Hedi Indra Januar
Research and Development Center for Marine and Fisheries Product Processing and Biotechnology

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PERBANDINGAN BEBERAPA METODE ANALISIS HISTAMIN UNTUK PRODUK PERIKANAN Hedi Indra Januar
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 4, No 2 (2009): August 2009
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v4i2.150

Abstract

Studi ini  bertujuan  untuk  membandingkan  beberapa  metode dalam penentuan kadar amina  biogenik histamin. Diketahui, kadar histamin merupakan salah satu parameter yang penting  sebagai  standar  kualitas  produk  perikanan. Metode yang dibandingkan meliputi metode menggunakan spektrofluorometri, High Performance Liquid Chromatography (HPLC), Enzyme Linked Immunosorbent  Assay (ELISA), dan Capillary Electrophoresis/Capillary Zone Electrophoresis (CE/CZE). Hasil perbandingan menunjukkan bahwa baik dari sisi ketidakpastian yang mungkin ditimbulkan dari metodenya serta hasil uji kelayakan laboratorium di Eropa, metode HPLC derivatisasi post-kolom merupakan metode yang optimal saat ini untuk menentukan kadar histamin secara kuantitatif. Akan tetapi, untuk pertimbangan efisiensi waktu, maka studi ini mengusulkan bahwa penggabungan metode ELISA kualitatif dan HPLC kuantitatif sangat baik  dijadikan sebagai standar metode penentuan histamin di laboratorium pengujian produk perikanan.
CYTOTOXIC SATURATED FATTY ACIDS FROM THE INDONESIAN SEA CUCUMBER Holothuria sp. Hedi Indra Januar; Muhammad Nursid; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 9, No 1 (2014): May 2014
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v9i1.69

Abstract

Sea cucumbers are one of Indonesia’s marine organism with the potential to be developed as possible herbal medicines. In a preliminary study of cytotoxic activity of ethanol extracts of 14Indonesian sea cucumber species, the most active extract came from the Holothuria sp. Thecurrent research aimed to identify the major cytotoxic component in the sea cucumber Holothuriasp., that yielded the most active extract. The samples were collected from South Lampung beach.Isolation of the cytotoxic component was done employing liquid flash and preparative reversedphase (C 18) chromatography. Cytotoxic ity evalu atio n of fractio ns collected durin g thechromatography was conducted using the MCF-7 tumor cell line. Elucidation of the structure ofmost active isolate was done by NMR (Nuclear Magnetic Resonance) spectroscopy and GC-FID(Gas-Chromatography-Flame Ionisation Detector) analysis. The results of these analyses showedthe most active compound to be stearic acid; IC 50towards MCF-7 cells 10.32 ppm.
Chemical Composition and Fatty Acid Profile of Some Indonesian Sea Cucumbers Yusro Nuri Fawzya; Hedi Indra Januar; Rini Susilowati; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 1 (2015): May 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i1.118

Abstract

Indonesia has a great sea cucumber resource which is useful as functional food. Even though much information about the biopotency of sea cucumbers have been published, however, studies on nutrition value of Indonesian sea cucumbers are still beneficial due to the great diversity and uniqueness of each species. The present work was  intended to obtain information about chemical composition and fatty acid profile of 4 (four) types of sea cucumbers which are frequently found in Halmahera water, North Maluku (Molucca),  one  of  sea  cucumbers  producing area  in Indonesia. The samples coded as  H-03, H-04, H-05 and H-10, were identified as Bohadschia  argus, Holothuria  fuscogilva, Thelenota  ananas, and Actinophyga lecanora. All sea cucumbers showed high protein content, more than 60% (drybase/db), except for T. ananas which had the lowest protein content (48.26% db). As for fats, T. ananas showed the highest amount (2.35%db) with dominated saturated fatty acids (SFA). On the other side, A. lecanora showed higher value of PUFA compared to SFA and MUFA.  Palmitic acid (C16:0) was the most abundant SFA in the most samples with the amount of 0.49–4.9 mg/g sample. Among PUFA, eicosapentaenoic acid/EPA (C20:5n3) was detected, and eicosatrienoic acid (C20:3n6) was relatively higher than the others. The ratio of w6/w3 fatty acid was 1.2–3.2 showing that total w6 fatty acid was higher than total w3 fatty acid. However, this value is safe enough to protect against chronic and degenerative diseases.
Concentration and Characteristic of Floating Plastic Debris in Jakarta Bay: a Preliminary Study Dwiyitno Dwiyitno; Fairdiana Andayani; Umi Anissah; Hedi Indra Januar; Singgih Wibowo
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 15, No 3 (2020): December 2020
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v15i3.462

Abstract

Marine debris, especially plastic debris, is becoming global awareness, including in Indonesia, due to the possible harmful effects on the environment and humans. The present study is the first assessment of the floating plastic debris in Jakarta Bay based on an at-sea survey. The study aimed to overview the accumulation of plastic debris in different zones (west, middle, and east) of the bay as the impact of wet and dry seasons. The results showed that plastic debris was found in most sampling stations with a maximum concentration of 10,300 and 7,400 items/km2 in the wet and dry seasons, respectively. Based on the plastic types, polyethylene (PE), polypropylene (PP), and polystyrene (PS) were the main accumulated plastic debris in Jakarta Bay with a predominant macro size between 2.5 and 20 cm. According to the plastic applications, packaging and consumer products were the majority of plastic debris at concentrations of 257-1,280 items/km2 and 936-983 items/km2 in the dry and wet season respectively. The spatial distribution of plastic litter in the observed surface water was correlated to temporal monsoon seasons rather than to the distribution of pollution source from rivers around the Jakarta Metropolitan Area. Moreover, the higher plastic concentration was found in the rainy west monsoon compared to that of the dry east monsoon.