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All Journal JURNAL FARMASIMED (JFM) Jurnal Pengmas Kestra (JPK)
Suventi Syafrina Ginting
Institut Kesehatan Medistra Lubuk Pakam
Health Professions Medicine & Pharmacology Nursing Public Health

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IDENTIFIKASI BAKTERI ESCHERICHIA COLI O157:H7 PADA FESES PENDERITA DIARE DENGAN METODE KULTUR DAN PCR Vincentia Ade Rizky; Sa’adah Siregar; Visensius Krisdianilo; Asvia Rahayu; Suventi Syafrina Ginting; Kartini .
Jurnal FARMASIMED (JFM) Vol 3 No 2 (2021): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i2.615

Abstract

Escherichia coli O157: H7 is the main cause of foodborne disease in several countries, one of which is diarrhea. Diarrheal disease is still a major problem in Indonesia that needs treatment and study from various aspects. The conventional method of laboratory examination such as culture is a method that is often carried out, but in making the diagnosis requires a long time, the number of samples is large, and the results are less accurate because contamination can occur. Another more accurate technique for detecting Escherichia coli O157: H7 is the PCR technique. This study aims to identify the Escherichia coli O157: H7 bacteria by culture method and PCR. The results showed that the culture method and PCR of 8 isolated samples 4 showed positive results for the bacterium Escherichia coli O157: H7. However, the PCR method is more selective and faster than the culture method.
SEMINAR PEMERIKSAAN DENGAN METODE REAL TIME POLYMERASE CHAIN REACTION ASSAY (RT-PCR) SEBAGAI TES CEPAT MYCOBACTERIUM TUBERCULOSIS DARI SAMPEL DAHAK PASIEN TUBERCULOSIS DI PUSKESMAS LUBUK PAKAM Suventi Syafrina Ginting; Visensius Krisdianilo
JURNAL PENGMAS KESTRA (JPK) Vol 1 No 2 (2021): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.558 KB) | DOI: 10.35451/jpk.v1i2.633

Abstract

Mycobacterium tuberculosis is known as a highly pathogenic bacterium. These bacteria are aerobic in shape like rods and have resistance to acids. These bacteria can cause tuberculosis (TB). TB disease has been widely known as a fairly high cause of death in the world. Tuberculosis causes the death of nearly one million women each year. Currently not a single country in the world has been free of tuberculosis. Data shows that Indonesia is the third largest contributor to tuberculosis cases in the world. Examination of mycobacterium tuberculosis bacteria that are routinely performed in hospitals or health centers is using microscopic diagnosis of acid-resistant basil (BTA). Recently there has been a quick test of Mycobacterium tuberculosis using the semi-quantitative Real Time Polymerase Chain Reaction Assay (RT-PCR) method that targets the rpoB gene in Mycobacterium tuberculosis, which can automatically process preparations by extraction of doxyribo nucleic acid (DNA) in catridge. Rt-PCR test results are very specific in detecting Mycobacterium tuberculosis. There are some results that are not detected with microscopic examination (BTA) can be detected by RT-PCR technique. However, overall there is no significant difference in results between microscopic methods and RT-PCR. As well as seminar participants can follow and understand the materials submitted and also the examination procedures carried out.
Co-Authors Asvia Rahayu Kartini . Sa’adah Siregar Vincentia Ade Rizky Visensius Krisdianilo