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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Ternak dan Veteriner Animal Production : Indonesian Journal of Animal Production Policy Brief Pertanian, Kelautan, dan Biosains Tropika

I Supriatna

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Author-ID : 393871

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Social Sciences Veterinary

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Viability of bovine demi embryo after splitting of fresh and frozen thawed embryo derived from in vitro embryo production Imron, M; Boediono, A; Supriatna, I
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

In vivo embryo production was limited by number of donor, wide variability respond due to superovulation program and also immunoactifity of superovulation hormone (FSH). Splitting technology could be an alternative to increase the number of transferrable embryos into recipien cows. Splitting is done with cutting embryo becoming two equal pieces (called demi embrio) base on ICM orientation.Â The objective of this research was to determine the viability of demi embryo obtained from embryo splitting of fresh and frozen thawed embryo. The results showed that demi embryos which performed blastocoel reexpansion 3 hours after embryo splitting using fresh and frozen thawed embryos were 76.9 and 76.2% respectively. Base on existention of inner cell mass (ICM), the number of demi embryos developed with ICM from fresh and frozen thawed embryos were not significantly different (90.6 and 85.7% respectively. The cell number of demi embryo from fresh embryos splitting was not different compared with those from frozen thawed embryos (36.1 and 35.9 respectively). These finding indicated that embryo splitting can be applied to frozen thawed embryos with certain condition as well as fresh embryos. Key Words: In Vitro Embryo, Splitting, Demi Embryo, Cell Number

Stallion semen cryopreservation using different cryoprotective agents on the skim milk trehalosa extender Arifiantini, R.I; Supriatna, I
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Cryoprotective agents (CPAs), protect the sperm during cryopreservation. There are two general classes of CPAs, at first penetrating cryoprotectants, these pass through the sperm membrane and act both intracellular and extracellularly, and the second non-penetrating cryoprotectants, these act only extracellularly. The objective of this study was to evaluate the addition of different CPAs namely glycerol (G), combination of ethylene glycol with glycerol (EG) and dimethilformamide (DMF) using skim milk trehalosa extender. The semen collected from 3 stallions using artificial vagina twice aweeks. The semen was evaluated, centrifugated and diluted in skim milk extender supplemented with 50 mM trehalose and three different CPAs with the concentration of sperm were 200x106 ml-1. Extended semen was then packed into minitub 0.3 ml and equilibrated at 4oC for 2 hours, freeze in the liquid nitrogen vapor for 10 minutes and stored in liquid nitrogen container -196oC. After 24 hours, the semen was thawed at 37oC for 30 second. Results of this experiment indicated that the percentage of motile and viable sperm in skim trehalosa extender using DMF and glycerol better than combination of ethylene glycol and glycerol. Â Key Words: Cryopreservation, Stallion Sperm, Cryoprotectant

Morphometric performances of Katingan cattle in Central Kalimantan Utomo, Bambang Ngadji; Noor, R.R; Sumantri, C; Supriatna, I; Gunardi, E.D
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 3 (2010)
Publisher : Indonesian Animal Sciences Society

The information of Katingan cattle as an indigenous cattle in Central Kalimantan is very limited, especially phenotypic characterization. A number of 72 heads, consist of 57 heads female and 15 heads male of Katingan cattle in adults category were used in this study. The samples were obtained from three sub districts in district of Katingan, those were Tewah Sanggalang Garing (Pendahara Village), Pulau Malan (Buntut Bali Village) and Katingan Tengah (Tumbang Lahang Village). The location were used as the variable of groups (subpopulation). This study was conducted to describe morphometric performance, which was analysed by discriminant and canonical to estimate the phylogenic relationship and to determine the discriminant variable among the group (subpopulation). The SAS package program version 6.0 and MEGA 2 program was used to analyze data. The observed parameters were body length, wither height, chest width, chest depth, chest circumference, hip heigth, hip width, head height, head length and head width. The chest depth and hip height of female cattle from group of Buntut Bali were lower (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The parameter of: chest width, hip width and body length of Buntut Bali groups were higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The average body weight of male Katingan cattle from Buntut Bali was higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups but there were not different (P > 0.05) on bodyweight of female Katingan cattle among location. The highest similarity value was Katingan cattle from group of Tumbang Lahang (92,86%) followed by group of Pendahara (40.91%) and group of Buntut Bali (38.89%), repectivelly. Katingan cattle from group of Pendahara and Tumbang Lahang had closest genetic distance (1.406), while the longest genetic distance (2.046) was between group of Buntut Bali and group of Tumbang Lahang. Genetic distance between group of Buntut Bali and Pendahara was 1.929. Katingan cattle from Pendahara and Tumbang Lahang within one cluster. Body length in canonical structure 2 can be used as discriminant variable. Key Words: Katingan Cattle, Morphometric, Genetic Distance, Discriminant Analysis, Canonical

Optimizing artificial insemination on swamp buffalo (Bubalus bubalis) through synchronization of estrus and ovulation Sianturi, Riasari Gail; Purwantara, B; Supriatna, I; ., Amrozi; Situmorang, P
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 2 (2012)
Publisher : Indonesian Animal Sciences Society

Artificial insemination (AI) program in swamp buffalo will be more efficient by implementing synchronization of estrus and ovulation. By synchronizing of ovulation, AI can be done at a fixed time schedule without concerning to estrus detection. Gonadotropin Releasing Hormone (GnRH) and human chorionic gonadotropin (hCG) have been used in protocols of estrus synchronization to induce ovulation. A study of AI in swamp buffalo was conducted on 83 buffaloes to evaluate the impact of protocol of estrus synchronization on reproductive efficiency of swamp buffalo. The three protocols used were Ovsynch (GnRH-PGF2Î±-GnRH-AI), convensional (PGF2a-PGF2a-AI) and Select-Synch (GnRH-PGF2a-AI). Inducing of ovulation were done by administration of GnRH or hCG after prostaglandin (PGF2Î±) injection. AI was done at 18 and 24 hour after the second GnRH injection (66 hours and 72 hours after PGF2Î± injection) for Ovsynch method and 72 hours after the last PGF2Î± injection for convensional and Select-Synch methods. Parameters observed were percentage of estrus and pregnancy from the three estrus synchronization protocols and the differences were analysed by statistics. All of buffaloes (100%) in the three synchronization protocols showed estrus behavior prior to AI. The percentage of pregnancy was 64.71; 77.14 and 83.87% for the Ovsynch, convensional and Select-synch respectively and there was no significantly different (P > 0.05) among the three protocols. hCG administration after the last PGF2Î± also did not affect pregnancy rate, ie: 76.47 vs 77.78% (with hCG vs without hCG) for the convensional and 88.24 vs 78.57% for the Select-Synch. It is concluded that the synchronization of estrus protocols in this study can synchronize the estrous and ovulation and AI can be done in a fixed-timed and could reach better pregnancy rate of swamp buffalo. Key Words: Swamp Buffalo, Synchronization, Estrus, Ovulation, AI

Effect of glutathione and bovine seminal plasma in lactose extender on viability of swamp buffalo frozen semen Sianturi, Riasari Gail; Purwantara, B; Supriatna, I; ., Amrozi; Situmorang, P
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 3 (2012)
Publisher : Indonesian Animal Sciences Society

The aim of the study was to investigate the effect on viability of frozen swamp buffalo semen of glutathione and bovine seminal plasma in lactose extender. Semen from two swamp buffalo bulls was collected twice weekly using an artificial vagina. Pooled, good-quality fresh semen was divided into three parts and centrifuged at 3,000 rpm for 15 minutes in preparation for three treatments-substitution of buffalo seminal plasma with zero, 50 or 100% bovine seminal plasma (BS0, BS50 and BS100, respectively). Each semen aliquot was then divided in two parts, on which was diluted with lactose extender containing 1 mM glutathione (GSH) and the other diluted with lactose extender without GSH (0 mM GSH). Extended semen from all six treatments was cooled to 5oC and then frozen in 0.25 ml straws.Â Mean motility percentages 0 and 30 minutes post thaw (PTM 0â² and 30â²) with GSH were 38.33 and 34.29%, significantly higher (P < 0.05) than treatments without GSH (31.67 and 25.95%). PTM 0â² and 30â² were also higher (P < 0.05) with no substitution of bovine seminal plasma (BS0) than when buffalo seminal plasma was replaced with bovine seminal plasma at either 50 or 100%. Averages were 40.00 vs 34.46 and 30.54% (BS0 vs BS50 and BS100) at thawing and 36.96 vs 28.36 and 25.36% 30 minutes post-thaw. Mean percentages of live sperm (LD), intact plasma membrane (MPU) and intact acrosomal membrane (TAU) at thawing were not significantly different with or without addition of GSH. However,at 30â² post thawing, TAU and MPU were significantly higher in GSH treatments than inthose without GSH:Â 61.50 vs. 58.19% (MPU) and 59.81 vs. 57.38% (TAU). Mean percentages of LD, TAU and MPU 30â²post thawing were higher with no substitution ofbuffalo seminal plasma (BS0) (P < 0.05) than to BS50 and BS100 treatments. In conclusion, the addition of glutathione (GSH) improved the quality of frozen swamp buffalo semen, but the partial substitution of buffalo seminal plasma with bovine seminal plasmaprovided no beneficial effects. Key Words: Swamp buffalo, Semen, Antioxidant, Glutathione, Seminal plasma

The qualitative variation of Katingan cattle Utomo, Bambang Ngaji; Noor, R.R.; Sumantri, C; Supriatna, I; Gunardi, E.D; Tiesnamurti, B
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 1 (2012)
Publisher : Indonesian Animal Sciences Society

The characterization is main activities in order to identify important trait of Katingan cattle. Information of its phenotypic characteristic is not available yet. The aim of the study is to describe the qualitative characteristic of Katingan cattle. Three location of the study were sub-district of Tewah Sanggalang Garing (Pendahara Village), sub-district of Pulau Malan (Buntut Bali Village), and sub-district of Katingan Tengah (Tumbang Lahang Village). The samples of Katingan cattle were taken as many as possible based on the field conditions to observe pattern of colour, growth of horn and gibbosity appear among two horns. General characteristic of Katingan cattle was various in colours of coat, having horns, humped and dewlop. The main characteristics were expressed at female of Katingan cattle. There were six variations of horn shape at female, however the growth of arching forwards was dominant (78.4%). Horn shape of male was generally grown upside (98.3%). Gibbosity was founded among horn only at female cattle (92.6%). There were 9 combinations of coat colour of female Katingan Cattle, those were brown reddish (27%), brown whitish (14.1%), brown like colour of Bali cattle (13.8%), black (12.5%), brown dull (9.6%), brown sorrel (9.3%), blackish (7.1%), white brownish (5.5%) and white grayish (4.5%). Male of Katingan cattle had eight colour combinations, those were brown whitish (14.8%), brown whitish and reddish (14.8%), brown reddish (13.1%), blackish (12.3%), brown whiteish with black withers (10.7%), brown sorrel (9.8%) and brown sorrel with black withers (7.8%). Based on qualitative analysis, the Katingan cattle had variations in coat of colour, growth of horn and the gibbosity. The variation of coat colour had potentially selection related to the value of cultural. Key Words: Katingan Cattle, Qualitative, Coat Colour, Horn, Gibbosity

Evaluation of genetic diversity of Katingan catlle and their genetic relationship with some other local catlle through DNA microsatellite analysis Ngaji Utomo, Bambang; Noor, R.R.; Sumantri, c.; Supriatna, I; Gurnadi, E.D.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 2 (2011)
Publisher : Indonesian Animal Sciences Society

Katingan cattle is one of Indonesian animal genetic resources located in Katingan District, Central Kalimantan. The Katingan cattle is predicted to be extint, therefore it is necessary to conserve the cattle. Breed characterization is the primary step in any conservation program. Characterization was done using 10 microsatellite markers to evaluate the genetic diversity of Katingan Cattle in three subpopulations and their genetic relationship with some other local cattle. A total number of 72 random whole blood of Katingan samples consisted of Pendahara (20 samples), Buntut Bali (20 samples), and Tumbang Lahang (32 samples) were used. The samples of Bali, PO, and Limousine Cattle were 11, 6, and 3 respectivelly. The number of 136 aleles were found with the variation from 9 aleles (ILSTS089) to 18 aleles (ILSTS013) and the average of the alele was 13.6 aleles per locus. A number of alel from Tumbang Lahang was higher (10.8 aleles) than Pendahara (10.4 aleles) and Buntut Bali subpopulation (7.3 aleles). Some loci produced polymorphic diagnostic aleles which varied from 1-7 types of allele. HEL013 and BM1818 had four aleles, while ILSTS026 and ILSTS089 had five and six aleles respectively. ILSTS029 and ILSTS036 had seven aleles. The diagnostic aleles were also found in Tumbang Lahang subpopulastion, as well as in Pendahara, and Buntut Bali. Heterozigositas values of Pendahara, Buntut Bali, and Tumbang Lahang subpopulation were 0.454, 0.478, and 0.529 respectively. While the average of heterozigositas (Ä¤) was 0.492. Subpopulation of Tumbang Lahang was closer genetically to Pendahara (0.169) than Buntut Bali (0.173) and also the subpopulation was closer genetically to PO cattle (0.259) when compared to Buntut Bali (0.311) and Pendahara (0.329). The population of Katingan cattle was within one kluster with PO Cattle. Key Words: Katingan Cattle, Microsatellite, Genetic Diversity

Integrity of swamp buffalo sperm on a variety of semen freezing process ., Herdis; Purwantara, B; Supriatna, I; Putu, I.G
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 1 (1999)
Publisher : Indonesian Animal Sciences Society

Sperm of swamp buffalo bulls is easily damaged during freezing process. Acrosomal intact and plasma membrane intact is important factors in fertilization process. This experiment was aimed to study the effect of freezing method on sperm integrity. The result of experiment indicated that the mean of intact acrosomal and the intact plasma membrane for 4 hours of equilibration (52.24 Â± 3.70% and 54.34 Â± 4.80%) was significant higher (P<0.05) than 2 hours of equilibration (39.00 Â± 3.32% and 43.44 Â± 4.91%) but was not significantly difference (P>0.05) with 6 hours of equilibration (47.92 Â± 4.51% and 51.58 Â± 4.25%). There were not significance difference between one step and two step of glycerolization. The best sperm integrity was resulted by freezing method with 4 hours of equilibration and two steps glicerolization. Â Key words : Swamp buffalo bulls, sperm integrity, freezing process

Optimalization of Equilibration and Thawing Methode on Freezing Process of Garut Ram Semen (Ovis aries) Herdis, Herdis; Toelihere, MR; Supriatna, I; Purwantara, B; Adikara, RTS
ANIMAL PRODUCTION Vol 7, No 2 (2005): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.047 KB)

Generally, the problem of semen freezing processÂ isÂ cold shock effect and intracelullar change due to water release which isÂ related toÂ ice crystal formation. One factor to solve the problem is finding out optimal equilibration time and thawing method so there is only a littleÂ damage spermatozoa during freezing process.Â The research has been doneÂ to exploreÂ theÂ influence ofÂ equilibration time and thawing methodeÂ onÂ frozen semen quality of garut rams. The results shown that the mean of percentages of progressive motile sperm, percentages of viable sperm, percentages of plasma membrane and acrosomal intact on four hours equilibration (52.50% ;Â 62.33%; 57.17% and 56.42%) Â were not significantly differentÂ (P>0.05) from five hours equilibration (52.27%;Â 63.67%;Â 56.92% and 57.58 %)Â and six hours equilibration (54.17%; 61.00%; 59.42% and 58.58%) respectively.Â The percentages of progressive motile sperm, percentages of viable sperm,Â Â percentages of plasma membrane andÂ acrosomal intact on the thawing methodÂ on the temperature of 370CÂ for 30 seconds (53.33% ;Â 62.39%; 57.94% dan 58.61) were not significantly differentÂ (P>0.05) from the thawing methodeÂ on the temperature of 250C for 45 seconds (52.22% ; 62.28%; 57.72% dan 56.44) respectively.Â The conclusion shown thatÂ the treatment of four hours equilibration, five hours equilibration,Â Â six hours equilibration and alsoÂ the treatment of thawing methodÂ on the temperature of 370CÂ Â for 30 seconds and the thawing methodeÂ on the temperature of 250CÂ Â for 45 seconds do not have effect on garut ram freezing semen quality. (Animal Production 7(2): 74-80 (2005) KeyÂ Words : Equilibration, Thawing, Semen, Garut ram

Determination of Equilibration Time of Stallionâ Semen Freezing with Extender Skim Milk Arifiantini, RI; Supriatna, I; Samsurizal, Samsurizal
ANIMAL PRODUCTION Vol 9, No 3 (2007): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.273 KB)

The objectives of this experiment were to determine the best equilibration time with extender skim milk in order to maintain the quality of frozen stallion semen.Â The semen was collected from three 5-8 years old stallions using artificial vagina.Â The semen characteristics and quality were evaluated macro- and microscopically and extended with skim milk extender (1:1). Â Â The semen was centrifuged at 3000 rpm for 15 minutes.Â Supernatant was removed and the pellet was re-extended with skim milk extender with 5% glycerol. Â The extended semen then packed in mini straw (0.5 mL), equilibrated at 5oCÂ for 1 hr (E1), 2 hrs (E2) and 3 hrs (E3), frozen in the liquid nitrogen (N2) vapor for 15 minutes and then stored in liquid N2 containerfor 24 hrs.Â The straw than thawed at 37oC for 30 seconds.Â Â The percentages of sperm motility and viability were observed.Â The result of this research showed that there were no significant differences Â betweenÂ equilibration at 1, 2 and 3 hrs on the percentages of Â sperm motility with the average of post thawed motility wasÂ Â 23.60 Â± 7.10%.Â Â The equilibration time affected the sperm viability (P<0.05) in all stallions, with the percentages of viable sperm between 38.10 % and 46.50%. (Animal Production 9(2): 145-152 (2007)Â Key Words: Equilibration, frozen semen, stallion

Co-Authors A Boediono Aminah Aminah Amrozi - B Purwantara B Purwantara B Tiesnamurti Bambang Ngadji Utomo Bambang Ngaji Utomo Bambang Ngaji Utomo BP Pardede C Sumantri C Sumantri C. Sumantri C. SUMANTRI E.D Gunardi E.D. Gurnadi Herdis . herdis herdis I Arifiantini I.G Putu M Agil M Imron MR Toelihere, MR NWK Karja P Situmorang R.I Arifiantini R.R Noor R.R. Noor R.R. Noor RI Arifiantini RI Arifiantini, RI Riasari Gail Sianturi RTS Adikara Samsurizal Samsurizal

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