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All Journal JURNAL FARMASIMED (JFM) Jurnal Pengmas Kestra (JPK)
Suventi Syafrina Ginting
Institut Kesehatan Medistra Lubuk Pakam
Health Professions Medicine & Pharmacology Nursing Public Health

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Journal : JURNAL FARMASIMED (JFM)

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IDENTIFIKASI BAKTERI ESCHERICHIA COLI O157:H7 PADA FESES PENDERITA DIARE DENGAN METODE KULTUR DAN PCR Vincentia Ade Rizky; Sa’adah Siregar; Visensius Krisdianilo; Asvia Rahayu; Suventi Syafrina Ginting; Kartini .
Jurnal FARMASIMED (JFM) Vol 3 No 2 (2021): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i2.615

Abstract

Escherichia coli O157: H7 is the main cause of foodborne disease in several countries, one of which is diarrhea. Diarrheal disease is still a major problem in Indonesia that needs treatment and study from various aspects. The conventional method of laboratory examination such as culture is a method that is often carried out, but in making the diagnosis requires a long time, the number of samples is large, and the results are less accurate because contamination can occur. Another more accurate technique for detecting Escherichia coli O157: H7 is the PCR technique. This study aims to identify the Escherichia coli O157: H7 bacteria by culture method and PCR. The results showed that the culture method and PCR of 8 isolated samples 4 showed positive results for the bacterium Escherichia coli O157: H7. However, the PCR method is more selective and faster than the culture method.
Co-Authors Asvia Rahayu Kartini . Sa’adah Siregar Vincentia Ade Rizky Visensius Krisdianilo