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All Journal Journal of Medicine and Health Chimica et Natura Acta
Sylvia Soeng
Bagian Biologi, Fakultas Kedokteran, Universitas Kristen Maranatha
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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The Effect of Mangosteen Peel Extract on Sperm Motility and Sperm Count of Swiss Webster Mice Induced by Hard Physical Exercise Prawira, Ardi; Hasianna, Stella T.; Soeng, Sylvia
Journal of Medicine and Health Vol 1, No 1 (2015)
Publisher : Maranatha Christian University

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Abstract

High-intensity physical exercises can increase oxidative stress, causing damage to reproductive system. The purpose of this study was to determine the effect of mangosteen peel extract on sperm motility and  sperm count of  Swiss Webster mice induced by high-intensity physical exercise. This was a real experimental with complete randomized design. Twenty five  male Swiss Webster mice were divided into five groups (n = 5). Negative control (KN) was without any treatment, positive control (KP) was given  treadmill exercise only, the treatment groups (KM1, KM2, KM3) were given mangosteen peel extract orally 2, 4, 8 mg respectively as well as treadmill exercises. The treadmill exercises was conducted for 35 days. The sperm motility (mm/second) and the sperm count (per mm3) were assessed manually using Improved Neaubauer chamber. The result were analyzed using one-way ANOVA followed by Tukey HSD. The result showed high significant differences of sperm motility and sperm count between KM2 and KP, also between KM2 with  KM1 and  KM3 (p=0,000). The conclusion was mangosteen peel extract improved the sperm motility and sperm count of Swiss Webster mice induced by high-intensity physical exercise with 4 mg as the optimal dose. Keywords: mangosteen peel, sperm motility, sperm count
PENGUJIAN AKTIVITAS INHIBITOR LIPASE EKSTRAK ETANOL DAN HASIL FRAKSIONASI DARI KEDELAI DETAM 1 DAN DAUN JATI BELANDA Hidayat, Meilinah; Soeng, Sylvia; Prahastuti, Sijani
Chimica et Natura Acta Vol 2, No 1 (2014)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.86 KB) | DOI: 10.24198/cna.v2.n1.9146

Abstract

Salah satu pilihan pengelolaan obesitas adalah memperlambat absorbsi asam lemak dengan cara menginhibisi enzim lipase dalam saluran pencernaan. Inhibitor lipase pankreas dilaporkan telah berhasil dalam pengelolaan terapi obesitas. Senyawa aktif dalam Jati Belanda dan kedelai dapat berfungsi sebagai inhibitor lipase. Tujuan penelitian ini adalah mengetahui aktifitas lipase dari ekstrak etanol kedelai Detam 1 (EEKD), daun Jati Belanda (EEJB), kombinasi serta hasil fraksionasinya menggunakan metode standar dan modifikasi. Uji aktifitas lipase terhadap EEKD, EEJB, kombinasinya serta fraksi etil asetat, air dan n-heksana menggunakan kit Lipase Liquicolor (HUMAN®) dengan prinsip kolorimetri, menggunakan spektrofotometer 580 nm. Metode modifikasi menggunakan 96 well plate, microplate reader 630 nm. Penelitian ini menyimpulkan bahwa aktifitas inhibitor lipase tertinggi didapatkan pada sampel kombinasi EEKD:EEJB perbandingan 1:2, baik menggunakan metode standar maupun metode modifikasi; fraksi yang mengandung aktifitas inhibitor lipase paling tinggi adalah fraksi air kedelai Detam 1 (KD-1) dan air Jati Belanda (JB) pada 100 ppm, serta aktifitas lipase dalam fraksi air JB lebih tinggi dari fraksi air KD-1.
Co-Authors Ardi Prawira, Ardi MEILINAH HIDAYAT Sijani Prahastuti Stella T. Hasianna, Stella T.