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All Journal Jurnal Ilmu Ternak dan Veteriner Animal Production : Indonesian Journal of Animal Production
Eros Sukmawati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Freezing capacity of sperm on various type of superior bulls Sukmawati, Eros; Arifiantini, R. I.; Purwantara, B.
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 3 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v19i3.1079

Abstract

Low quality of sperm after freezing and thawing process due to changes in extreme temperature and osmolarity. The sperm freezing capability and sperm membrane damage was to evaluate by measuring the levels of malondialdehyde (MDA) of  Simmental, Limousin and Friesian Holstein (FH) bull of a total of 10 bulls aged 4-8 years. Data were analyzed with a linear model (GLM) and Duncan s test. Results showed that breed influence sperm motility and MDA levels but not in the membrane integrity (MI) and viability. The FH bull had a low of recovery rate (RR) 57.53± 1.74% with high MDA level (0.81± 0.31 nmol/108 sperm level) and Limousine had the highest RR (59.70 ± 3.23% ) with the low MDA (0.52±0.25 nmol/108 sperm). Freezing decreased the sperm motility, viability and MI of all bulls. Sperm motility, viability and MI decreased by 28.32±1,45% and 29.73±1.54%, 21.58±4.09% and 22.55± 5.60% and 21.25±6.86% and 23.51±6.05 % respectively. Key Words: Freezing, Bulls, Freezing Capability, Malondialdehyde
The Influence of Breed and Type of Extender on the Quality of Bull Semen Sukirman, Iman; Sukmawati, Eros; Rasad, Siti Darojah; Solihati, Nurcholidah
ANIMAL PRODUCTION Vol 21, No 2 (2019)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (47.07 KB) | DOI: 10.20884/1.jap.2019.21.2.641

Abstract

This study aimed to determine the influence of breed and type of extenders on frozen semen quality of cows at BIB Lembang. The experimental study was conducted in a Factorial Randomized Block Design (RBD) with two factors. The first factor was four cow breeds, i.e. Ongole Cross (PO), Brahman (BR), Simmental (SM) and Limousin (LM), and the second factor was two types of extender, i.e. Skim-Egg Yolk (SKT) and AndroMed® (AND), all repeated four times. The observed variables were percentage of spermatozoa motility and intact plasma membrane (IPM). All data obtained were analyzed using a general linear model (IBM SPSS ver. 23). The results demonstrated an interaction between breed and the type of diluent to motility. Breeds showed significantly different motility but non-significantly different intact plasma membrane (MPU) of semen. The type of diluent did not significantly affect motility and intact plasma membrane (MPU) of the frozen semen. The effect of the breed on BR motility was lower and significantly different from PO, LM and SM. The types of diluent did not significantly affect motility, MPU. The results showed that SKT was lower than AND, it was indicative effect of breed on intact plasma membrane (MPU) PO was lower than BR, LM and SM and the effect of the type of diluent on whole plasma membrane (MPU) AND is lower than SKT. It can be concluded that breed influences the motility of semen. The lowest motility reduction in frozen semen is Brahman cattle by using skim-egg yolk extender.
Freezing capacity of sperm on various type of superior bulls Eros Sukmawati; R. I. Arifiantini; B. Purwantara
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 3 (2014): SEPTEMBER 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v19i3.1079

Abstract

Low quality of sperm after freezing and thawing process due to changes in extreme temperature and osmolarity. The sperm freezing capability and sperm membrane damage was to evaluate by measuring the levels of malondialdehyde (MDA) of  Simmental, Limousin and Friesian Holstein (FH) bull of a total of 10 bulls aged 4-8 years. Data were analyzed with a linear model (GLM) and Duncan 's test. Results showed that breed influence sperm motility and MDA levels but not in the membrane integrity (MI) and viability. The FH bull had a low of recovery rate (RR) 57.53± 1.74% with high MDA level (0.81± 0.31 nmol/108 sperm level) and Limousine had the highest RR (59.70 ± 3.23% ) with the low MDA (0.52±0.25 nmol/108 sperm). Freezing decreased the sperm motility, viability and MI of all bulls. Sperm motility, viability and MI decreased by 28.32±1,45% and 29.73±1.54%, 21.58±4.09% and 22.55± 5.60% and 21.25±6.86% and 23.51±6.05 % respectively.
Co-Authors B. Purwantara B. Purwantara Nurcholidah Solihati R. I. Arifiantini Rasad, Siti Darojah Sukirman, Iman