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All Journal The Journal of Experimental Life Sciences (JELS) Research Journal of Life Science Jurnal Penelitian Pendidikan IPA (JPPIPA)
Mohamad Fadjar
University of Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics

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Active Compounds on Squid (Loligo sp.) Ink Extract Powder as Immunostimulant Candidate to Against Shrimp Disease Rangga Idris Affandi; Mohamad Fadjar; Arning Wilujeng Ekawati
Research Journal of Life Science Vol 6, No 3 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (485.685 KB) | DOI: 10.21776/ub.rjls.2019.006.03.1

Abstract

The business of developing shrimp farming cannot be separated from the existence of disease. In shrimp farming health management, disease prevention strategies can using various methods, one of which is immunostimulant. One alternative immunostimulant source that can be used to improve the body defense system in shrimp is squid (Loligo sp.) ink extract powder who has antibacterial ability. This study aims to analyze the presence of active compound in squid ink extract powder can be used as an immunostimulant candidate to against shrimp disease. The method used in this research is descriptive explorative and experimental method. This research was conducted with several stages of squid ink extraction until it becomes powder. Identification of squid ink extract powder is using FTIR and LC-MS test. The results showed that the squid ink extract powder contained alkaloid and carboxylic acid from the FTIR test results. Based on LC-MS test results, it was found that squid ink extract powder contained betaine, cinnamic acid, and choline compounds with large amounts of content. Betaine, cinnamic acid, and choline has several biological activity as antibacterial, antioxidant, antiviral, antifungal, etc. so that it can be used as an immunostimulant against shrimp disease.
Antibacterial Ability of Rhizophora mucronata Leaf Extract Against Bacterial Infections of Edwardsiella tarda Ramanda Ahmad Rizal Rifa'i; Sri Andayani; Mohamad Fadjar
The Journal of Experimental Life Science Vol. 13 No. 1 (2023)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.01.02

Abstract

Edwardseilla tarda is a group of gram-negative bacteria with facultative anaerobic properties that can infect both cultivated and wild fish. Edwardseilla tarda infection causes considerable economic losses in the world. Rhizopora mucronata is a plant that is known to have the ability to produce secondary metabolites such as alkaloids and flavonoids. This study aims to determine the ability of R. mucronata to inhibit the growth of E. tarda as indicated by the presence of an inhibition zone and the absorbance value in the tube dilution test. The method used in this study is the disc diffusion test and the tube dilution test. The results of the phytochemical analysis showed the presence of flavonoid and alkaloid compounds in the crude extract of R. mucronata leaves. A concentration of 15.6 mg.L-1 showed a minimal inhibitory response in inhibiting the growth of E. tarda bacteria. In comparison, the concentration of 56 mg.L-1 showed the highest inhibitory response in inhibiting the growth of E. tarda bacteria. It indicates that the compounds contained in R. mucronata can be used as alternative ingredients in medicine to treat E. tarda bacterial infections. Keywords: Alkaloid, Antibacterial, E. tarda, Flavonoid, R. mucronata.
Pengaruh Ekstrak Kasar Daun Keji Beling (Strobilanthes crispus) terhadap Infeksi Bakteri Aeromonas hydrophila Secara In Vitro Genesia Gerrine; Arief Prajitno; Mohamad Fadjar; Rahmalia Eka Kenitasari
Jurnal Penelitian Pendidikan IPA Vol 9 No 5 (2023): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i5.3502

Abstract

Keji beling leaves (Strobilanthes crispus) can be the solution to cure the fish that infected by Aeromonas hydrophila because it contained active compounds. The purpose of this study was to determine the active compounds contained in the crude extract of keji beling leaves (S. crispus) and their ability to inhibit the growth of A. hydrophila. The tests which are used in this study were phytochemical test, MIC test, disk diffusion test, and SEM test. Phytochemicals analysis showed the presence of active compounds are flavonoid, alkaloid, tanin, and triterpenoid. The result of MIC test showed that concentration of 100 mg/l with a value of 0.389 was the lowest absorbance of the keji beling (S. crispus) crude leaves extract. The inhibition test used disk diffusin test with five concentrations of keji beling (S. crispus) crude leaves extract with three replications: 50 mg/l, 100 mg/l, 150 mg/l, 200 mg/l, and 250 mg/l, with the observation time of 24 hours post-incubation and 48 hours post-incubation. The concentration of 250 mg/l had the highest inhibition zone. The result of SEM analysis showed that keji beling (S. crispus) crude leaves extract can damage the cell walls of A. hydrophila and lysis the bacterial cells.
Phytoplankton Composition in White Shrimp (Litopenaeus vannamei) Pond Culture Infected White Feces Disease (WFD) Jefri Anjaini, S. Pi., M.P; Tohap Simangunsong; Mohamad Fadjar
Research Journal of Life Science Vol 10, No 1 (2023): IN PRESS
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

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Abstract

The aim of this study is to find out the composition and condition of phytoplankton in the white shrimp (L. vannamei) culture inffected by the white feces disease so that one of the indicators of the onset of the white feces disease can be known. This research uses descriptive methods. The observed parameters are the identification of species diversity, abundance, diversity index and dominance index of phytoplankton. The sampling was conducted at three locations, Tuban area, Situbondo area, Lamongan area. The results of the study showed observations in Lamongan area, the total abundance of phytoplankton was 473 cells/L.Situpondo areas abundantity of phytoplankton total was 633 cels/L and Tuban areas abundance was 887 cells/L. In Lamongan area, the diversity index was 1,009. In Situbondo areas diversity Index was 1,013. In Tuban area, diversity indexes were 1,082. In Lamongan area, a dominance index was 0.477. In Situpond area, it was obtained a domination index of 0.544. The genus that dominates the three ponds are Cyclotella and Navicula. At all three locations have high variety of species and abundance of the genus members of the filum Cyanophyta and Bacillariofhyta are detrimental, the genuses that are found are indicators of contaminated waters. The dominance of Cyanophyta (blue-green algae) is also an indicator of the White Feces Disease.
Effectiveness of Administering Red Belt Leaf Extract (Piper Crocatum) Against Aeromonas Hydrophila Bacteria in Vitro Muhammad Jusril; Arief Prajitno; Mohamad Fadjar
Jurnal Penelitian Pendidikan IPA Vol 10 No 3 (2024): March
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i3.6074

Abstract

Diseases caused by pathogenic bacteria such as Aeromonas hydrophila are a major health concern. However, the use of red betel leaves (P. crocatum) can serve as a potential solution to this problem due to their antibacterial properties. This research aimed to study the antibacterial activity of red betel leaf extract (P. crocatum), the structure and character of the bacteria inhibited by the extract, and the bioactive components present in the leaves. The results of the disc test showed that the average zone of inhibition was 14.28 mm, while the MIC test results revealed that a dose of 100 mg/L could inhibit bacterial growth (bacteriostatic). Moreover, the crude extract of red betel leaf (P. crocatum) damaged the cell walls and cell membranes of A. hydrophila bacteria, thereby affecting their character and structure. The UV-VIS analysis of red betel leaf extract (P. crocatum) and FTIR analysis with 96% ethanol absorption indicated the presence of flavonoids, saponins, tannins, and alkaloids. These findings suggest that red betel leaves can be used as a natural remedy to fight bacterial infections caused by pathogenic bacteria such as Aeromonas hydrophila
Co-Authors Arief Prajitno Arief Prajitno Arning Wilujeng Ekawati Genesia Gerrine Jefri Anjaini Muhammad Jusril Rahmalia Eka Kenitasari Ramanda Ahmad Rizal Rifa'i Rangga Idris Affandi Sri Andayani Tohap Simangunsong