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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Kolaborasi Jurnal Pengabdian Masyarakat Mukhtabar: Journal of Medical Laboratory Technology Daarul Ilmi: Jurnal Pengabdian Kepada Masyarakat
Rivana Ariyadi
STIKes Muhammadiyah Ciamis
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Detection of rtxA Gene as a Biomarker of Seafood-Borne Pathogen Vibrio cholerae using In Silico PCR Assay Stalis Norma Ethica; Nur Hidayati; Hayatun Fuad; Chaerul Arham; Rivana Ariyadi; Ellyka Purwaningrum; Kazi Mohammad Zillur Rahman
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 15, No 2 (2020): August 2020
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v15i2.417

Abstract

Seafood-borne outbreaks caused by Vibrio cholerae have led to the increased need for food safety risk assessment of marine products. An in silico investigation about the potential of virulence gene of V. cholerae, rtxA, as a DNA biomarker of the toxigenic bacterium has been carried out. The aim of this study was to use the bacterial DNA biomarker sequence as a tool to facilitate early rapid detection of cholera infection. Five specific pairs of primers were designed from the rtxA open reading frame DNA of V. cholerae O1 biovar El Tor str. N16961 genomic DNA using Primer3Plus. Next, in silico Polymerase Chain Reaction (PCR) assay was carried out using the newly designed primers and 25 genomic DNA of vibrio spp. retrieved from the in silico database. One of the five designed pairs of primers, RtxAOF-RtxAOR: ‘5-CGCAAAACAGTTTCAGCCGA-3’ and 5’-AGGTTGGTCTTTTGTGGCCA-3’, could result in single DNA amplicon sized 518 bp only from V. cholerae species. No amplicon bands were produced from 17 other vibrio genomes studied using similar RtxAF-RtxAR primers. A further check showed that the amplicon was indeed part of the rtxA gene of V. cholerae. Based on this in silico study, rtxA gene appeared to be a DNA biomarker of V. cholerae, which is potential to facilitate rapid diagnosis of the virulence bacterium using in silico PCR assay.
Inisiasi Pos Pelayanan Terpadu Penyakit Tidak Menular (POSBINDU PTM) di SMA Negeri 2 Ciamis Siti Rohimah; Undang Ruhimat; Yoga Ginanjar; Rivana Ariyadi; Tika Sastraprawira
KOLABORASI JURNAL PENGABDIAN MASYARAKAT Vol 2 No 6 (2022): Kolaborasi: Jurnal Pengabdian Masyarakat
Publisher : Yayasan Inspirasi El Burhani

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (738.527 KB) | DOI: 10.56359/kolaborasi.v2i6.152

Abstract

Introduction: Evaluation of the Posbindu PTM program has been provided by the government, and various agencies have conducted several studies, literature review evaluation of Posbindu PTM provides an overview of input, process and output evaluations, output indicators show that the average coverage of Posbindu PTM visits is still low, the conclusion is that from each indicator the evaluation still found problems in its implementation. Objective: The purpose of this community service activity is screening, education and initiation of non-communicable disease control programs through POSBINDU PTM activities in the workplace.. Method: The implementation method used in this community service activity is an assessment using the interview method, physical examination and examination of supporting non-communicable disease risk factors. The activities are carried out in 3 stages: preparation, implementation, education and initiation. Result: Most of the participants had risky behavior, namely 91% did not eat enough vegetables and fruit, 89% lacked physical activity and risk factors for non-communicable diseases that had occurred were 78% bad body mass index, and 81% had bad cholesterol levels. Conclusion: there are still many risk factors for infectious diseases that have not been detected, especially in people who are still working, the initiation of PTM in the workplace is important to implement.
Identification Coliform Bacteria In Student Drinking Water In The Al-Mu'aawanah Boarding School, District Rajadesa, Ciamis District Undang Ruhimat; Rivana Ariyadi; Rahayu Nirmatul; Fatna Agnianisa Salsabila
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 1 (2023): Mukhtabar: Journal of Medical Laboratory Technology (April 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i1.207

Abstract

Background & Objective: Drinking water is a very important human need. Drinking water that is suitable for consumption must meet established standards, among others, according to the Minister of Health of the Republic of Indonesia No.492/Menkes/IV/2010. This study aims to determine the presence or absence of coliform bacteria in the drinking water of students using the MPN method. Methods: This type of research is descriptive. The sampling was carried out at the Al-Mu'aawanah Islamic boarding school. The results showed that the samples of drinking water for male and female students for microbiological parameters showed that the samples were positive for coliform bacteria. The sample for female students was 22/100 mL, and the sample for male students was 2/100 mL. Based on Permenkes No.472/Menkes/IV/2010, the maximum total concentration of coliform bacteria is 0/100 mL, which means that it is not in accordance with the standards of Permenkes No.472/Menkes/IV/2010, and the growth of faecal coliform bacteria was found in EMB media in both samples. namely the metallic green Escherichia coli bacteria, which indicates that the sample does not meet the microbiological requirements of drinking water.
Overview of Total Cholesterol Levels at Productive Age in the Imbanagara Health Center Working Area Intan Fandini; Undang Ruhimat; Rivana Ariyadi; Rahayu Nirmatul Mutmainah
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 2 (2023): Mukhtabar: Journal of Medical Laboratory Technology (October 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i2.368

Abstract

Background & Objective: Total cholesterol is the total amount of HDL, LDL, and VLDL cholesterol in each deciliter of blood. Risk factors that can affect total cholesterol levels are age, gender, sleep patterns, genetics, physical activity, and body mass index. This study aims to determine the description of total cholesterol levels in productive age in the Imbanagara Health Center Working Area. Method: This research method is descriptive research with a purposive sampling technique and involves 31 respondents the instrument used is a photometer. This research was conducted in the Imbanagara Health Center working area from May to June 2023. Result: The results showed that the total cholesterol levels of 31 respondents, were 25 people (81%) with normal total cholesterol levels, 5 people (16%) with high threshold total cholesterol levels, and 1 other person (3%) with high total cholesterol levels. Conclusion: It can be concluded that of the 31 respondents in the Imbanagara Health Center work area, the average has normal Total Cholesterol results.
Identification of Staphylococcus aureus Bacteria on the Palms of Visitors to Panumbangan Health Center Rivana Ariyadi; Putri Agnia Maulani; Undang Ruhimat; Rudy Hidana
Mukhtabar Journal of Medical Laboratory Technology Vol 1 No 2 (2023): Mukhtabar: Journal of Medical Laboratory Technology (October 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v1i2.376

Abstract

Background & Objective: Hand hygiene is one of the efforts to prevent and control the spread of bacteria that can cause infectious diseases. Nosocomial infections can be caused by gram-positive bacteria, such as Staphylococcus aureus which is the most dominant bacterium causing skin and soft tissue infections. The purpose of this study was to determine the presence of Staphylococcus aureus bacteria in the palms of visitors to the Panumbangan Health Center. Method: This study is a descriptive observational study with a simple random sampling technique conducted from May to June 2022.  Respondents in this study were 20 health center visitors. Sampling was carried out at the Panumbangan Health Center and the examination was carried out at the STIKes Muhammadiyah Ciamis Bacteriology Laboratory. The measurement methods used were gram staining, media culture, and biochemical tests. The data obtained from the examination results were presented descriptively in tabular form and explained in narrative. Result: The results showed that 6 samples (30%) identified Staphylococcus aureus, 11 samples (55%) Staphylococcus epidermidis, and 3 samples (15%) suspected Streptococcus sp.   Conclusion: It can be concluded that 30% of Staphylococcus aureus, 55% of Staphylococcus epidermidis, and 15% of Streptococcus sp. were identified.
Edukasi Zat Pewarna Pangan Berbahaya Di SMA Negeri 1 Sukadana Doni Setiawan; Rivana Ariyadi; Euis Tia Istianah; Aldi Febriansyah; Amanda Tyas Zaharani; Chintya Nur Aeni; Desry Delia Futri; Dinda Putri Stevani; Firdaus Nur Rohman; Hayfa Azkal Azkiya; levi Ayu Ramadhanty; Mitha Silvyana; Nailah Ghaissani Dwi Gustari; Nuraisyah Kurniawan; Putri Laksmi Margarethi; Risma Risma; Taopik Cahyadi
Daarul Ilmi: Jurnal Pengabdian Kepada Masyarakat Vol. 1 No. 2 (2023): Daarul Ilmi: Jurnal Pengabdian Kepada Masyarakat (Juli-Desember 2023)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/daipkm.v1i2.410

Abstract

Introduction: In general, food coloring can be obtained from natural dyes and safe synthetic food dyes. Natural dyes are sometimes considered less stable and less durable than synthetic food dyes. Although there are synthetic dyes that are safe for food, there are still people who use materials that are not suitable for use in food. The use of harmful food dyes must be monitored and recognized for their existence. Harmful food dyes that are commonly found are textile dyes such as Rhodamine B or methanyl yellow. Because it is not a proper food coloring, consumption of foods containing this ingredient can cause a series of health problems for those who consume it, especially in a relatively long period of time. This education aims to allow students to distinguish foods that use natural coloring agents and artificial coloring agents. The results of this activity students can find out the characteristics of foods that use harmful dyes and know the prevention that can be done. Objective: The purpose of this service is for SMAN 1 SUKADANA students to know the dangers of using hazardous coloring agents in food. Method: The method used is providing education by providing material to students, tests before and after material on the Use of Harmful Coloring Substances in Food. Result: Increased knowledge and attitudes of students are tested through pre-test and post-test. There has been an increase in knowledge in students about the dangers of using harmful dyes in food in general by 20%, so that the realization of the purpose of this service is expected to be able to distinguish food ingredients that contain harmful coloring agents in food and those that do not contain harmful coloring agents in food. Basically, knowledge and attitudes also depend on the high or low level of education of students. Therefore, the existence of this counseling can help students in adding insight into the dangers of using harmful dyes. The implementation of this activity can run well due to positive support in the implementation process, where support from various parties and there are no obstacles whatsoever. Conclusion: The socialization activity on the use of dangerous coloring substances in food to students at SMAN 1 Sukadana received a good response from the school and also students. With outreach activities regarding this, students will understand and be more careful in choosing food to avoid these chemicals.
Co-Authors Aldi Febriansyah Amanda Tyas Zaharani Chaerul Arham Chintya Nur Aeni Desry Delia Futri Dinda Putri Stevani Doni Setiawan Ellyka Purwaningrum Euis Tia Istianah Fatna Agnianisa Salsabila Firdaus Nur Rohman Hayatun Fuad Hayfa Azkal Azkiya Intan Fandini Kazi Mohammad Zillur Rahman levi Ayu Ramadhanty Mitha Silvyana Nailah Ghaissani Dwi Gustari Nur Hidayati Nuraisyah Kurniawan Putri Agnia Maulani Putri Laksmi Margarethi Rahayu Nirmatul Rahayu Nirmatul Mutmainah Risma Risma Rudy Hidana Siti Rohimah Stalis Norma Ethica Taopik Cahyadi Tika Sastraprawira Undang Ruhimat Yoga Ginanjar