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All Journal ANNALES BOGORIENSES JURNAL PHOTON Teknologi Indonesia Proceeding Biology Education Conference
Martha Sari
Pusat Penelitian Bioteknologi LIPI, Cibinong
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health

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Preparation of An scFv-Based Immunoliposome Specific towards Transferrin Receptor Kusharyoto, Wien; Handayani, Ira; Sari, Martha; Fuad, Asrul Muhamad
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (664.3 KB) | DOI: 10.1234/99

Abstract

An ideal therapeutic for cancer would be one that selectively targets to tumor cells, is nontoxic to normal cells, and that could be systemically delivered, thereby reaching metastases as well as primary tumor. Immunoliposomes directed by monoclonal antibody or its fragments are promising vehicles for tumor targeted drug delivery. Transferrin receptors (TfR) levels are elevated in various types of cancer cells and considered to correlate with the aggressive or proliferative ability of tumor cells. Therefore, TfR levels can be elaborated as a prognostic tumor marker, and TfR is a potential target for drug delivery in the therapy of malignant cells. Here, we report the preparation of an anti-TfR single-chain antibody variable (scFv) immunoliposome for tumortargeted delivery vehicle. The cDNA encoding the variable heavy and light chain domains of the anti-TfRscFv antibody fragment was derived from the murine monoclonal antibody Clone E6, which is specific towards transferrin receptor. The gene encoding the anti-TfR scFv fragment was codon optimized for expression inEscherichia coli, subsequently synthesized, and cloned into the expression vector pJexpress404. The His6- tagged anti-TfR scFv fragment was expressed in E. coli and purified by means of immobilized metal-ion  affinity chromatography on TALON™ matrix. SDS-PAGE revealed that the scFv fragment had the size of approximately 27 kDa, which corresponded with the predicted size of the protein based on its amino acid sequence. Liposome containing 5% MPB-DOPE were prepared by ethanol injection method. Afterwards, the anti-TfR scFv fragments were covalently conjugated to the liposome to produce the anti-TfR scFv immunoliposome with the size of around 200 to 300 nm.
Effect of culture conditions on phytase production by Aspergillus ficuum in solid-state fermention using rice bran as substrate Kusharyoto, Wien; Sari, Martha; Ridwanuloh, Asep Muhammad
ANNALES BOGORIENSES Vol 13, No 1 (2009): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.142 KB) | DOI: 10.1234/23

Abstract

Phytic acid is an antinutritional factor that forms 1–2% of most of the seeds and their co-products representing more than 60%  of their total phosphorus. Monogastric and agastric animals are unable to utilize phytate phosphorus either due to lack of or insufficient amount of phytate degrading enzymes. Phytases (myo-inositol hexakisphosphate-phosphohydrolase) are a special class of phosphatases that catalyze the hydrolysis of phytic acid in a stepwise manner to lower inositol phosphates, myo-inositol and inorganic phosphate. Phytases are found naturally in plants and microorganisms and a sizeable number of phytases have been purified and characterized from various fungi, yeasts and bacteria. The present investigation involves studies on the effect of moisture content, pH value and different media ingredients such as carbon, nitrogen, and surfactants on the production of phytase by the fungus  Aspergillus ficuum DSM 932 in solid-state fermentation (SSF) using rice bran as substrate. The production of phytase by SSF was favored, when the fungus was grown at a moisture content of 60% and pH 7.0, resulted in a phytase activity of 5.2 units/g dry substrate. There was a 20% increase in phytase yield in the presence of sucrose in SSF medium, while glucose and fructose were not effective in enhancing the phytase activity when used individually. Yeast extract was found to be a favorable nitrogen source for phytase production by SSF, which resulted in a 20% increase in phytase activity. There was no significant effect in increasing phytase production with the use of either soy peptone or tryptic  soy as nitrogen source. Approximately 30% inhibition in phytase activity was shown in the presence of the surfactant Tween-80 or Triton X-100 in the SSF. By supplementing rice bran with sucrose and yeast extract, and performing the SSF in tray bioreactors, a phytase activity of 6.76 units/g dry substrate could be obtained.Keywords:  phytase, solid-state fermentation, Aspergillus ficuum, nutritional factors, rice bran
PENINGKATKAN KUALITAS MINYAK GORENG CURAH MENGGUNAKAN ADSORBEN LEMPUNG DESA GEMA TERAKTIVASI Sophia, Halida; Muchtar, Akmal; Sari, Martha
Sistem Informasi Vol 5 No 1 (2014): Jurnal Photon
Publisher : Fakultas MIPA dan Kesehatan Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (495.302 KB)

Abstract

Telah dilakukan penelitian tentang peningkatan mutu minyak goreng curah menggunakan adsorben lempung asal Desa Gema, Kabupaten Kampar, Provinsi Riau. Penelitian ini meliputi: (1) Pembuatan adsorben dengan aktivasi menggunakan H2SO4 0,5 M, selama 3 jam dengan kecepatan pengadukan 500 rpm (2) Pemurnian minyak goreng secara adsorbsi dan mengetahui mutu minyak curah setelah adsorbsi berupa bilangan peroksida, bilangan asam, kandungan air, warna. Proses adsorpsi diamati dengan variasi massa (1, 3, 5, dan 7 gram) dengan waktu kontak 30 menit. Semua nilai yang diperoleh dibandingkan dengan SNI 3741:2013. Hasil yang didapat menunjukkan lempung teraktivasi H2SO4 mampu menurunkan bilangan peroksida, bilangan asam, dan kandungan air secara maksimal masing-masing sebesar 31,02%, 36,36%, dan 38,89% dengan 7 gram lempung teraktivasi.
STRUCTURAL CHARACTERIZATION OF FRACTIONATED GLYCOLIPID BIOSURFACTANTS SYNTHESIZED BY Pseudozyma aphidis YB205 Sari, Martha; Kartika, I Made; Kusharyoto, Wien
Teknologi Indonesia Vol 39, No 3 (2016)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v39i3.278

Abstract

Fractionation and structural characterization of glycolipid biosurfactant produced by yeast Pseudozyma aphidis strain YB205 was conducted. The yeast strain was grown in a nutrient broth with crude oil as the carbon sources and the glycolipid biosurfactant produced was isolated. The crude glycolipid was fractionated using column chromatography followed by complete separation and purification using extraction technique employing  different organic solvents. The fractions were subjected to activity test using oil displacement assay followed by chemical identity test using thin layer chromatography. In order to elucidate its chemical structure, the most active fraction was subjected NMR and FTIR analysis. Results showed that six major fractions were generated all of which showed biosurfactant activity. Four fractions is fractions 2, 4, 5, and 6 showed glycolipid characteristics and fraction 6 showed the highest biosurfactant activity. Combination of NMR and FTIR spectroscopy spectra indicated that chemical structures of fraction 6 belonged to glycolipid species.
ISOLASI DAN SELEKSI BAKTERI PENGHASIL BIOSURFAKTAN DARI SAMPEL LUMPUR MINYAK, KALIMANTAN TIMUR Sari, Martha; Afiati, Fifi; Kusharyoto, Wien
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Abstract- A total of six bacteria isolated from waste of oil sludge in the area of East Kalimantan were examined to confirm the ability in biosurfactant activity. The active compounds from each bacteria were subjected to screening test of biosurfactant by usingcanola oil as substratein fermentation system. The present study aims to evaluatedthe initial potency ofbiosurfactant producing bacteria from sludge oil in East Kalimantan. All of the isolates were confirmed as producers of biosurfactants with diverse of emulsification activity. Strain SMF4 showed the highest emulsification activity and oil displacement test within 9 days of cultivation.The screening results revealed that the six strains of sludge oil bacteria gave good values in emulsification and oil displacement activity. Keywords: oil sludge, six bacteria, emulsification, and biosurfactant
Co-Authors Akmal Muchtar Asrul Muhamad Fuad, Asrul Muhamad Fifi Afiati Halida Sophia Handayani, Ira I Made Kartika Kusharyoto, Wien Kusharyoto, Wien Kusharyoto, Wien Ridwanuloh, Asep Muhammad