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All Journal AL KAUNIYAH AL-HAYAT: Journal of Biology and Applied Biology BioEksakta : Jurnal Ilmiah Biologi Unsoed Journal of Applied Geology
Dwiana Muflihah Yulianti
Fakultas Biologi Universitas Jenderal Soedirman Purwokerto
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Veterinary

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Karakteristik dan Potensi Enzimatis Bakteri Asal Tanah Sampah Dapur dan Kotoran Ternak sebagai Kandidat Agen Biodegradasi Sampah Organik Taruna Dwi Satwika; Dwiana Muflihah Yulianti; Arif Rahman Hikam
Al-Hayat: Journal of Biology and Applied Biology Vol 4, No 1 (2021)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v4i1.7013

Abstract

Composting is an alternative for recycling organic waste. Microorganisms that can degrade the components of organic waste are an essential part of composting. Isolation and selection of bacteria with the ability to degrade the elements of organic waste are the first steps to obtain an organic waste degrading agent. This study aimed to determine the characteristics and the enzymatic potential (cellulolytic, amylolytic, and proteolytic) of bacteria isolated from soil, cow dung and kitchen waste as candidates for organic waste degradation agents. The research begins with sampling and isolation of bacteria. The isolates obtained were tested qualitatively for their amylolytic, cellulolytic and proteolytic activities. Furthermore, bacterial isolates were characterized morphologically and biochemically. A total of 30 bacterial isolates were isolated from soil samples, cow dung and kitchen waste. The screening result showed that 6 isolates had amylolytic activity, 7 isolates had cellulolytic activity and 3 isolates had proteolytic activity. These bacterial isolates showed various morphological and biochemical characteristics. In general, soil samples, kitchen waste and cow dung contain potential bacteria as organic waste degrading agents
Diversitas dan Potensi Jamur Lignolitik Asal Seresah Daun Arif Rahman Hikam; Dwiana Muflihah Yulianti; Rhevi Raditya Ginanjar
Al-Hayat: Journal of Biology and Applied Biology Vol 4, No 1 (2021)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v4i1.7017

Abstract

One of the biological agents in the biodegradation of organic waste is fungi. Some fungi have the Lignase enzyme, which can degrade Lignin (one of the main organic waste constituents) into simple sugars. The diversity and potential of organic waste degrading fungi still need much research to obtain potential types of fungi that can be developed in organic waste management. This study aimed to determine the diversity and potential of lignolytic fungi in the biodegradation of organic waste. The research was conducted using a descriptive method to take leaf and soil litter samples, isolate and purify fungi, and test lignolytic potential. Based on the isolation results from leaf litter samples, 16 types of fungal isolates were obtained. The lignolytic potential test was carried out using the Bavendamm method. In screening for potential lignolytic ability using the bavendamm method, 7 isolates were found to be positive. The highest lignolytic activity ratio was SR4BD isolate with a ratio value of 1.9. The results showed that the SR4BD isolate was Fusarium sp.
Identifikasi dan UJi Potensi Amilolitik Isolat Jamur Pendegradasi Sampah Organik Insaaniy Mahdiyatul Haqq; Ratna Stia Dewi; Aris Mumpuni; Arif Rahman Hikam; Dwiana Muflihah Yulianti
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4748

Abstract

Sampah organik merupakan sampah yang tersusun dari senyawa organik. Penumpukan sampah organik cukup menjadi masalah serius. Jamur memiliki peran penting untuk mendegradasi sampah organik dalam proses pengomposan. Biodegradasi sampah organik berkaitan erat dengan kemampuan jamur dalam menghidrolisis senyawa amilum. Tujuan dari penelitian ini yaitu mengetahui isolat jamur pendegradasi amilum yang diperoleh dari sampah organik dan mengetahui potensi amilolitik dari isolat jamur tersebut. Penelitian ini dilakukan dengan metode survei dan eksperimental . Sampel sampah dapur yang terdiri atas sisa makanan dan bahan organik lainnya diambil dari rumah penduduk yang terletak di Kelurahan Bancarkembar, Bobosan, Grendeng, Karangwangkal, Pabuaran, Purwanegara, dan Sumampir. Tes screening menggunakan medium Starch Agar untuk mengetahui potensi amilolitik dari isolat jamur. Hasil menunjukkan terdapat delapan isolat jamur yang berpotensi dalam mendegradasi amilum. Sebanyak enam isolat jamur yang memiliki indeks amilolitik terbaik dengan nilai IE ≥ 1 teridentifikasi sebagai isolat Fusarium sp., Aspergilus sp., dan Penicillium sp. Selanjutnya untuk mengetahui aktivitas amilolitik secara kuantitatif dilakukan dengan metode DNS melalui pengukuran kadar glukosa. Isolat jamur Fusarium sp. memiliki aktivitas degradasi amilum yang paling tinggi dengan rata-rata kadar glukosa dari medium Fusarium sp. sebanyak 3.568,63 ppm
Chromium Precipitation Activity and Molecular Characterization of Sulfate-Reducing Bacteria Endah Retnaningrum; Dwiana Muflihah Yulianti; Wahyu Wilopo
Journal of Applied Geology Vol 4, No 1 (2019)
Publisher : Geological Engineering Department Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.397 KB) | DOI: 10.22146/jag.48737

Abstract

Chromium is one of the metals used in many areas of industry., However, chromium is toxic to organisms when present in large quantities in the environment. One of the method for treatment of hazardous waste containing chromium in the aquatic environment can be removed by bioremediation using sulfate-reducing bacteria (SRB). Therefore, the purpose of this research were to analyze the chromium precipitation activity of sulfate-reducing bacteria isolated from sulfate reducing bioreactor and its molecular identification using 16S rRNA gene sequences. The result observed that the isolate of sulfate-reducing bacteria (KGP1 strain) has chromium tolerancy ability up to 5 ppm. It also showed that the strain KGP1 could precipitate chromium up to 0.141 ppm (79 %) on 5 days incubation. Based on 16S rRNA gene sequences, this strain identified as Desulfovibrio aerotolerans.
Potensi Isolat Bakteri Selulolitik Toleran Panas Asal Tanah, Sampah Dapur, dan Kotoran Sapi Dalam Biodegradasi Serasah Daun Taruna Dwi Satwika; Hendro Pramono; Dwiana Muflihah Yulianti
Al-Kauniyah: Jurnal Biologi Vol 16, No 2 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i2.25616

Abstract

 AbstrakBakteri selulolitik memainkan peranan penting dalam biodegradasi komponen selulosa pada sampah organik. Namun, proses pengomposan umumnya melewati fase termofilik (suhu mencapai 55 °C), sehingga tidak semua bakteri dapat bertahan. Sebanyak delapan isolat bakteri selulolitik telah berhasil diisolasi dari tanah, sampah dapur, dan kotoran sapi. Namun, isolat-isolat tersebut belum diketahui aktivitas selulolitiknya pada suhu tinggi dan kemampuannya dalam mendegradasi biomassa serasah daun. Tujuan penelitian ini adalah mengetahui aktivitas selulolitik isolat bakteri pada suhu tinggi secara kualitatif, mengetahui aktivitas selulolitik isolat bakteri secara kuantitatif, dan mengetahui kemampuan isolat bakteri selulolitik dalam mendegradasi biomassa serasah daun. Penelitian dilakukan dengan tahapan peremajaan isolat, skrining kualitatif aktivitas selulolitik isolat pada suhu ruangan, 45 °C dan 55 °C, skrining kuantitatif aktivitas selulolitik isolat, dan uji degradasi biomassa serasah daun. Sebanyak 6 dari 8 isolat bakteri menunjukkan aktivitas selulolitik pada medium Carboxy Methyl Celullose (CMC) Agar pada suhu 55 °C. Berdasarkan uji aktivitas enzim secara kuantitatif, 3 isolat (KS1, KS4, dan SD5) dengan aktivitas enzim tertinggi terpilih untuk pengujian degradasi serasah daun dan menunjukkan rata-rata aktivitas enzim secara berurutan 0,0074 UI/mL; 0,0080 UI/mL; 0,0159 UI/mL. Ketiga isolat mampu mempercepat proses degradasi serasah daun dan berpotensi sebagai agen pengomposan.AbstractHowever, the composting process generally passes through a thermophilic phase (55 °C), so that not all bacteria can survive. A total of 8 isolates of cellulolytic bacteria isolated from soil, kitchen waste, and cow dung have not yet known their cellulolytic activity at high temperatures and their ability to degrade leaf litter biomass. This study aimed to determine the cellulolytic activity of bacterial isolates at high temperatures qualitatively, to determine the cellulolytic activity of bacterial isolates quantitatively, and to determine the ability of these isolates to degrade leaf litter biomass. The research was carried out by reculture isolates; qualitative screening of isolate cellulolytic activity at room temperature, 45 °C and 55 °C; quantitative screening of isolate cellulolytic activity; and leaf litter biomass degradation test. Six of eight bacterial isolates showed cellulolytic activity on Carboxy Methyl Celullose (CMC) Agar medium at 55 °C. Three isolates (KS1, KS4, and SD5) with the highest enzyme activity were selected for the leaf litter degradation test and showed an average enzyme activity of 0.0074 UI/mL; 0.0080 UI/mL; 0.0159 UI/mL, respectively. The three isolates were able to accelerate the degradation process of leaf litter and have potential as composting agents.
Co-Authors Arif Rahman Hikam Aris Mumpuni Endah Retnaningrum Hendro Pramono Insaaniy Mahdiyatul Haqq Ratna Stia Dewi Rhevi Raditya Ginanjar Taruna Dwi Satwika Wahyu Wilopo