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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kimia Terapan Indonesia JURNAL SELULOSA
Muhammad Hanafi
Research Center for Chemistry, Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Materials Science & Nanotechnology

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Synthesis and cytotoxicity assay using Brine Shrimp Lethality Test of Cinchonidine Isobutyrate Ester Mario Mario; Puspa Dewi Lotulung; Gian Primahana; Sylvia Rizky Prima; Muhammad Hanafi
Jurnal Kimia Terapan Indonesia Vol 19, No 1 (2017)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.382 KB) | DOI: 10.14203/jkti.v19i1.328

Abstract

This research was aimed to synthesize cinchonidine isobutyrate ester and conduct a preliminary assay for anticancer agent using cytotoxicity assay to Artemia salina Leach larva, or also known as brine shrimp lethality test (BSLT). Cinchonidine, a compound that has quinoline rings and quiniclidine ring, is a quinine analogue and stereoisomer of cinchonine. Cinchonidine is predicted to have anticancer activity. Synthesized ester was aimed to gain higher lipophilicity. Higher lipophilicity makes it easier for the compund to pass through cell membrane. The esterification process used DMAP as a catalyst, DCC as an activator, and isobutyric acid as a carboxilyc acid. Isobutyric acid is a type of short chained fatty acid that usually acts as an anticancer prodrugs. The product is identified by ESI-MS, FT-IR, 1H-NMR, dan 13C-NMR. Ester cinchonidine isobutyrate is gelatinous and colourless with yield of 21,77%. BSLT result showed that cinchonidine isobutyrate ester had LC50 value of 75.16 ppm which was more toxic than cinchonidine that had LC50 value of 99.2 ppm. It was proved that higher lipophilicity could increase pharmacology activity
Produksi β-Glukosidase Aspergillus niger BIO 2173 dengan Fermentasi Padat Menggunakan Substrat Dedak Sri Sugiwati; Maggy Thenawidjaja Suhartono; Muhammad Hanafi; Hanifah Nuryani Lioe
JURNAL SELULOSA Vol 8, No 01 (2018): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.498 KB) | DOI: 10.25269/jsel.v1i01.221

Abstract

Production of β-Glucosidase Aspergillus niger BIO 2173 on Solid State Fermentation Using Rice Bran as SubstrateAbstractβ-Glucosidase (EC 3.2.1.21) is a part of the cellulase enzyme complex which acts synergistically with exoglucanase and endoglucanase to hydrolyze cellulose into glucose. The purpose of this study was to obtain the maximum fermentation conditions for production of b-glucosidase Aspergillus niger BIO 2173 with solid state fermentation using rice bran as fermentation substrate. The factors that affect the production of b-glucosidase which consist of initial pH of the fermentation medium, incubation period, ratio of water content to fermentation substrate, incubation temperature and addition of the Mandel’s mineral salts solution were examined in the study. The results showed that maximum fermentation conditions for β-glucosidase production were at initial of fermentation pH of 2,0, incubation period of 7 days, ratio of water content to substrate of 1:1, and incubation temperature of 32oC. Addition of Mandel’s mineral salts solution to the fermentation substrate at maximum fermentation conditions increased the activity and specific activity of β-glucosidase crude extract up to 5,24 ± 0,57 U/mL and 2,46 ± 0,04 U/mg, respectively.Abstrakβ-Glukosidase (EC 3.2.1.21) merupakan bagian dari enzim multi kompleks selulase, yang bekerja secara sinergis dengan eksoglukanase dan endoglukanase menghidrolisis selulosa menjadi glukosa. Tujuan dari penelitian ini adalah mendapatkan kondisi fermentasi maksimum untuk produksi β-glukosidaseAspergillus niger BIO 2173 dengan fermentasi media padat menggunakan substrat dedak. Pengujian dilakukan terhadap faktor-faktor yang mempengaruhi produksi b-glukosidase, yaitu pH awal medium fermentasi, waktu inkubasi, perbandingan kandungan air terhadap substrat medium fermentasi, suhu inkubasi dan penambahan larutan garam mineral Mandels. Hasil penelitian menunjukkan bahwa kondisi fermentasi maksimum untuk produksi b-glukosidase adalah pada pH awal medium fermentasi 2,0; waktu inkubasi 7 hari, perbandingan kandungan air terhadap substrat medium fermentasi 1:1, dan suhu inkubasi 32oC. Penambahan larutan garam mineral Mandels ke dalam substrat fermentasi pada kondisi fermentasi maksimum menyebabkan peningkatan aktivitas dan aktivitas spesifk ekstrak kasar b-glukosidase masing-masing sebesar 5,24 ± 0,57 U/mL dan 2,46 ± 0,04 U/mg protein. Kata kunci: β-glukosidase, Aspergillus niger, dedak padi, fermentasi padat, ekstrak kasar
The Ethyl Acetate Fraction of Torbangun (Coleus amboinicus L.) Leaves Increasing Milk Production With Up-Regulated Genes Expression of Prolactin Receptor Ade Chandra Iwansyah; Rizal Martua Damanik; Lilik Kustiyah; Muhammad Hanafi
Journal of Tropical Life Science Vol. 9 No. 2 (2019)
Publisher : Journal of Tropical Life Science

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Abstract

This study aim was to determine lactagogue effect of torbangun leaves to plasma levels of lactogenic hormone and gene expression of their receptors in mammary glands of lactation rats. Lactagogue activity was evaluated by volume of milk was produced by the rats treated with commercial milk booster contained ‘katuk' leaves extract (AF), ethyl acetate fraction of torbangun leaves (EA), water extraction of torbangun leaves (AQ) and kaempferol (KP). Lactating rats (n=5) of Sprague dawley with six pups were fed with AF, EA, AQ, and KP in the amount of 50 mg/kg, 30 mg/kg, 80 mg/kg and 60 mg/kg body weight, respectively. The feed was given orally every two days and starting from day 2 after giving birth until day 28. The volume of milk was estimated by the increment pup weight after breastfed. The levels of serum lactogenic hormones were determined by ELISA methods. Moreover, in order to measure the gene expression of the lactogenic hormone's receptors in the mammary gland a real time - reverse transcription polymerase chain reaction (RT-PCR) method was performed. The results showed that ethyl acetate fraction of torbangun leaves (EA) (a) was not significantly stimulating the synthesis of serum prolactin and estradiol at day 14 and day 28 lactation period, (b) down-regulated the gene expression of estradiol receptor (ERα) at day 28, and (c) up-regulated the gene expression of prolactin receptor (PRLR) in mammary gland at day 14 and day 28. This study indicated that ethyl acetate fraction of torbangun leaves was induced milk production, within up-regulated the gene expression of prolactin receptor (PRLR) in the mammary gland of lactation rats.
Co-Authors Ade Chandra Iwansyah Gian Primahana Hanifah Nuryani Lioe Lilik Kustiyah M. Rizal Martua Damanik Maggy Thenawidjaja Suhartono Mario Mario Puspa Dewi Lotulung Sri Sugiwati Sylvia Rizky Prima