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All Journal Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Eddyman W Ferial
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Deteksi gen pneumolysin (ply) Streptococcus pneumoniae pada Sampel Klinis Usia Lanjut secara Kultur dan PCR Muthmainnah Muthmainnah; Mochammad Hatta; Muh. Nasrum Massi; Firdaus Hamid; Irawati Djaharuddin; Eddyman W Ferial; Andi Alfian Zainuddin; Mustika Hutabarat Sari
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 12 No 1 (2020): Jurnal Riset Kesehatan Poltekkes DepKes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.872 KB) | DOI: 10.34011/juriskesbdg.v12i1.850

Abstract

ABSTRACT Streptococcus pneumoniae, a gram-positive bacterium of normal flora but can be a pathogenic bacterium that causes pneumonia if the immune system declines. Community pneumonia ranks fourth and top ten diseases treated per year. Populations that are susceptible to pneumonia are children aged less than 2 years, old age. The Purpose aim to detect ply genes in sputum with suspected pneumonia in elderly as a virulence factor of S. pneumoniae by culture and PCR. This research method is a descriptive study to detect the virulence factor of the S. pneumoniae ply gene in the sputum of patients with suspected pneumonia in the elderly with the culture and PCR methods. Conventionally (culture method) obtained 1 sample from 56 positive samples of S. pneumoniae gram positive bacteria on the molecular method (PCR) there were 7 positive samples of S. pneumoniae bacteria from 56 samples. In detecting ply genes in sputum with suspected pneumonia in elderly as a virulence factor from S. pneumoniae bacteria were culturally detected 1 positive sample from 56 samples and by PCR method detected 7 positive samples from 56 samples. In detecting ply genes using culture and PCR it can be said that the PCR method is more accurate, fast and precise in detecting the S. pneumonia gene in sputum samples with suspected pneumonia in elderly.
Co-Authors Andi Alfian Zainuddin Firdaus Hamid Irawati Djaharuddin Mochammad Hatta Muh. Nasrum Massi Mustika Hutabarat Sari Solly Aryza