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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Elvi Yetti
Laboratory of Biocatalyst and Fermentation Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Screening of Marine Bacteria Capable of Degrading Various Polyaromatic Hydrocarbons Elvi Yetti; Ahmad Thontowi; Yopi Y; Puspita Lisdiyanti
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 3 (2015): December 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i3.123

Abstract

This research aims to screen and to identify marine bacteria able to degrade Polyaromatic Hydrocarbons (PAHs). 7 bacterial isolates were screened for the ability to degrade 6 PAHs (phenanthrene, naphthalene, dibenzothiophene, fluorene, phenothiazine, and pyrene). This screening step was conducted by sublimation method, in which the color change or clear zone appearance was used as an qualitative indicator to show the capability of the selected isolate to degrade PAHs. Two bacterial isolates, designated as LBF-1-0102 and LBF1-0103, showed capability in degradating the PAHs tested. In particular, the isolate LBF-1-102 was potential in degrading phenanthrene, naphthalene, dibenzothiphene, fluorene, phenothiazine, and pyrene.The isolate LBF-1-0103  showed the ability in degrading 2 (two) PAHs, i.e. dibenzothiophene and  fluorene. Based on partial 16S rDNA analysis, LBF-1-0102 and LBF-1-0103 were identified as Pseudomonas balerica and Brachybacterium sp., respectively.
Co-Authors Ahmad Thontowi PUSPITA LISDIYANTI Yopi Y