JURNAL FARMASIMED (JFM)
Vol 3 No 2 (2021): Jurnal Farmasimed (JFM)

IDENTIFIKASI BAKTERI ESCHERICHIA COLI O157:H7 PADA FESES PENDERITA DIARE DENGAN METODE KULTUR DAN PCR

Vincentia Ade Rizky (INKES MEDISTRA LUBUK PAKAM)
Sa’adah Siregar (Institut Kesehatan Medistra Lubuk Pakam)
Visensius Krisdianilo (Institut Kesehatan Medistra Lubuk Pakam)
Asvia Rahayu (Institut Kesehatan Medistra Lubuk Pakam)
Suventi Syafrina Ginting (Institut Kesehatan Medistra Lubuk Pakam)
Kartini . (Institut Kesehatan Medistra Lubuk Pakam)



Article Info

Publish Date
30 Apr 2021

Abstract

Escherichia coli O157: H7 is the main cause of foodborne disease in several countries, one of which is diarrhea. Diarrheal disease is still a major problem in Indonesia that needs treatment and study from various aspects. The conventional method of laboratory examination such as culture is a method that is often carried out, but in making the diagnosis requires a long time, the number of samples is large, and the results are less accurate because contamination can occur. Another more accurate technique for detecting Escherichia coli O157: H7 is the PCR technique. This study aims to identify the Escherichia coli O157: H7 bacteria by culture method and PCR. The results showed that the culture method and PCR of 8 isolated samples 4 showed positive results for the bacterium Escherichia coli O157: H7. However, the PCR method is more selective and faster than the culture method.

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Journal Info

Abbrev

JFM

Publisher

Subject

Medicine & Pharmacology

Description

Jurnal FARMASIMED (JFM) Volume I Nomor 1 Periode Mei - Oktober Tahun 2018. Jurnal ini memberikan ruang bagi akademisi, peneliti dan pengguna hasil penelitian dan pengabdian untuk mendiseminasikan, menginformasikan, mendiskusikan dan menggunakan hasil penelitian dan pengabdian sebagai upaya ...