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Rebecca Christiana
Magister Biology, Satya Wacana Christian University, Jl. Diponegoro 52-60, Salatiga 50711
Chemical Engineering, Chemistry & Bioengineering Chemistry

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PHOTODEGRADATION AND ANTIOXIDANT ACTIVITY OF CHLOROPHYLL a FROM SPIRULINA (Spirulina sp.) POWDER Rebecca Christiana; Hari Kristopo; Leenawaty Limantara
Indonesian Journal of Chemistry Vol 8, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.791 KB) | DOI: 10.22146/ijc.21628

Abstract

Research on chlorophyll a from Spirulina (Spirulina sp.) powder has been done in order to determine chlorophyll a content, to compare degradation pattern and kinetics degradation of chlorophyll a and crude extract as well as to investigate the difference of antioxidant activity of chlorophyll a with or without irradiation. Chlorophyll a content in spirulina powder was calculated by using Porra's equation. Irradiation and recovery ability of chlorophyll a were measured by using volpi light (Intralux 6100) equipped with daylight filter. The antioxidant activity of chlorophyll a was determined by using DPPH method. The results showed that chlorophyll a content was 2.886 μg/g dry weight. Chlorophyll a has the higher stability than crude extract against 60 minutes irradiation at room temperature. Kinetics degradation of chlorophyll a was based on second order while crude extract based on first order. Chlorophyll a and crude extract have recovery ability. The results also showed that antioxidant activity of chlorophyll a was increased after 60 minutes of irradiation.
Co-Authors Hari Kristopo Leenawaty Limantara