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W. Tilaar
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Agriculture, Biological Sciences & Forestry

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INDUKSI KALUS DAN TUNAS DARI EKSPLAN PUCUK BROKOLI (Brassica oleracea L. sub var. italica Planch) PADA MEDIUM MS YANG DIBERIKAN NAA DAN BAP Tilaar, W.; Tulung, S.
EUGENIA Vol 19, No 1 (2013)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.19.1.2013.8377

Abstract

ABSTRACT   The research aimed was to know the combination effect of  NAA and BAP to callus and shoots induction of the explants bud of broccoli on Murashige and Skoog Medium. The research design used Completed Randomized Design in Factorial, which consisted of two treatments combination, i.e: NAA 0; 0,1 ; 1 ppm and BAP 0 ; 1; 2; 3; 4; 5; ppm. Each treatmen was repeated in three times.  The observed variables were time of callus formation, time of shoot formation, high of shoot, and  total shoot added to MS: Analisys of Varians. The result showed that combination of NAA  and BAP influenced significantly the shoot height and the time of shoot formation however not to the total shoot of explant bud. Combination of NAA 1 ppm and BAP 3 ppm was excellent for height shoot however for the number of shoot was combination of NAA 0,1 ppm and BAP 5 ppm, and the time of shoot was on BAP 2 ppm. Callus was not formed on combination of NAA and BAP. Keywords : broccoli, NAA and BAP, MS medium ABSTRAK   Tujuan penelitian ini adalah untuk mengetahui pengaruh kombinasi NAA dan BAP terhadap induksi kalus dan tunas dari eksplan pucuk  brokoli yang ditumbuhkan pada media MS. Rancangan yang digunakan adalah Rancangan Faktorial dalam Rancangan Acak Lengkap terdiri dari dua kombinasi perlakuan yaitu  NAA  dengan konsentrasi 0 ; 0,1 ; 1 ppm dan BAP   0   ; 1 ;  ; 2  ; 3  ;  4  : 5 ppm. Setiap perlakuan diulang tiga kali. Variabel yang diamati : waktu terbentuk kalus, waktu terbentuk tunas, tinggi tunas, dan jumlah tunas. Analisis yang digunakan yaitu Analisis Ragam. Hasil penelitian menunjukan bahwa kombinasi NAA dengan BAP yang ditambahkan ke media MS,  berbeda nyata pengaruhnya terhadap tinggi tunas dan waktu bertunas tetapi tidak berbeda pengaruhnya terhadap jumlah tunas dari eksplan pucuk. Kombinasi terbaik untuk tinggi tunas adalah  NAA 1 ppm dengan BAP 3 ppm, sedangkan pada jumlah tunas adalah NAA 0,1 ppm dengan BAP 5 ppm, dan waktu berbertunas adalah pada BAP 2 ppm. Kalus tidak terbentuk pada kombinasi perlakuan NAA dan BAP. Kata kunci : brokoli, NAA dan BAP, Medium MS
PERSEPSI DAN PARTISIPASI MASYARAKAT DALAM UPAYA REHABILITASI HUTAN DAN LAHAN DI KABUPATEN BOLAANG MONGONDOW TIMUR Mamuko, Frida; Walangitan, H.; Tilaar, W.
EUGENIA Vol 22, No 2 (2016)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.22.2.2016.12959

Abstract

ABSTRAC The aim of this research are; to describe community perception and participation; analyze social and economy factors that influence perception and participation of community of the land and forest rehabilitation programme in East Bolaang Mongondow District. This research was conducted using survay analysis approach. Furthermore,analysis of perception and participation using Likert Scale. Survay was conducted to 102 respondents in 3 Sub Districts inculuding 7 villages in East Bolaang Mongondow District. The results of the research shows that perception level of respondents to the forrest and its function are 48% highly perception, medium perception 45.1 %, and 6.9 % low perception. Furthermore, perception level of respondents to the land and rehabilitation programme are 51% highly perception, medium perception 35%, and 10% respondents low perception. Rank Spearman Correlation analysis shows that perception level influenced by the education level.  Analysis results of the participation level shows that 36.27 % highly perception, 34.31 % medium perception, and 29.41 % low perception. Rank Spearman Correlation analysis also shows that participation level influenced by the education level of the respondents. Keywords : perception, participation, land rehabilitation
PERBANYAKAN IN VITRO PISANG BARANGAN (Musa paradisiacal Var. Sapientum L.) PADA MEDIA MURASHIGE DAN SKOOG DENGAN PENAMBAHAN BENZYLAMINOPURIN Tilaar, W.; Sompotan, saartje
EUGENIA Vol 13, No 2 (2007)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.13.2.2007.7194

Abstract

ABSTRACT Tilaar, W. and S. Sompotan. 2007. Multiplication in vitro of Banana Crop (Musa paradisiacal var. Sapientum L.) in Murashige and Skoog Medium With Suplemented Benzyleaminopurine. Eugenia 13 (2):127-131.   The objectives of this research was to examine in vitro multiplication of Banana in Murashige and Skoog medium by supplementing Benzyleamynopurine. This research was conducted in May-August 2006 at Laboratorium Biotechnology Faculty of Agriculture Sam Ratulangi. A Randomized complete design was use with four replication on each treatment. The treatments were 0; 7,5; 10 and 12,5 ppm. The variables of observed were total shoot, shoot height, and total leaves. The data was analysis using analysis of variance. The result showed that there significant differences on variable total shoot and leaves total. However, there was not significant difference on shoot height.   Keywords: Multiplication in vitro, Murashige and Skoog Medium, Benzylaminopurin, Musa paradisiaca
INDUKSI TUNAS DARI NODUL KRISAN KULO DALAM MEDIA MURASHIGE DAN SKOOG YANG DIBERI SITOKININ Tilaar, W.; Rantung, J.; Tulung, S.
EUGENIA Vol 21, No 2 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.21.2.2015.9713

Abstract

ABSTRACT The kulo chrysanthemum variety is a North Sulawesi local variety characterized by large white flowers.  The new variety was introduced barely two years ago, yet it has gained considerable popularity among flower farmers in Tomohon City as well as in Manado, Tondano, and around the Minahasa and North Minahasa regencies. The growing interest in this particular variety has increased the demand for nursery stock resulting in a shortage of supply.  The conventional plantlet propagation method i.e. one seed to grow one plant, is deemed very time consuming and has poor plantlet yield.   It is therefore necessary to explore other plantlet propagation methods that can produce high yield in a relatively short period of time. One of the best techniques for plantlet propagation is tissue culture.  The success of tissue culture in producing high plantlet yield is determined by the use of appropriate plant growth regulators. Accurate concentrations of growth regulators are vital in achieving an optimum yield.  Consequently, determining the exact concentrations is a major issue in the tissue culture method.    Cytokinins are commonly used as growth regulators in shoot and plantlet propagations.  However, different types of cytokinins may have different effects on shoot generation from the nodal explants used in particular in vitro propagations.  The kulo chrysanthemum variety has never been propagated using the nodal explant tissue culture technique and may respond differently than other varieties.  Therefore, it is essential to determine which types of cytokinins are most suited to stimulate its shoot and plantlet propagations. Keywords:  propagation, chrysanthemum nodes, murashige, skoog, BAP, kinetin, and NAA
PENGGUNAAN ZAT PENGATUR TUMBUH PADA PADI SULUTTAN UNSRAT 1 DAN SULUTTAN UNSRAT 2 Polii-Mandang, Jeany Sh.; Doodoh, Beatrix; Tilaar, W.
EUGENIA Vol 26, No 1 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.26.1.2020.34429

Abstract

This research aims to discover the effects of Gibberellin, Kinetin, IAA, and Paclobutrazol growth regulators towards the growth and yield of suluttan unsrat 1 (SU1) and suluttan unsrat 2 (SU2) rice varieties. This research was done using split plots design with SU1 and SU2 varieties being the main plots and the subplots: Z0 (without plant growth regulator (PGR)); ZK (kinetin treatment); ZG (Giberellin treatment); ZI(IAA treatment); ZP (Paclobutrazol treatment).  Each plot was replicated four times. Results show that there is no interaction between varieties and PGR treatment in temrs of growth variables, yield variables, and yield of SU1 and SU2. Gibberellin spray treatment in the beginning of reproductive stage increased plant height during harvest phase and length of rice panicle, but not increase grains per panicle. Individual treatments of IAA, Kinetin, Paclobutrazol growth regulators did not affect growth, yield, of SU1 and SU2. The timing, dosage, and frequency of PGR applications maybe was not yet optimal and did not increase the yield of SU1 and SU2 varieties.
Co-Authors Doodoh, Beatrix Frida Mamuko, Frida H. Walangitan, H. J. Rantung, J. Polii-Mandang, Jeany Sh. S. Tulung, S. saartje Sompotan