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Risanti Situmorang
Universitas Sumatera Utara, Medan, Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science

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Phytochemical screening of bunga rosella (Hibiscus sabdariffa L) and antimicrobial activity test Hermawan Purba; Helen Anjelina Simanjuntak; Risanti Situmorang
Jurnal Pendidikan Kimia (JPKim) Vol 12, No 2 (2020): August
Publisher : Pascasarjana Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (289.42 KB) | DOI: 10.24114/jpkim.v12i2.19398

Abstract

The purpose of this study was to determine the antimicrobial activity of rosella infusa (Hibiscus sabdariffa L.). Antimicrobial inhibition test using infusa of rosella (Hibiscus sabdariffa L.) against Staphylococcus aureus and Escherichia coli bacteria at a concentration of infusa 100%; 75%; and 50%. The concentration has highest potential antibacterial of infusa for Staphylococcus aureus is 100% witch has diameter of 13.6 mm and for Escherichia coli bacteria is 100%, its show that diameter of zone inhibitory zone is 13.3 mm. The Results of phytochemical screening show that infusa of rosella (Hibiscus sabdariffa L.) contains flavonoids, saponins, and tannins. Based on the data above show that the infusa of rosella flower has high antibacterial potential. Keywords: Hibiscus sabdariffa L., antimicrobial activity, Staphylococcus aureus, Escherichia coli
Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method Kasta Gurning; Sifikal Lumbangaol; Risanti F. R Situmorang; Saronom Silaban
Jurnal Pendidikan Kimia (JPKim) Vol 13, No 2 (2021): August
Publisher : Pascasarjana Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.348 KB) | DOI: 10.24114/jpkim.v13i2.26984

Abstract

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH
Co-Authors Helen Anjelina Simanjuntak Hermawan Purba Kasta Gurning Saronom Silaban Sifikal Lumbangaol