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E. Suryanto
Faculty of Animal Science, Gadjah Mada University, Jl. Fauna No.3, Bulaksumur, Yogyakarta 55281
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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COMPARATIVE STUDY ON ANGIOTENSIN CONVERTING ENZYME INHIBITORY ACTIVITY OF HYDROLYSATE OF MEAT PROTEIN OF INDONESIAN LOCAL LIVESTOCKS Jamhari, J.; Yusiati, L.M.; Suryanto, E.; Cahyanto, M.N.; Erwanto, Y.; Muguruma, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 1 (2013): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.1.27-33

Abstract

The experiment was conducted to investigate the angiotensin converting enzyme (ACE) inhibitoryactivity of hydrolysate in meat protein of Bali cattle, Kacang goat, native chicken, and local duck. Themeats of Bali cattle, Kacang goat, native chicken, and local duck were used in this study. The meatswere ground using food processor added with aquadest to obtain meat extract. The meat extracts werethen hydrolyzed using protease enzymes to obtain hydrolysate of meat protein. Protein concentration ofmeat extract and hydrolysate of meat protein were determined, and were confirmed by sodium dodecylsulfate - poly acrylamide gel electrophoresis (SDS-PAGE). ACE inhibitory activity of hydrolysate ofmeat protein derived from Bali cattle, Kacang goat, native chicken, and local duck was also determined.The results showed that protein concentration of hydrolysate of meat protein of Bali cattle, Kacang goat,native chicken, and local duck meat was significantly higher than their meat extracts. SDS-PAGEanalysis indicated that hydrolysate of meat protein of Bali cattle, Kacang goat, native chicken, and localduck had more peptides with lower molecular weight, compared to their meat extracts. Hydrolysate ofmeat protein of Bali cattle, Kacang goat, native chicken, and local duck had potencies in inhibiting ACEactivity, so it will potentially reduce blood pressure.
THE EFFECT OF METHANOGENIC INHIBITOR FEED ON PROPIONIC ACID AND LAMB MEAT CHEMICAL QUALITY Sondakh, E.H.B.; Yusiati, I.M.; Hartadi, H.; Suryanto, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 3 (2012): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.3.183-188

Abstract

This study aimed to determine the effect of medium chain fatty acids (MCFA) on propionic acidsand lamb meat chemical quality. The treatment given was R1: feed without medium chain fatty acids(MCFA), while R2 dan R3 were the feed contained 1.0% and 1.5% of MCFA, respectively. The twelveheads of lambs yearling weight of 16-17 kg were used as materials. Biological trial was done for threemonths and then was slaughtered. Before being slaughtered, the animal was taken rumen fluid to beanalyzed for propionic acid. The carcass was sampled to be analyzed for chemical composition,cholesterol and fatty acids content. This study showed that methanogenic inhibitor feed with 1.0-1.5%MCFA could be used as sheep feed, and the results: the propionic acid content in rumen increased 29.59– 36.11%. The cholesterol content decreased 7.14-10.06%. For the meat fatty acids composition,unsaturated fatty acids increased 9.05 – 17.96%. while saturated fatty acid decreased 6.59 – 11.88%.
CARCASS QUALITY, MARBLING AND CHOLESTEROL CONTENT OF MALE BALI CATTLE FED FERMENTED COCOA SHELL Suryanto, E.; Bulkaini, B.; Ashari, A.; Karda, I . W.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.249-255

Abstract

The experiment was conducted to evaluate the effect of fermented cacao shell on the carcassquality, percentage of non carcass components, marbling and cholesterol content of Bali cattle. NineBali cattle of 1.5-2 year old and 165-175 kg weight were grouped into three feeding trials. The firstgroup P0 was fed ration containing 30% cacao shell fermented with its own microorganism and 70%corn straw, the second group P1 was fed ration containing 30% cacao shell fermented with ruminal fluidand 70% corn straw, and the third group P2 was fed ration containing 30% cacao shell fermented withBioplus and 70% corn straw. All cattle were also given commercial concentrate amounting to 1% oftheir bodyweight. The cattle were slaughtered at the end of feeding trial and their carcasses, meat andnon carcass components were analysed. Experimental design used was completely randomized design.The results showed that carcass percentage, back fat thickness, rib eye area and meat index of Bali cattle were as follows group PO 53.33%, 3.08 mm, 59.65 cm2, 0.79%; group P1 52.64%, 5.31 mm, 58.52 cm,0.82% and group P2 5.32%, 5.7 mm, 57.75 cm, 0.79%, respectively. The marbling and cholesterolcontent of Bali beef were group PO 2.65% and 71.25 mg/100g, group P1 2.12% and 48.75 mg/100g,and P2 2.63% and 74.50 mg/100g. It could be concluded that fermented cacao shell could be used asfeed ingredient without any effect on the carcass and meat quality, marbling and cholesterol contents ofmale Bali beef.
PHYSICAL, CHEMICAL AND SENSORY PROPERTIES OF KENARI (Canariun indicum L.) SHELL LIQUID SMOKE-IMMERSED-BEEF ON DIFFERENT LEVEL OF DILUTION Yusnaini, Y.; Soeparno, S.; Suryanto, E.; Armunanto, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 1 (2012): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.1.27-33

Abstract

This research was aimed to study the effect of the dilution level of kenari shell liquid smoke to thephysical, chemical and sensory properties of beef. Longissimus dorsi of beef immersed on Kenari shellliquid smoke on the level of dilution 1x, 2x, 3x, 4x, and 5x during 15 minutes with five replicatesfollowing Randomized Complete Design. The results showed that the higher level of dilution of liquidsmoke kenari shell caused the physical, chemical and sensory properties which get closer to the qualityof the control samples. The level of dilution affected almost all the properties, except the water andprotein content. It was concluded that the lower levels of dilution caused the more affected into thephysical, chemical and sensory properties of beef, except the water and protein content.
PURIFICATION OF ANGIOTENSIN CONVERTING ENZYME INHIBITORY PEPTIDE DERIVED FROM KACANG GOAT MEAT PROTEIN HYDROLYSATE Jamhari, J.; Yusiati, L.M.; Suryanto, E.; Cahyanto, M.N.; Erwanto, Y.; Muguruma, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 4 (2013): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.4.239-246

Abstract

The objective of this study was to identify the Angiotensin Converting Enzyme (ACE) inhibitorypeptide derived from Kacang goat meat protein hydrolysate. Kacang goat meat loin section washydrolyzed with pepsin, trypsin and chymotrypsin. Protein hydrolysate of Kacang goat meat was thentested the protein concentration and ACE inhibitory activity. ACE inhibitory peptide of the proteinhydrolysate was purified through several steps of purification by column SEP-PAK Plus C18 Cartridgeand RP-HPLC using a Cosmosil column 5PE-SM, 4.6 x 250　mm. The sequence of amino acid of ACEinhibitory peptide was identified by amino acid sequencer. The results showed that amino acidssequence of ACE inhibitory peptide derived from protein hydrolysate of Kacang goat meat was leu-thrglu-ala-pro-leu-asn-pro-lys-ala-arg- asn-glu-lys. It had a molecular weight (MW) of 1581 and occurredat the position of 20th to 33rd residues of b-actin of goat meat protein (Capra hircus). The ACE inhibitoryactivity (IC50) of the peptide was 190 mg/mL or 120 mM.
THE EFFECT OF TYPE OF FEED AND SLAUGHTER AGE ON THE PERFORMANCES AND CARCASS CHARACTERISTIC OF MALE ARAB CHICKEN Suryanto, E.; Sasongko, H.; Maryam, M.; Santosa, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 34, No 3 (2009): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.34.3.181-188

Abstract

An experiment was conducted to evaluate the effect of type of feed and slaughter age on the performancesand carcass characteristics of male Arab chicken. Thirty six male Arab chicken of seven weeks old weredivided into two groups, each group consisted of three cages with six chicken in each group. Two types ofration consisted of Ration One (Par-S + corn) containing 2904 kcal ME and 17 % CP, and Ration Two (BRII) containing 3100 kcal ME and 19% CP. The chicken were raised for seven weeks, weekly weighed, andrandomly taken for slaughter at 12 and 14 week old. The data collected were performances and carcasscharacteristics of the chicken. The data were analyzed using analysis of variance of completely randomiseddesign. The results showed that the chicken fed BR II consumed more protein and energy (483.7 g CP and7868.8 kcal ME) compared to the chicken fed BR I (444.0 g CP and 7586.1 kcal ME). Besides, the chickenfed BR II produced heavier final body weight (1142.5 g) than the chicken fed with BR I (1048.6 g) (P <0,05). Type of ration affected the tenderness of chicken. Slaughter age influenced carcass weight andcarcass physical composition of the chicken (P<0.05). Slaughter age affected the physical characteristic ofmeat. It could be concluded that male Arab chicken fed ration containing higher CP and ME (higher feeddensity) consumed more protein and energy, and finally had heavier final body weight. Slaughter age influencedcarcass weight, carcass physical composition and meat characteristics of the male Arab chicken.
Co-Authors A. Ashari, A. Bulkaini (Bulkaini) E.H.B. Sondakh H. Hartadi H. Sasongko I . W. Karda, I . W. I.M. Yusiati J. Jamhari L.M. Yusiati M. Maryam M. Muguruma M.N. Cahyanto R. Armunanto R. Santosa S. Soeparno Y. Erwanto Y. Yusnaini