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Yusianti Silviani
SEKOLAH TINGGI ILMU KESEHATAN NASIONAL
Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Jurnal Kesehatan Kusuma Husada

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PENGARUH PERBEDAAN SUHU DAN LAMA PENYIMPANAN SPUTUM TERHADAP JUMLAH BAKTERI TAHAN ASAM Dwi Handayani; Yusianti Silviani
Jurnal Kesehatan Kusuma Husada Vol. 13 No. 2, Juli 2022
Publisher : Universitas Kusuma Husada Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.601 KB) | DOI: 10.34035/jk.v13i2.859

Abstract

Tuberkulosis merupakan suatu penyakit menular langsung yang disebabkan oleh bakteri TB yaitu Myobacterium Tuberculosis. Sampai saat ini, Tuberkulosis (TBC) masih menjadi tantangan besar untuk pemerintah Indonesia. Untuk kepentingan diagnosis dengan cara pemeriksaan sputum mikroskopis langsung, terduga pasien TB diperiksa contoh uji sputum dengan pewarnaan Ziehl Neelsen. Namun, di beberapa daerah di Indonesia, jauhnya fasilitas kesehatan terkadang mengharuskan sampel BTA mengalami penundaan pemeriksaan karena melalui proses pengiriman terlebih dahulu. Tujuan penelitian ini adalah untuk mengetahui adanya pengaruh perbedaan suhu dan lama penyimpanan sputum terhadap jumlah Bakteri Tahan Asam. Desain penelitian ini adalah desain penelitian eksperimental laboratorium dengan rancangan acak lengkap (RAL). Penelitian dilakukan dengan 5 perlakuan yaitu penyimpanan selama 0 jam, 24 jam pada suhu 2-8oC, 24 jam pada suhu 25oC, 48 jam pada suhu 2-8oC dan 48 jam pada suhu 25oC. Berdasarkan pengamatan mikroskopis dan pengolahan data, diperoleh hasil p value > 0,05 maka Ho diterima dan Ha ditolak. Dari hasil tersebut dapat disimpulkan bahwa tidak terdapat pengaruh dari perbedaan suhu dan lama penyimpanan terhadap jumlah BTA +. Tuberculosis is a direct infectious disease caused by the TB bacteria, named Myobacterium tuberculosis. Until now, Tuberculosis (TBC) is still a big challenge for the Indonesian government. For the purpose of diagnosis by direct microscopic examination of sputum, suspected TB patients are examined for sputum samples with Ziehl Neelsen staining. However, in some areas in Indonesia, the distance from health facilities sometimes requires that Acid-Fast Bacilli samples undergo a delay in examination because they go through the delivery process first. Therefore, the purpose of this study was to determine the effect of differences in temperature and storage time on the amount of Acid-Fast Bacilli +. The design of this study is a laboratory experimental research design with a completely randomized design. The study was conducted with 5 treatments, that stored for 0 hours, 24 hours at a temperature of 2-8oC, 24 hours at a temperature of 25oC, 48 hours at a temperature of 2-8oC and 48 hours at a temperature of 25oC. After microscopic observation and data processing, the results obtained p value > 0.05, then Ho is accepted and Ha is rejected. From these results it can be concluded that there is no effect of differences in temperature and storage time on the amount of acid-fast bacilli +.
Co-Authors Abdullah Burhan Yuniarta Adelio Szaky Putra Indito Afita Indah Puspitasari Afrilia Rahmawati Ajeng Viona Putri Anjarani Alifa Purwahari Putri Ardy Prian Nirwana Didik Wahyudi Dwi Handayani Dyah Ayu Novita Sari Farmasiriana Deli Tantias Hari Saktiningsih Julita Niassinta Karisma PuriMahaliya Marcelina Wandan Wisdyafanny Nur Mutianingsih Rizky Fadzillah Sungkar Sevy Astriana Tri Harningsih Yordha Maharani Wahono Putri