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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Endar Marraskuranto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Effect of Agitation Speed and Cultivation Time on the Production of the Emestrin Produced by Emericella nidulans Marine Fungal muhammad nursid nursid; Martua Manulang; Joko Samiadji; Endar Marraskuranto
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 2 (2015): August 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i2.122

Abstract

Emestrin, an epipolythidioxopiperazine (EPT), is bioactive secondary metabolite produced by the marine fungus Emericella nidulans. Emestrin is potential to be developed as anticancer agent. Our present study investigated the effect of the agitation speed and cultivation time on the production of the mycelial biomass and emestrin in E. nidulans. The fungal was cultivated in malt extract broth (MEB) medium with varying agitation speeds of 0, 50, 100,150 rpm during 1,2,3 and 4 weeks of incubation at the temperature of 28 oC. Concentration of emestrin was determined by using high performed liquid chromatography (HPLC). The highest concentration of emestrin was found at static condition (0 rpm) cultivated for 1 week.
Cytotoxic Activity and Secondary Metabolite Characteristics of Sea Cucumber Actinopyga sp. Methanolic Extract Muhammad Nursid; Afrilia Putri Maharani; Riyanti Riyanti; Endar Marraskuranto
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 1 (2016): May 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1025.748 KB) | DOI: 10.15578/squalen.v11i1.193

Abstract

The objectives of this research were to assess cytotoxic activity and examine characteristic of sea cucumber Actinopyga sp. methanolic extract. Cytotoxicity assay was conducted using MTT method against WiDr (colon cancer) and T47D (breast cancer) cell lines. Actinopyga sp. methanolic extract was characterized by using phytochemical screening, Transform-Infrared (FT-IR) spectroscopy, dan  Liquid Chromatography Ion Trap Time of Flight Mass Spectrophotometer (LC-IT-ToF-MS).  The result showed that Actinopyga sp. methanolic extract inhibited Widr and T47D cell lines viability with the IC50 value of 55.93 and 87.55 µg/mL, respectively. Functional groups analysis showed the presence of hydroxyl, amine, carboxylic acid, nitrate, amide, sulphur, ester, and ether. LC-IT-ToF-MS analysis of crude extract showed that it containing many steroids compound.
FUCOIDAN FROM BROWN SEAWEED AND ITS BIOACTIVITY Ellya Sinurat; Endar Marraskuranto
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 3 (2012): December 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i3.8

Abstract

Fucoidan is a polysaccharide which  substantially consists of L-fucosa and ester sulphate group and is mainly contained in brown seaweed. For the past ten years, bioactivity studies of fucoidan has been conducted. Recently, fucoidan has been examined for its application in drugs. In a couple of years, fucoidan structure was succesfully identified and its bioactivity was revealed. Fucoidan exhibits various bioactivities such as anticoagulant, antioxidant, anticomplementary, anti-inflamation, gastric protector, and blood lipid level control. This review gives some brief progress in isolation and bioactivity study of fucoidan from brown seaweeds.
Influence of Salinity on Growth and Phycoerythrin Production of Rhodomonas salina Endar Marraskuranto; Tri J Raharjo; Rina S Kasiamdari; Tri R Nuringtyas
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 3 (2018): December 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i3.365

Abstract

Microalgae is a photoautotroph organism capable of producing various photosynthetic pigments with diverse beneficial properties. Rhodomonas salina, a Cryptophyte cell, contains only phycoerythrin as its phycobiliprotein pigment. The effects of salinity on growth and phycoerythrin concentration were investigated. Microalgae R. salina were grown in natural sea water with salinity of 33‰ and 50‰.The microalgae was batch-cultured in f/2 medium at light irradiation of 1100 lux, temperature of 24–26 oC, and photoperiode of 12 h : 12 h. The microalgae cell density was directly calculated using haemacytometer. The concentration of phycoerythrin was determined by spectrophotometric method. The cell density and phycoerythrin concentration were monitored every 4 days for 20 days of cell growth. Results showed that salinity did not affect significantly both on growth and phycoerythrin concentration extracted from R. salina biomass (p0.05; a = 0.05). At both salinity, maximum phycoerythrin concentration were reached on day 8. There was a positive correlation between cell density and phycoerythrin concentration from day 1 to day 8 of cell growth. Microalgae R. salina which was grown in natural seawater with salinity of 33‰ achieved the highest cell density of 8.4 x 105 cells/mL and the phycoerythrin concentration of 0.19 mg. 10-5 cell on day 8 of the culture. The highest phycoerythrin concentration was obtained on day 16 of the culture i.e 0.27 mg. 10-5 cell.Keywords: cell density, growth media, phycoerythrin, Rhodomonas salina, salinity
Co-Authors Afrilia Putri Maharani Ellya Sinurat Joko Samiadji Martua Manulang Muhammad Nursid muhammad nursid nursid Rina S Kasiamdari Riyanti Riyanti Tri J Raharjo Tri R Nuringtyas