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Optimizing artificial insemination on swamp buffalo (Bubalus bubalis) through synchronization of estrus and ovulation Sianturi, Riasari Gail; Purwantara, B; Supriatna, I; ., Amrozi; Situmorang, P
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 2 (2012)
Publisher : Indonesian Animal Sciences Society

Artificial insemination (AI) program in swamp buffalo will be more efficient by implementing synchronization of estrus and ovulation. By synchronizing of ovulation, AI can be done at a fixed time schedule without concerning to estrus detection. Gonadotropin Releasing Hormone (GnRH) and human chorionic gonadotropin (hCG) have been used in protocols of estrus synchronization to induce ovulation. A study of AI in swamp buffalo was conducted on 83 buffaloes to evaluate the impact of protocol of estrus synchronization on reproductive efficiency of swamp buffalo. The three protocols used were Ovsynch (GnRH-PGF2Î±-GnRH-AI), convensional (PGF2a-PGF2a-AI) and Select-Synch (GnRH-PGF2a-AI). Inducing of ovulation were done by administration of GnRH or hCG after prostaglandin (PGF2Î±) injection. AI was done at 18 and 24 hour after the second GnRH injection (66 hours and 72 hours after PGF2Î± injection) for Ovsynch method and 72 hours after the last PGF2Î± injection for convensional and Select-Synch methods. Parameters observed were percentage of estrus and pregnancy from the three estrus synchronization protocols and the differences were analysed by statistics. All of buffaloes (100%) in the three synchronization protocols showed estrus behavior prior to AI. The percentage of pregnancy was 64.71; 77.14 and 83.87% for the Ovsynch, convensional and Select-synch respectively and there was no significantly different (P > 0.05) among the three protocols. hCG administration after the last PGF2Î± also did not affect pregnancy rate, ie: 76.47 vs 77.78% (with hCG vs without hCG) for the convensional and 88.24 vs 78.57% for the Select-Synch. It is concluded that the synchronization of estrus protocols in this study can synchronize the estrous and ovulation and AI can be done in a fixed-timed and could reach better pregnancy rate of swamp buffalo. Key Words: Swamp Buffalo, Synchronization, Estrus, Ovulation, AI

Effect of glutathione and bovine seminal plasma in lactose extender on viability of swamp buffalo frozen semen Sianturi, Riasari Gail; Purwantara, B; Supriatna, I; ., Amrozi; Situmorang, P
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 3 (2012)
Publisher : Indonesian Animal Sciences Society

The aim of the study was to investigate the effect on viability of frozen swamp buffalo semen of glutathione and bovine seminal plasma in lactose extender. Semen from two swamp buffalo bulls was collected twice weekly using an artificial vagina. Pooled, good-quality fresh semen was divided into three parts and centrifuged at 3,000 rpm for 15 minutes in preparation for three treatments-substitution of buffalo seminal plasma with zero, 50 or 100% bovine seminal plasma (BS0, BS50 and BS100, respectively). Each semen aliquot was then divided in two parts, on which was diluted with lactose extender containing 1 mM glutathione (GSH) and the other diluted with lactose extender without GSH (0 mM GSH). Extended semen from all six treatments was cooled to 5oC and then frozen in 0.25 ml straws.Â Mean motility percentages 0 and 30 minutes post thaw (PTM 0â² and 30â²) with GSH were 38.33 and 34.29%, significantly higher (P < 0.05) than treatments without GSH (31.67 and 25.95%). PTM 0â² and 30â² were also higher (P < 0.05) with no substitution of bovine seminal plasma (BS0) than when buffalo seminal plasma was replaced with bovine seminal plasma at either 50 or 100%. Averages were 40.00 vs 34.46 and 30.54% (BS0 vs BS50 and BS100) at thawing and 36.96 vs 28.36 and 25.36% 30 minutes post-thaw. Mean percentages of live sperm (LD), intact plasma membrane (MPU) and intact acrosomal membrane (TAU) at thawing were not significantly different with or without addition of GSH. However,at 30â² post thawing, TAU and MPU were significantly higher in GSH treatments than inthose without GSH:Â 61.50 vs. 58.19% (MPU) and 59.81 vs. 57.38% (TAU). Mean percentages of LD, TAU and MPU 30â²post thawing were higher with no substitution ofbuffalo seminal plasma (BS0) (P < 0.05) than to BS50 and BS100 treatments. In conclusion, the addition of glutathione (GSH) improved the quality of frozen swamp buffalo semen, but the partial substitution of buffalo seminal plasma with bovine seminal plasmaprovided no beneficial effects. Key Words: Swamp buffalo, Semen, Antioxidant, Glutathione, Seminal plasma

Effect of glutathione and bovine seminal plasma in lactose extender on viability of swamp buffalo frozen semen Riasari Gail Sianturi; B Purwantara; I Supriatna; Amrozi .; P Situmorang
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 3 (2012): SEPTEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The aim of the study was to investigate the effect on viability of frozen swamp buffalo semen of glutathione and bovine seminal plasma in lactose extender. Semen from two swamp buffalo bulls was collected twice weekly using an artificial vagina. Pooled, good-quality fresh semen was divided into three parts and centrifuged at 3,000 rpm for 15 minutes in preparation for three treatments-substitution of buffalo seminal plasma with zero, 50 or 100% bovine seminal plasma (BS0, BS50 and BS100, respectively). Each semen aliquot was then divided in two parts, on which was diluted with lactose extender containing 1 mM glutathione (GSH) and the other diluted with lactose extender without GSH (0 mM GSH). Extended semen from all six treatments was cooled to 5oC and then frozen in 0.25 ml straws. Mean motility percentages 0 and 30 minutes post thaw (PTM 0′ and 30′) with GSH were 38.33 and 34.29%, significantly higher (P < 0.05) than treatments without GSH (31.67 and 25.95%). PTM 0′ and 30′ were also higher (P < 0.05) with no substitution of bovine seminal plasma (BS0) than when buffalo seminal plasma was replaced with bovine seminal plasma at either 50 or 100%. Averages were 40.00 vs 34.46 and 30.54% (BS0 vs BS50 and BS100) at thawing and 36.96 vs 28.36 and 25.36% 30 minutes post-thaw. Mean percentages of live sperm (LD), intact plasma membrane (MPU) and intact acrosomal membrane (TAU) at thawing were not significantly different with or without addition of GSH. However,at 30′ post thawing, TAU and MPU were significantly higher in GSH treatments than inthose without GSH: 61.50 vs. 58.19% (MPU) and 59.81 vs. 57.38% (TAU). Mean percentages of LD, TAU and MPU 30′post thawing were higher with no substitution ofbuffalo seminal plasma (BS0) (P < 0.05) than to BS50 and BS100 treatments. In conclusion, the addition of glutathione (GSH) improved the quality of frozen swamp buffalo semen, but the partial substitution of buffalo seminal plasma with bovine seminal plasmaprovided no beneficial effects. Key Words: Swamp buffalo, Semen, Antioxidant, Glutathione, Seminal plasma

Optimizing artificial insemination on swamp buffalo (Bubalus bubalis) through synchronization of estrus and ovulation Riasari Gail Sianturi; B Purwantara; I Supriatna; Amrozi .; P Situmorang
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 2 (2012): JUNE 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Artificial insemination (AI) program in swamp buffalo will be more efficient by implementing synchronization of estrus and ovulation. By synchronizing of ovulation, AI can be done at a fixed time schedule without concerning to estrus detection. Gonadotropin Releasing Hormone (GnRH) and human chorionic gonadotropin (hCG) have been used in protocols of estrus synchronization to induce ovulation. A study of AI in swamp buffalo was conducted on 83 buffaloes to evaluate the impact of protocol of estrus synchronization on reproductive efficiency of swamp buffalo. The three protocols used were Ovsynch (GnRH-PGF2α-GnRH-AI), convensional (PGF2a-PGF2a-AI) and Select-Synch (GnRH-PGF2a-AI). Inducing of ovulation were done by administration of GnRH or hCG after prostaglandin (PGF2α) injection. AI was done at 18 and 24 hour after the second GnRH injection (66 hours and 72 hours after PGF2α injection) for Ovsynch method and 72 hours after the last PGF2α injection for convensional and Select-Synch methods. Parameters observed were percentage of estrus and pregnancy from the three estrus synchronization protocols and the differences were analysed by statistics. All of buffaloes (100%) in the three synchronization protocols showed estrus behavior prior to AI. The percentage of pregnancy was 64.71; 77.14 and 83.87% for the Ovsynch, convensional and Select-synch respectively and there was no significantly different (P > 0.05) among the three protocols. hCG administration after the last PGF2α also did not affect pregnancy rate, ie: 76.47 vs 77.78% (with hCG vs without hCG) for the convensional and 88.24 vs 78.57% for the Select-Synch. It is concluded that the synchronization of estrus protocols in this study can synchronize the estrous and ovulation and AI can be done in a fixed-timed and could reach better pregnancy rate of swamp buffalo. Key Words: Swamp Buffalo, Synchronization, Estrus, Ovulation, AI

The Economic Efficiency of Buffalo Business (Bubalus Bubalis) With the Shepherd Supardi Rusdiana; Lisa Praharani; Riasari Gail Sianturi
SOCA: Jurnal Sosial Ekonomi Pertanian Vol 14 No 2 (2020): Vol.14.No 2, 2020
Publisher : Program Studi Agribisnis, Fakultas Pertanian, Universitas Udayana Jalan PB.Sudirman Denpasar, Bali, Indonesia. Telp: (0361) 223544 Email: soca@unud.ac.id

Buffalo has a very big role to the family economy of stockbreeders. But until now, buffalo business in Banten Province is still small, and has not led to commercial business. The research was conducted in Cibarani Village, Cisata District, Pandeglang Regency, Banten in 2016. This study used interview and questionnaire filling methods to the 24 buffalo breeders. By accidental sampling, sampling of respondents was found in the cage, house, paddy field and fields. Primary data were obtained from stockbreeders and secondary data were obtained from the local institution. Primary and secondary data were analyzed descriptively, quantitatively, t-test analysis and economic analysis. The aim of this research was to determine the economic efficiency of buffalo business (bubalus bubalis) with the shepherds in stockbreeders. The results showed that, the factors that influenced the increased economic efficiency of buffalo business in breeders had a significant effect on (P <0.05) to the breeders profits variable. The female buffalo business by breeding, breeders' profits was Rp.7.889.000 / year, with R / C 1.02 higher. The male buffalo business by fattened up, breeders’ profits amounting to Rp.7.575.000 with R / C of 1.05 lower. The buffalo business in breeders by shepherded, through male fattened up or breeding, was economically worth to try.

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