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Potential of Moringa (Moringa oleifera) leaf extract to inhibit the growth of pathogenic bacteria Edwardsiella tarda Farid Mukhtar Riyadi; Arief Prajitno; Mohamad Fadjar; Arif Syaifurrisal; Annisa Isti Fauziyyah
Journal of Aquaculture and Fish Health Vol. 10 No. 3 (2021): JAFH Vol. 10 No. 3 September 2021
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v10i3.25057

This study analyzed the antibacterial activity of Moringa oliefera leaf extract against the growth of Edwardsiella tarda bacteria. This study aims to determine the antibacterial activity of Moringa leaf extract (M. oliefera) against the growth of E.tarda bacteria. Inhibition testing is done by diffusion method (disc test). The disc test used five variations of concentration of 75 mg/L, 150 mg/L, 225 mg/L, 300 mg/L, and 375 mg/L on TSA (Tryptone Soya Agar) media and incubated for 2x24 hours. As a positive control, an antibiotic in the form of chloramphenicol was used. (5 mg/L) Moreover, distilled water was used as a negative control.Moringa leaf extract (M. oliefera) contains natural active compounds, bacteriostatic antibacterials, due to decreased bacterial growth after 48 hours of incubation. The highest inhibition diameter of E. tarda was 12.95 mm at a concentration of 375 mg/L after 24 hours incubation and decreased by 11.02 mm after 48 hours incubation. The highest inhibitory effectiveness was at a concentration of 375 mg/L with an effectiveness of 58.8%, while the effectiveness of the decrease was 48.1% after 48 hours of incubation.

Phytochemical Screening and Antimicrobial Activity of Roselle (Hibiscus sabdariffa L.) Flower Extract Against Aeromonas hydrophila Sitti Khairul Bariyyah; Arief Prajitno; Ating Yuniarti
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Medicinal plants as an antimicrobial agent may provide an alternative way to replace the use of antibiotics to control disease agents in aquaculture. Roselle flowers (Hibiscus sabdariffa L.) has been used in many sectors as a source of functional food, natural coloring agents as well as antimicrobial agents. The objectives of this study were to evaluate the phytochemical compound in methanolic extract of roselle flower and their antimicrobial activity against Aeromonas hydrophila. The phytochemical composition of roselle flower was evaluated using phytochemical screening and FTIR. While the antimicrobial activity was performed by using the disc diffusion agar and co-culture with A. hydrophila. The results of phytochemical screening confirmed the presence of alkaloids, flavonoids, saponins, steroids, triterpenoids, and tannins. The results of FTIR revealed that Roselle flower extract had the main phenolic compounds. The result of disc diffusion and co-culture method indicated that the roselle flower extract had antibacterial activity against A. hydrophila. This antibacterial activity depended on the concentration applied.Keywords: antibiotics, Co-culture, Disk diffusion, flavonoid, Hibiscus sp.

Phytochemicals and The Ability of Plantago major Linn. Extract to Inhibit The growth of Aeromonas hydrophila Annisa Farhana Dewi; Arief Prajitno; Ating Yuniarti
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Aeromonas hydrophila is a type of gram-negative bacteria that can harm humans and major animals that have poikilotherm properties such as fish and shrimp. These bacteria can cause haemorrhages in fish until death and causing fish farming production to failure. The use of medicinal plants has been trusted by people all over the world to overcome various disease problems, one of which is a disease caused by bacteria. Plantago major L. is known that able to inhibit the growth of both gram-positive and gram-negative bacteria. The purposes of this study were to identify the extracted compounds contained in Plantago major L., using FTIR and UV-VIS spectrophotometer and to evaluate antibacterial activity against A. hydrophila. The results of this study showed that crude extracts of Plantago major L. contained polar compounds such as phenols, flavonoids, saponins, and tannins. The presence of phenols and flavonoids was confirmed by the results of FTIR and UV-VIS. Furthermore, the crude extract of Plantago major L. significantly inhibited the growth of A. hydrophila (P <0.05).Keywords: Antibacterial, Co-culture, Extraction, FTIR, UV-VIS.

POTENSI PENGGUNAAN EKSTRAK DAUN SAGA (Abrus precatorius) sebagai ANTI BAKTERI Aeromonas hydrophila secara In Vitro Rahmalia Eka Kenitasari; Arief Prajitno; Arning Wilujeng; Genesia Gerrine
Jurnal Penelitian Pendidikan IPA Vol 9 No 9 (2023): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i9.3440

Many diseases are caused by bacterial pathogens, for example, Aeromonas hydrophila. One alternative that can be done to overcome this problem is to use saga leaves (Abrus precatorius) which have bioactive ingredients as antibacterial ingredients. The aims of this study were to determine the antibacterial activity of saga (A. precatorius) leaf extract, to study the character and structure of bacteria inhibited by saga (A. precatorius) leaf extract and to study the bioactive components contained in saga (A. precatorius) leaf extract. The results of the disc test showed that the average inhibition zone was 14,29 mm while the MIC test results showed that at the dose 1 mg/L capable of inhibiting bacterial growth (bacteriostatic) and dose 10 mg/L It has been able to kill bacteria (bactericidal). Crude extract of saga leaf extract (A. precatorius) affects the character and structure of A. hydrophila bacteria by damaging the cell walls and cell membranes of bacteria. The results of Infrared Spectrophotometry analysis of saga (A. precatorius) leaf extract and Ultra Violet Spectrophotometry analysis with 96% absorption ethanol were suspected to contain flavonoid compounds

POTENSI PENGGUNAAN EKSTRAK DAUN SAGA (Abrus precatorius) sebagai ANTI BAKTERI Aeromonas hydrophila secara In Vitro Rahmalia Eka Kenitasari; Arief Prajitno; Arning Wilujeng; Genesia Gerrine
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 9 (2023): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i9.3440

Many diseases are caused by bacterial pathogens, for example, Aeromonas hydrophila. One alternative that can be done to overcome this problem is to use saga leaves (Abrus precatorius) which have bioactive ingredients as antibacterial ingredients. The aims of this study were to determine the antibacterial activity of saga (A. precatorius) leaf extract, to study the character and structure of bacteria inhibited by saga (A. precatorius) leaf extract and to study the bioactive components contained in saga (A. precatorius) leaf extract. The results of the disc test showed that the average inhibition zone was 14,29 mm while the MIC test results showed that at the dose 1 mg/L capable of inhibiting bacterial growth (bacteriostatic) and dose 10 mg/L It has been able to kill bacteria (bactericidal). Crude extract of saga leaf extract (A. precatorius) affects the character and structure of A. hydrophila bacteria by damaging the cell walls and cell membranes of bacteria. The results of Infrared Spectrophotometry analysis of saga (A. precatorius) leaf extract and Ultra Violet Spectrophotometry analysis with 96% absorption ethanol were suspected to contain flavonoid compounds

Effectiveness of Administering Red Belt Leaf Extract (Piper Crocatum) Against Aeromonas Hydrophila Bacteria in Vitro Muhammad Jusril; Arief Prajitno; Mohamad Fadjar
Jurnal Penelitian Pendidikan IPA Vol 10 No 3 (2024): March
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i3.6074

Diseases caused by pathogenic bacteria such as Aeromonas hydrophila are a major health concern. However, the use of red betel leaves (P. crocatum) can serve as a potential solution to this problem due to their antibacterial properties. This research aimed to study the antibacterial activity of red betel leaf extract (P. crocatum), the structure and character of the bacteria inhibited by the extract, and the bioactive components present in the leaves. The results of the disc test showed that the average zone of inhibition was 14.28 mm, while the MIC test results revealed that a dose of 100 mg/L could inhibit bacterial growth (bacteriostatic). Moreover, the crude extract of red betel leaf (P. crocatum) damaged the cell walls and cell membranes of A. hydrophila bacteria, thereby affecting their character and structure. The UV-VIS analysis of red betel leaf extract (P. crocatum) and FTIR analysis with 96% ethanol absorption indicated the presence of flavonoids, saponins, tannins, and alkaloids. These findings suggest that red betel leaves can be used as a natural remedy to fight bacterial infections caused by pathogenic bacteria such as Aeromonas hydrophila

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