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Penta Suryaminarsih
Agrotechnology Study Program, Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran” Jawa Timur, Surabaya 60294, Indonesia
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Prospects of Biological Control of Green Ladybugs (Nezara viridula) Using Streptomyces spp. Laksamana Agadhia Raharjo; Penta Suryaminarsih; Dita Megasari
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1504

Abstract

Ecosystem damage due to the use of chemical pesticides increases every year, therefore alternative searches need to be known and developed. APH (Biological Control Agency) is a beneficial microbe that can control pest and disease populations. Streptomyces spp. Is a gram-positive soil bacterium that can be used against green ladybugs because Streptomyces spp. Able to produce chitinase enzymes that can break down chitin compounds in Exo – Skeleton Arthropod animals The purpose of this study was to determine the prospects for the use of Streptomyces spp. as APH of green shieldbugs.The description of this article includes: research results of Streptomyces spp., morphology, bio-ecology and bio-molecular green shieldbugs (Nezara viridula), as well as prospects for the use of Streptomyces spp. the results show that the use of Streptomyces spp. is able to control green ladybugs, because the chitinase enzyme produced can break down the chitin compound.The development of Streptomyces spp. has a lot of space, developments in the production of chitinase enzymes can be implemented and have clear potential.
Exploration and Identification of the Entomopathogenic Fungus Metarhizium anisopliae in Corn Plants in Sebandung Village, Sukorejo, Pasuruan Rateh Lailatul Risdiyanti; Wiludjeng Widayati; Penta Suryaminarsih
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur 2021
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2002

Abstract

Metarhizium anisopliae is a biological control agent (BCA) that have been developed to control plant pest in this disruption era. These fungi penetrate the insect body through its skin between the head and the thoraxs also in between the joint. The purpose of this research is to identify the characteristic of the fungi M. Anisopliae from the exploration in sebandung village, sub-district Sukorejo, district Pasuruan. Data analysis is done by using descriptive data. Step of the research involves (1) sample gathering from the soil in the cornfield that has been predicted to have BCA biodiversity, after that determined 5 sampling points diagonally, each sample contains 100 gr of soil from the depth of 10 -20 cm, (2) fungi baiting by using a mealworm (Tenebrio Molitor) as a bait, (3) M. Anisopliae isolate using PDA (Potato Dextrose Agar), (4) identification is conducted by macroscopic and microscopic. The identification result shows that the isolate has a white mycelium that forms into a mossy green spore, fine texture, easily spread, and insulated mycelium, straight conidiophore, hyalin, and branching, single-cell conidia, hyalin, round cylinder type, also white to the mossy green colony. Parasitism test results show that T.molitor larvae develop a hard body, have a murky color, covered in white hyphea till it transforms into moosy green spore with a fine texture and easily spread, the spore is the final form of the fungi propagule existence in the host as a survival phase in extreme condition.
The Presence of Soybean Plant Insects (Glycines Max L. Merril) in the Vegetative Phase with the Application Treatment of Streptomyces sp. and Trichoderma sp. Nugraha Iqbal Avrianto; Penta Suryaminarsih; Wiludjeng Widajati
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur 2021
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2005

Abstract

Soybean (Glycine max (L.) Merill) is one of the main food crops in the community. In East Java, productivity has declined due to various factors, including pest attacks. This study aims to determine the diversity of insects by administering a mixture of biological control agents (BSAs) between Streptomyces sp. and Trichoderma sp. as an entomopathogen applied to soybean plants in the vegetative phase. The soybean plants were planted in plastic bags on the ground of the Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran Jawa Timur. The aim of this study was to determine the effect of Streptomyces sp. and Trichoderma sp. the type and population of parasites. This study used a randomized two-way block design of 13 treatments repeated 3 times. Based on the observations, the total number of insects found in the experimental field of UPN veterans Jawa Timur was 2,360 individuals, made up of six orders of insects. The four orders are divided into 8 families and 8 species. Hemiptera species became the most dominant order (2202 individuals; 93%), Orthoptera (108 individuals; 5%), Lepidoptera (18 individuals; 1%) and Diptera (32 individuals; 1%) . The use of Streptomyces sp. and Trichoderma sp. the single comparison treatment is more effective than the combined comparison on the pest population. The main insects include Aphid spp, Bemisia tabaci and Nezera viridula.
Pest and Disease Control in Cavendish Banana Seedlings Resulting from Tissue Culture Indah Sari Dwi Agustin; Penta Suryaminarsih; Putranto Sasikirono; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur 2021
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2013

Abstract

Cavendis banana cultivation can use tissue culture as one of the developments of cultivation in the era of disruption. However, the results of tissue culture are very susceptible to attack by nuisance organisms during acclimatization to plant culture. The control carried out against the attack of plant-disturbing organisms in addition to using fungicides and insecticides, also applies preventive control. The purpose of this study was to determine an effective and smart way to control pests and diseases in tissue cultured Cavendish banana seedlings. Preventive control of pests and diseases using the method of thinning the seeds and soaking the seeds with fungicides. Data analysis was carried out using descriptive and parametric data. This study was conducted using a completely randomized design (CRD) with four control treatments. Each treatment was repeated 4 times. The control treatments carried out consist of: Control (A), preventive control (B), chemical control (C), and a combination of preventive and chemical control (D). The results of the control carried out showed that the combination of preventive and chemical control treatments gave significant results in inhibiting the attack of pests and diseases of Cavendish banana seedlings from tissue culture.
The Effectiveness of Vegetable Neem Leaf Pesticides Against Purple Spot Disease on Onion Plants in The Rain Season Serlia Nur Abifah; Penta Suryaminarsih; Juniawan
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3114

Abstract

Purple spot disease is one of the main diseases in leaf onion cultivation. One form of control is carried out by utilizing neem plants, especially the neem leaves which have the potential as vegetable pesticides (fungicides). This study aims to determine the effectiveness of neem leaf vegetable pesticide and to obtain the right concentration of neem leaf vegetable pesticide in controlling purple spot disease on a leaf in rainfall season. This study used an experimental method designed in a single factor Completely Randomized Design (CRD) using neem leaf pesticide consisting of three treatments (control, 10%, and 20%). The results showed that neem leaves at a concentration of 20% were effective as a vegetable pesticide to control purple spot disease on leaf onion. Neem leaf vegetable pesticides at a concentration of 20% were able to inhibit the increase in the area of attack symptoms and also inhibit the intensity of purple spot disease attacks on leaf onion by 13.84%. This is caused by the increase in concentration causing an increase in the content of the active ingredient in the substance which functions as a pesticide that is able to inhibit the spread of large quantities.
Co-Authors Dita Megasari Indah Sari Dwi Agustin Juniawan Laksamana Agadhia Raharjo Nugraha Iqbal Avrianto Putranto Sasikirono Rateh Lailatul Risdiyanti Serlia Nur Abifah Wiludjeng Widajati Wiludjeng Widayati Yenny WURYANDARI