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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Dwiyitno Dwiyitno
Research and Development Center for Marine and Fisheries Product Processing and Biotechnology
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Concentration and Characteristic of Floating Plastic Debris in Jakarta Bay: a Preliminary Study Dwiyitno Dwiyitno; Fairdiana Andayani; Umi Anissah; Hedi Indra Januar; Singgih Wibowo
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 15, No 3 (2020): December 2020
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v15i3.462

Abstract

Marine debris, especially plastic debris, is becoming global awareness, including in Indonesia, due to the possible harmful effects on the environment and humans. The present study is the first assessment of the floating plastic debris in Jakarta Bay based on an at-sea survey. The study aimed to overview the accumulation of plastic debris in different zones (west, middle, and east) of the bay as the impact of wet and dry seasons. The results showed that plastic debris was found in most sampling stations with a maximum concentration of 10,300 and 7,400 items/km2 in the wet and dry seasons, respectively. Based on the plastic types, polyethylene (PE), polypropylene (PP), and polystyrene (PS) were the main accumulated plastic debris in Jakarta Bay with a predominant macro size between 2.5 and 20 cm. According to the plastic applications, packaging and consumer products were the majority of plastic debris at concentrations of 257-1,280 items/km2 and 936-983 items/km2 in the dry and wet season respectively. The spatial distribution of plastic litter in the observed surface water was correlated to temporal monsoon seasons rather than to the distribution of pollution source from rivers around the Jakarta Metropolitan Area. Moreover, the higher plastic concentration was found in the rainy west monsoon compared to that of the dry east monsoon.
Method Comparison of DNA Isolation and Quantification for Fish and Seafood Authenticity Determination Dwiyitno Dwiyitno; Stefan Hoffman; Koen Parmentier; Chris Van Keer
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 3 (2018): December 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i3.370

Abstract

Fish and seafood products has been commonly targeted for fraudulent activities. For that reason, authentication of fish and seafood products is important to protect consumers from fraudulent and adulteration practices, as well as to implement traceability regulation. From the viewpoint of food safety, authenticity is beneficial to protect public from serious food poisoning incidents, such as due to ingestion of toxic species. Since DNA based identification depends on the nucleic acid polymerase chain reaction (PCR), the quantity and quality/purity of DNA will contribute significantly to the species authentication. In the present study, different DNA extraction and purification methods (3 classical methods and one commercial kit) were compared to produce the better isolated DNA for PCR amplification. Additionally, different methods for the estimation of DNA concentration and purity which is essential for PCR amplification efficiency were also evaluated. The result showed that classical DNA extraction methods (based on TNES-Urea) yielded a higher amount of DNA (11.30-323.60 ng/g tissue) in comparison to commercial kit/Wizard Promega (5.70-83.45 ng/g tissue). Based on the purity of DNA extract (A260/280), classical DNA extraction method produced relatively similar on DNA quality to the commercial kit (1.79-2.12). Interestingly, all classical methods produced DNA with A260/280 ratio of more than 2.00 on the blue mussel, in contrast with commercial kit. The commercial kit also produced better quality of DNA compared to the classical methods, showing the higher efficiency in PCR amplification. NanoDrop is promising as cheap, robust and safe UV-spectrophotometer method for DNA quantification, as well as the purity evaluation.Keywords: seafood authenticity, DNA isolation, polymerase chain reaction, NanoDrop, Picogreen
Emerging Pollutants from Industrial Emission and Their Health Hazards in Indonesian Seafood: A Review Dwiyitno Dwiyitno
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i3.399

Abstract

Emerging pollutants (EPs) or contaminants of emerging concern (CECs) have become global awareness since few decades ago, including in Indonesia. Intensive usage of industrial compounds has led to the massive emission to the environment and therefore their potential adverse effects may endanger aquatic organisms and human health. Based on the available literatures, organotins and flame retardants are two main groups of EPs from industrial emission identified in Indonesian seafood, as well as in those worldwide. However, concentration of both EPs group detected in Indonesian seafood is relatively low than that in developed countries and the majority of South East Asian countries. Toxicological studies revealed that EPs emitted from industrial activities have to be concern, as most of the EPs attributed to endocrine disrupting chemicals. Nevertheless, study on the exposure assessment of EPs in Indonesia is very limited. Even though, the concentrations of EPs in Indonesian seafood produce exposure below tolerable daily intake (TDI) to the local consumer, the long term exposures have to be aware due to their possible continues emission and elevating concentration such as shown by flame retardants. Governmental regulation, monitoring programs, and laboratory approach are among issues to be concern in addressing EPs in Indonesia, especially from industrial emission.
Co-Authors Chris Van Keer Fairdiana Andayani Hedi Indra Januar Koen Parmentier Singgih Wibowo Stefan Hoffman Umi Anissah