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All Journal Agrovigor: Jurnal Agroekoteknologi Jurnal Hortikultura Agriprima : Journal of Applied Agricultural Sciences Jurnal Entomologi Indonesia JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) AGROLOGIA : Jurnal Ilmu Budidaya Tanaman Nusantara Science and Technology Proceedings Batara Wisnu : Indonesian Journal of Community Services Jurnal Riset Perkebunan (JRP)
Dita Megasari
Program Studi Agroteknologi, Fakultas Pertanian, Universitas Pembangunan Nasional Veteran Jawa Timur
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Education Engineering Environmental Science Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences

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Penekanan Penularan Bean Common Mosaic Virus oleh Efek Penghambat Makan Kitosan Terhadap Aphis craccivora Koch. (Bean Common Mosaic Virus Transmission Inhibition by Antifeedant Chitosan Against Aphis craccivora Koch.) Dita Megasari; Tri Asmira Damayanti; Sugeng Santoso
Jurnal Hortikultura Vol 29, No 2 (2019): Desember 2019
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v29n2.2019.p209-218

Abstract

Bean common mosaic virus (BCMV) merupakan virus penting pada kacang panjang di Indonesia. Salah satu cara untuk mengendalikan BCMV adalah dengan penggunaan kitosan. Pada penelitian sebelumnya, kitosan komersial dengan konsentrasi 0,9% dilaporkan mampu menekan infeksi BCMV yang ditularkan oleh Aphis craccivora dengan mekanisme yang belum diketahui. Penelitian ini bertujuan untuk mengkaji mekanisme kitosan dalam menekan transmisi BCMV yang ditularkan oleh A. craccivora. Kitosan yang diuji yaitu kitosan murni dengan konsentrasi 0,1%–1,1% dan kitosan komersial konsentrasi 0,9% sebagai pembanding. Kitosan diaplikasikan dengan cara penyemprotan daun dengan volume 3 ml/tanaman pada hari sebelum penularan BCMV menggunakan tiga ekor kutudaun yang mengandung virus. Peubah yang diamati, yaitu periode inkubasi, insidensi penyakit, keparahan penyakit, akumulasi virus pada tanaman dan kutudaun, serta deteksi gen CP BCMV, PR1, dan PR3 dengan RT-PCR. Secara umum perlakuan kitosan mampu menekan transmisi BCMV oleh A. craccivora. Periode inkubasi tanaman perlakuan berkisar antara 7–9 hari. Perlakuan kitosan murni menekan insidensi dan keparahan penyakit antara 40%–80% dan 35,71%–78,57% tergantung konsentrasi kitosan. Kitosan komersial menghambat insidensi dan keparahan penyakit sampai 100%. Perlakuan kitosan pada konsentrasi 0,9% baik kitosan murni maupun komersial menunjukkan akumulasi BCMV yang lebih rendah dibandingkan kontrol tanpa perlakuan, yaitu pada kitosan komersial 0,9% memiliki nilai absorbansi ELISA (NAE) sebesar 0,26 ± 0,29 dan pada kitosan murni 0,9% memiliki NAE sebesar 1,15 ± 1,69, sedangkan kontrol tanpa perlakuan memiliki NAE sebesar 3,13 ± 0,17. BCMV positif terdeteksi pada kutudaun menunjukkan bahwa kutudaun tidak makan inokulasi pada tanaman perlakuan. Amplifikasi gen CP BCMV dengan RT-PCR menunjukkan positif teramplifikasi pada semua perlakuan kitosan kecuali perlakuan kitosan komersial. Gen PR1 tidak teramplifikasi, sedangkan gen PR3 teramplifikasi pada semua perlakuan. Akumulasi PR3 tertinggi terdapat pada perlakuan 0,5% dan 0,7% dibandingkan dengan perlakuan lain dan kontrol. Berdasarkan hasil tersebut, perlakuan kitosan lebih berperan sebagai penghambat makan kutudaun daripada sebagai penginduksi ketahanan tanaman.KeywordsBCMV; Kutu daun; Pathogenesis-related protein; PCR; Vigna sinensis L.AbstractBean common mosaic virus (BCMV) is an important virus on beans in Indonesia. One of methods to control BCMV is by chitosan. The previous report showed that commercial chitosan was able to suppress BCMV infection transmitted by Aphis craccivora with an unknown mechanism. This study aimed to examine the mechanism of chitosan in suppressing the transmission of BCMV by A. craccivora. Chitosan used in this research is a pure chitosan with a concentration of 0.1%–1.1% and 0.9% of commercial chitosan as a comparison. Chitosan is applied by spraying the leaves at one day before the transmission of BCMV. BCMV transmitted by using three viruliferous aphids. The incubation period of virus, disease incidence, and severity, virus accumulation in plants and aphids by ELISA were observed, BCMV CP, PR1, and PR3 genes was detected by RT-PCR. In general, chitosan treatments suppressed the transmission of BCMV by A. craccivora. The incubation period ranges from 7–9 days. The pure chitosan treatments suppressed the disease incidence and severity ranged from 40%–80% and 35.71%–78.57%, respectively depends on concentration of chitosan. The commercial chitosan inhibited disease incidence and severity up to 100%. The treated plants at concentration 0.9% either pure or commercial chitosan showed accumulation of BCMV significantly lower in compared with untreated control plants, i.e. 0.9% in commercial chitosan had ELISA absorbance value (NAE) of 0.26 ± 0.29 and pure chitosan 0.9% had NAE of 1.15 ± 1.69 while the untreated control had an NAE of 3.13 ± 0.17. Further, BCMV were detected aphids which feed on treated plants during inoculation feeding period, indicating virus left over on aphids. Amplification of BCMV CP gene by RT-PCR showed positively amplified by all treatments except commercial chitosan treatment. PR1 gene are not amplified, whereas PR3 gene amplified in all the treatments, but the accumulation higher on treated plants at concentration 0.5% and 0.7% in compare with other treatments. Based on those results, chitosan treatment suppress the BCMV transmission by aphids due to the role of chitosan as anti-feedant which hampered aphids transmit the virus during inoculation feeding period rather than as resistance inducer.
Tingkat serangan ulat grayak tentara Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae) pada pertanaman jagung di Kabupaten Tuban, Jawa Timur, Indonesia Dita Megasari; Syaiful Khoiri
Agrovigor Vol 14, No 1 (2021): Maret
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v14i1.9492

Abstract

Spodoptera frugiperda atau ulat grayak tantara (fall army worm/FAW) adalah hama baru pada tanaman jagung di Indonesia. Laporan tentang serangan FAW masih terbatas. Penelitian ini bertujuan untuk mengetahui apakah S. frugiperda telah menyerang pertanaman jagung di Kabupaten Tuban dan menentukan tingkat serangannya. Sampling dilakukan dengan metode  Scouting  seperti yang direkomendasikan Balitsereal, Maros.  Penelitian dilaksanakan di 7 kecamatan di Kabupaten Tuban, Jawa Timur. Berdasarkan hasil penelitian menunjukkan bahwa seluruh lokasi pengamatan pada lahan jagung ditemukan serangan S. frugiperda. Presentase serangan hama baru S. frugiperda di Kabupaten Tuban berkisar antara 58% hingga 100%. Presentase serangan tertinggi (yakni 100%) terdapat pada lahan di Kecamatan Bancar, Kabupaten. Intensitas serangan S. frugiperda di bawah 60%. Intensitas serangan tertinggi yaitu di Kecamatan Bancar dengan nilai intensitas serangan 55%.
Biologi Spodoptera frugiperda JE Smith pada beberapa jenis pakan di laboratorium Dita Megasari; Ichsan Luqmana Indra Putra; Nanda Dwi Martina; Aulia Wulanda; Khusnul Khotimah
Agrovigor Vol 15, No 1 (2022): Maret
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v15i1.11978

Abstract

Spodoptera frugiperda merupakan hama baru pada tanaman jagung di Indonesia dan dilaporkan menyerang tanaman jagung di berbagai wilayah di Indonesia. Informasi tentang biologi hama S. frugiperda pada berbagai pakan perlu diketahui untuk menentukan stadia pengendalian paling efektif dan kesesuaian pakan bagi pertumbuhan dan perkembangannya. Penelitian ini bertujuan untuk mengetahui lama siklus hidup dan periode perkembangan S. frugiperda yang diberi pakan berbeda. Penelitian ini menggunakan tujuh pakan daun yang berbeda, yaitu: kontrol (jagung), selada, pakcoy, kangkung, bawang daun, bayam cabut hijau, dan bayam duri hijau. Penelitian dilakukan dengan memelihara 10 ekor tiap unit percobaan. Perkembangan stadia perkembangan hama dicatat, dan pupa diukur panjang serta bobotnya. Hasil penelitian menunjukkan, inang yang berbeda dapat mempengaruhi pertumbuhan dan perkembangan S. frugiperda. Perlakuan yang menunjukkan siklus hidup paling pendek adalah pakcoy. 
Pengendalian Aphis craccivora Koch. dengan kitosan dan pengaruhnya terhadap penularan Bean common mosaic virus strain Black eye cowpea (BCMV-BlC) pada kacang panjang Dita Megasari; Tri Asmira Damayanti; Sugeng Santoso
Jurnal Entomologi Indonesia Vol 11 No 2 (2014): September
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (829.2 KB) | DOI: 10.5994/jei.11.2.72

Abstract

Aphis craccivora is one of the important pests on yard long bean. It causes direct damage and also has an indirect effect as insect vector of the Bean common mosaic virus (BCMV). The research was done to test the effectiveness of chitosan in suppressing aphid population growth, feeding preference and its ability in transmiting BCMV. Chitosan with concentration ranging of 0.1–1.0% were applied on leaves using spraying method at 1 day before BCMV transmission. BCMV was transmitted by using 3 individuals of viruliferous aphids on each plant. Results show that chitosan treatments on leaves or plants significantly suppressed the population and feeding preferences of A. craccivora. Further, treated plants showed lower disease incidence, severity and BCMV titre significantly compared with untreated control plants. The positive effects of chitosan in suppressing population growth as feeding preferences and BCMV transmission might be due to the anti-feedant effect of chitosan on A. craccivora. Based on the result, chitosan at concentration 0.9% is the most effective concentration in suppressing BCMV and its vector A. craccivora.
Pelatihan dan Pendampingan Teknologi Asap Cair Menggunakan Limbah Pertanian di Kecamatan Parengan, Tuban Adi Rastono; Refa Firgiyanto; Pitri Ratna Asih; Ega Faustina; Dita Megasari
JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) VOL. 2 NOMOR 2 SEPTEMBER 2018 JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.422 KB) | DOI: 10.30595/jppm.v2i2.2179

Abstract

ABSTRAKPada era globalisasi persoalan mengenai pencemaran dan kerusakan lingkungan menjadi suatu permasalahan yang masih belum bisa dipecahkan oleh masyarakat secara berkelanjutan. Salah satu penyebabnya adalah penumpukan limbah seperti limbah pertanian. Tujuan kegiatan pengabdian ini adalah untuk mengolah limbah pertanian menjadi produk asap cair yang multifungsi dengan menggunakan metode pirolisis.Kegiatan pengabdian dilaksanakan mulai bulan Agustus sampai dengan Desember 2017 di Desa Sukorejo Kecamatan Parengan Kabupaten Tuban dengan kelompok sasaran adalah petani. Metode kegiatan ini meliputi a) penyuluhan dengan tujuan meningkatkan pemahaman petani dalam mengelola limbah pertanian menjadi asap cair, b) Difusi Iptek melalui pembuatan peralatan asap cair dengan menggunakan metode pirolisis, c) Pelatihan dan pendampingan dalam proses pembuatan asap cair berbahan limbah pertanian disertai dengan monitoring dan evaluasi. Hasil dari kegiatan adalah meningkatkan pengetahuan dan ketrampilan mitra, terciptanya instalansi alat pembuatan asap cair dan asap cair berbahan limbah pertanian yang multifungsi.  Kata Kunci: Asap cair, Limbah, Pelatihan, Pendampingan, Pestisida.
The Effect of Temperature Pressure on Multiantagonists Streptomyces sp., Tricho-derma sp. Biological Control of Fusarium oxysporum Wilt Pathogens Penta Suryaminarsih; Dita Megasari; Tri Mujoko
Nusantara Science and Technology Proceedings Seminar Nasional Magister Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2020.0609

Abstract

Climate change due to global warming can increase soil temperatures. The increase in soil temperature can be influenced by air temperature which can affect the life of soil microorganisms. Streptomyces sp. and Trichoderma sp. is a soil microorganism that acts as a Biological Control Agent (APH) of plant pests and diseases. This study aims to determine the inhibitory ability of Streptomyces sp. and Trichoderma sp. at high temperature against Fusarium sp. causes of wilt disease in chilies. This research used factorial completely randomized design. The first factor is room temperature 29-31 ° C, temperature 50 ° C, and temperature 70 ° C. The second factor is the type of andosol soil (UPN land) and regusol (Pare village land). Observations were made on the number of colonies of Streptomyces sp. and Trichoderma sp. as well as inhibition against the fungus Fusarium sp. The results showed that the multiantagonist inhibition of Streptomyces sp. and Trichoderma sp. against F. ox-ysporum at 70 ° C in 90% andosol soil and the lowest at 70 ° C on 31% regusol soil.
Prospects of Biological Control of Green Ladybugs (Nezara viridula) Using Streptomyces spp. Laksamana Agadhia Raharjo; Penta Suryaminarsih; Dita Megasari
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1504

Abstract

Ecosystem damage due to the use of chemical pesticides increases every year, therefore alternative searches need to be known and developed. APH (Biological Control Agency) is a beneficial microbe that can control pest and disease populations. Streptomyces spp. Is a gram-positive soil bacterium that can be used against green ladybugs because Streptomyces spp. Able to produce chitinase enzymes that can break down chitin compounds in Exo – Skeleton Arthropod animals The purpose of this study was to determine the prospects for the use of Streptomyces spp. as APH of green shieldbugs.The description of this article includes: research results of Streptomyces spp., morphology, bio-ecology and bio-molecular green shieldbugs (Nezara viridula), as well as prospects for the use of Streptomyces spp. the results show that the use of Streptomyces spp. is able to control green ladybugs, because the chitinase enzyme produced can break down the chitin compound.The development of Streptomyces spp. has a lot of space, developments in the production of chitinase enzymes can be implemented and have clear potential.
The Incidence and Severity of Downy Mildew Disease on Local Madurese Maize Crops in Sumenep district, East Java, Indonesia Syaiful Khoiri; Abdiatun Abdiatun; Khairatul Muhlisa; Achmad Amzeri; Dita Megasari
Agrologia Vol 10, No 1 (2021): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajibt.v10i1.1295

Abstract

In Madura island, corn is the main commodity that is widely planted with an area of 301,725 ha or about 30% of the area of maize in East Java. Madura Island has local cultivars, such as: Tambin, Talango, Guluk-guluk, Manding, and Kretek. Efforts to increase production are continuously being made, starting from improving varieties until managing plant pests. One of the main diseases in maize is downy mildew. However, information about the incidence, incidence, severity, and species that cause downy mildew in local cultivars has not been reported. So, this study aims to identify the causes of downy mildew in local cultivars of Madura and disease severity in the field. The research method is a survey on local maize centers. Sampling was done by using the diagonal sampling method. Each plant sample was observed for symptoms of disease and scoring to calculate the value of disease severity. Fungi identification was carried out by microscopic observation of the fungus. The results showed that the cause of downy mildew in Madura local maize in Sumenep Regency was P. maydis. The highest incidence, disease severity, and AUDPC value after 4 MST were found in Guluk-guluk cultivars in Padangdangan Village, but had the lowest disease progression rate values. Meanwhile, the highest rate of disease progression was found in the Manding cultivar in Mandala Village. Based on the resistance category, Talango cultivar had the best resistance when compared to other cultiva.Keywords: AUDPC, downy mildew, disease progress, Madurese maize, Peronosclerospora maydis
Pengaruh Biopestisida Fobio dan Agens Hayati Trichoderma sp., terhadap Penyakit Layu Fusarium pada Bawang Merah Farisa; Dita Megasari; Sri Wiyatiningsih
Agriprima : Journal of Applied Agricultural Sciences Vol 7 No 1 (2023): MARCH
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v7i1.522

Abstract

Pengendalian penyakit tanaman bawang merah hingga saat ini masih mengandalkan fungisida kimia yang dapat mencemari lingkungan, sehingga perlu dilakukan pengendalian secara hayati dan ramah lingkungan. Alternatif yang dapat diterapkan adalah menggunakan Biopestisida Fobio dan agens hayati Trichoderma sp. Tujuan penelitian ini adalah untuk mengetahui respon aplikasi Biopestisida Fobio dan agens hayati Trichoderma sp. terhadap penyakit layu fusarium pada bawang merah. Penelitian dilaksanakan pada bulan Agustus-Oktober 2022 di Kecamatan Kedopok, Kota Probolinggo. Penelitian menggunakan Rancangan Petak Terbagi dengan dua faktor, yaitu konsentrasi Biopestisida Fobio terdiri dari 3 taraf yaitu petak utama adalah F0 (kontrol atau perlakuan pestisida kimia), F1 (Fobio 5 ml/liter), F2 (Fobio 7,5 ml/liter) dan anak petak yaitu konsentrasi Trichoderma sp. terdiri dari 3 taraf yaitu T0 (kontrol atau perlakuan pestisida kimia), T1 (Trichoderma sp. 10 ml/liter), T2 (Trichoderma sp. 20 ml/liter) sehingga terdapat 9 kombinasi perlakuan. Parameter pengamatan meliputi periode inkubasi, kejadian penyakit, berat basah umbi, dan berat kering umbi. Hasil penelitian dari semua kombinasi perlakuan tidak berbeda nyata. Hasil perlakuan aplikasi Biopestisida Fobio menunjukkan bahwa tanaman bawang merah berbeda nyata terhadap periode inkubasi pada perlakuan F2 dengan nilai rata-rata tertinggi yaitu 20 hst. Kejadian penyakit terendah terdapat pada perlakuan F2 pada umur 28 dan 42 hst. Perolehan berat basah paling tinggi diperoleh pada perlakuan F2T0 yaitu 2,128 kg. Perolehan berat kering tertinggi yaitu pada perlakuan F0T2.
REFUGIA PLANTS AS NATURAL ENEMY MICROHABITAT FOR PEST CONTROL ON MANGO (Mangifera indica L.) FARMER GROUP SUKODADI PASURUAN Wiwin Windriyanti; Noni Rahmadhini; Dita Megasari; Ramadhani Mahendra Kusuma; Yudi Nur Supriadi
Batara Wisnu : Indonesian Journal of Community Services Vol. 3 No. 2 (2023): Batara Wisnu | Mei - Agustus 2023
Publisher : Gapenas Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53363/bw.v3i2.187

Abstract

The utilization of refuge plants as microhabitats for natural enemies has been explored as a sustainable approach to pest control in mango cultivation. This community service project aimed to assess the feasibility and effectiveness of implementing refuge plants for pest management in the Sukodadi Farmer Group, Pasuruan Regency. The background highlighted the challenges faced by mango farmers, including pest infestations and the negative impacts of chemical pesticides on the environment and human health. The methodology involved socialization and demonstrating the use of refugees around mango fields. The participation and enthusiasm of the farmers were remarkable, as they gained valuable knowledge and confidence in implementing environmentally friendly pest and disease management practices. The farmers observed a reduction in pest populations and improved crop health. The active involvement of the farmers in the project increased their understanding of sustainable pest management practices and enhanced their confidence in implementing these methods. This community service project contributes to the knowledge and implementation of integrated pest management strategies in mango cultivation. The findings emphasize the significance of utilizing refuge plants as microhabitats for natural enemies to enhance pest control and promote the sustainability of mango production. The outcomes serve as a valuable resource for farmers, extension workers, and policymakers involved in agricultural practices in Sukodadi and similar regions
Co-Authors Abdiatun Abdiatun Achmad Amzeri Adi Rastono Aulia Wulanda Dheananda Fyora Hermansyah Azari Ega Faustina Farisa Indra Putra, Ichsan Luqmana Khairatul Muhlisa Khusnul Khotimah Laksamana Agadhia Raharjo Nanda Dwi Martina Noni Rahmadhini Penta Suryaminarsih Penta Suryaminarsih Pitri Ratna Asih Ramadhani Mahendra Kusuma Refa Firgiyanto Shafira Desty Adisa Sri Wiyatiningsih Sugeng Santoso Sugeng Santoso Syaiful Khoiri TRI ASMIRA DAMAYANTI Tri MUJOKO Windriyanti, Wiwin YUDI NUR SUPRIADI