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All Journal JSFK (Jurnal Sains Farmasi & Klinis)
Ronny Martien
Bagian Farmasetika, Universitas Gadjah Mada
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Validasi Penetapan Kadar Isolat Andrografolid dari Tanaman Sambiloto (Andrographis paniculata Nees) Menggunakan HPLC Yandi Syukri; Agung Endro Nugroho; Ronny Martien; Endang Lukitaningsih
Jurnal Sains Farmasi & Klinis Vol 2, No 1 (2015): J Sains Farm Klin 2(1), November 2015
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (572.282 KB) | DOI: 10.29208/jsfk.2015.2.1.42

Abstract

The aim of study was to develop quantitative analysis of isolated andrographolide from Andrographis paniculata and different solvent for prelimenary studies to preperation Self Nano Emulsifying Drug Delivery System (SNEDDS) using HPLC. The separation was acquired on Sunfire C18 column with an isocratic mixture of methanol and water at a ratio of 6:4, v/v as a mobile phase. The method to determine the content of isolated andrographolide showed an adequate precision, with a RSD smaller than 1%. The accuracy was analyzed by adding the standard andrographolide, and good recovery values were obtained for all concentrations used. The HPLC method developed in this study showed specificity and selectivity with linearity in the working range and good precision and accuracy, making it very suitable for the quantification of isolated andrographolide. Compared to the standard, the purity of the isolated andrographolide was 95.74 ± 0.29 %. Prelimenary study to determined the highest solubility of isolated andrographolide in oil, surfactant and co-surfactant phases for preperation of SNEDDS were obtained 1.226 ± 0.009 of Capryol-90, 2.965 ± 0.014 of tween 20, and  6.074 ± 0.101 mg mL-1 of PEG 400, respectively. Conclusion, this method suitable used to determination solublity of isolated andrographolide for preperation SNEDDS. 
Co-Authors Agung Endro Nugroho Endang Lukitaningsih Yandi Syukri