher : Balai Penelitian Dan Pengembangan Teknologi Hasil Hutan Bukan Kayu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (675.966 KB) | DOI: 10.20886/jpkf.2018.2.1.13-28

Abstract

ABSTRACTToona sinensis Roem or surian, belongs to the family Meliaceae. It is very important Indonesia community forest tree species because of it excellent wood quality and medicinal properties.  The study investigated the mating system of surianbase on 88 polymorphic band Random Amplified PolymorphicDNA (RAPD)of 200 tree sample. Origin of geographical location and materials used in this research from Enrekang seed stand identified population, South Sulawesi Province.The mating parameter estimated using the mix mating model showed that this population practice a mixture of mating model with multilocus outcrossing  rate (tm)was 0.938, meaning that  the total of outcrossing that can be among relative individual or not. The singlelocus outcrossing rate (ts) was 0.765, meaning that most outcrossing occurred among non-relative tree than among relative ones (tm- tm)= 0,172. Highest value was obtained for correlation of selfing among families (0.999±0.045), but low value obtained forcorrelation paternity multilocus (0.076±0.016) and correlation paternity singlelocus (-0.002±0.062).Maternal inbreeding coefficient (Fm)was 0.032, meaning high category. From inbreed crossing  0,062 (s=1-tm) was because selfing. Therefore we can concluded that surian is low tolerance of selfing.These result have important implication to establish strategies of genetic breeding strategic of surian. ABSTRAK Toona sinensis Roem atau surian, termasuk keluarga Meliaceae. Spesies hutan rakyat yang sangat penting di Indonesia karena memiliki kualitas kayu dan memiliki banyak manfaat sebagai obat. Penelitian ini meneliti sistem perkawinan berdasarkan 88 pita polimorfik Random Amplified Polymorphic DNA (RAPD) dari 200 sampel pohon. Asal lokasi geografis dan bahan yang digunakan dalam penelitian ini adalah dari populasi tegakan benih teridentifikasi Enrekang, Provinsi Sulawesi Selatan. Estimasi parameter mating sistem menggunakan model kawin campuran menunjukkan bahwa populasi ini mempraktekkan model kawin campuran dengan nilai outcrossing(tm) 0,938, yang berarti bahwa total perkawina silang pada banyak lokus dapat terjadi di antara individu berkerabat atau  tidak berkerabat. Tingkat outcrossing lokus tunggal (ts) 0,765 yang berarti bahwa hubungan yang paling jauh terjadi di antara pohon tidak berkerabat  daripada yang relatif (tm - tm) = 0,172. Nilai tertinggi ditunjukkan pada  korelasi selfing antar keluarga (0,999 ± 0,045) dan koefisien kawin kerabat  (Fm) 0,332. Sedangkan nilai rendah ditunjukkan korelasi paternal multilokus (0,076 ± 0,016), korelasi lokus tunggal (-0.002 ± 0.062). Nilaiselfing (s =1-tm) 0,062. Oleh karena itu dapat disimpulkan bahwa surian (T. Sinensis Roem) adalah memiliki sifat toleransi rendah terhadap selfing. Hasil ini memiliki implikasi penting untuk menetapkan strategi pemuliaan genetik surian.