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Sapto Raharjo
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Haluoleo University, Kendari
Mathematics

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Development of Affinity Adsorbent System for Purification of Protease Abdul Haris Watoni; Sapto Raharjo; Husein H. Bahti; Soetijoso Soemitro
Jurnal Matematika & Sains Vol 7, No 1 (2002)
Publisher : Institut Teknologi Bandung

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Abstract

The development of affinity adsorbent system for purification of protease produced by Bacillus sp. BAC-4 has been investigated. The research is initiated from preparation of non porous silica, aminopropyl silica matrix, and affinity adsorbent. In this research, the approximately spherical form of non porous silica with diameter of 1 – 15 μm and aminopropyl silica matrix has been synthesized. This matrices contains 65,7 μmol/g of amino group and show the similar FTIR spectrum to that of standard aminopropyl silica. Preparation result of affinity adsorbent shows that 10% n-butanol is the most applicable affinity ligand with 99,96% binding capacity to the matrix. The optimum activity of the purified protease with affinity adsorbent system is in the 0.05 N Tris HCl buffer pH 8.0 at 550C for 15 minutes with the degree of purity of 1248–fold to that in the crude extract.
Co-Authors Abdul Haris Watoni Husein Hernadi Bahti Soetijoso Soemitro