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S. Tulung, S.
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Agriculture, Biological Sciences & Forestry

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INDUKSI KALUS DAN TUNAS DARI EKSPLAN PUCUK BROKOLI (Brassica oleracea L. sub var. italica Planch) PADA MEDIUM MS YANG DIBERIKAN NAA DAN BAP Tilaar, W.; Tulung, S.
EUGENIA Vol 19, No 1 (2013)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.19.1.2013.8377

Abstract

ABSTRACT   The research aimed was to know the combination effect of  NAA and BAP to callus and shoots induction of the explants bud of broccoli on Murashige and Skoog Medium. The research design used Completed Randomized Design in Factorial, which consisted of two treatments combination, i.e: NAA 0; 0,1 ; 1 ppm and BAP 0 ; 1; 2; 3; 4; 5; ppm. Each treatmen was repeated in three times.  The observed variables were time of callus formation, time of shoot formation, high of shoot, and  total shoot added to MS: Analisys of Varians. The result showed that combination of NAA  and BAP influenced significantly the shoot height and the time of shoot formation however not to the total shoot of explant bud. Combination of NAA 1 ppm and BAP 3 ppm was excellent for height shoot however for the number of shoot was combination of NAA 0,1 ppm and BAP 5 ppm, and the time of shoot was on BAP 2 ppm. Callus was not formed on combination of NAA and BAP. Keywords : broccoli, NAA and BAP, MS medium ABSTRAK   Tujuan penelitian ini adalah untuk mengetahui pengaruh kombinasi NAA dan BAP terhadap induksi kalus dan tunas dari eksplan pucuk  brokoli yang ditumbuhkan pada media MS. Rancangan yang digunakan adalah Rancangan Faktorial dalam Rancangan Acak Lengkap terdiri dari dua kombinasi perlakuan yaitu  NAA  dengan konsentrasi 0 ; 0,1 ; 1 ppm dan BAP   0   ; 1 ;  ; 2  ; 3  ;  4  : 5 ppm. Setiap perlakuan diulang tiga kali. Variabel yang diamati : waktu terbentuk kalus, waktu terbentuk tunas, tinggi tunas, dan jumlah tunas. Analisis yang digunakan yaitu Analisis Ragam. Hasil penelitian menunjukan bahwa kombinasi NAA dengan BAP yang ditambahkan ke media MS,  berbeda nyata pengaruhnya terhadap tinggi tunas dan waktu bertunas tetapi tidak berbeda pengaruhnya terhadap jumlah tunas dari eksplan pucuk. Kombinasi terbaik untuk tinggi tunas adalah  NAA 1 ppm dengan BAP 3 ppm, sedangkan pada jumlah tunas adalah NAA 0,1 ppm dengan BAP 5 ppm, dan waktu berbertunas adalah pada BAP 2 ppm. Kalus tidak terbentuk pada kombinasi perlakuan NAA dan BAP. Kata kunci : brokoli, NAA dan BAP, Medium MS
INDUKSI TUNAS DARI NODUL KRISAN KULO DALAM MEDIA MURASHIGE DAN SKOOG YANG DIBERI SITOKININ Tilaar, W.; Rantung, J.; Tulung, S.
EUGENIA Vol 21, No 2 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.21.2.2015.9713

Abstract

ABSTRACT The kulo chrysanthemum variety is a North Sulawesi local variety characterized by large white flowers.  The new variety was introduced barely two years ago, yet it has gained considerable popularity among flower farmers in Tomohon City as well as in Manado, Tondano, and around the Minahasa and North Minahasa regencies. The growing interest in this particular variety has increased the demand for nursery stock resulting in a shortage of supply.  The conventional plantlet propagation method i.e. one seed to grow one plant, is deemed very time consuming and has poor plantlet yield.   It is therefore necessary to explore other plantlet propagation methods that can produce high yield in a relatively short period of time. One of the best techniques for plantlet propagation is tissue culture.  The success of tissue culture in producing high plantlet yield is determined by the use of appropriate plant growth regulators. Accurate concentrations of growth regulators are vital in achieving an optimum yield.  Consequently, determining the exact concentrations is a major issue in the tissue culture method.    Cytokinins are commonly used as growth regulators in shoot and plantlet propagations.  However, different types of cytokinins may have different effects on shoot generation from the nodal explants used in particular in vitro propagations.  The kulo chrysanthemum variety has never been propagated using the nodal explant tissue culture technique and may respond differently than other varieties.  Therefore, it is essential to determine which types of cytokinins are most suited to stimulate its shoot and plantlet propagations. Keywords:  propagation, chrysanthemum nodes, murashige, skoog, BAP, kinetin, and NAA
PENENTUAN DOSIS FORMULASI PUPUK ORGANIK UNTUK EFISIENSI PENGGUNAAN PHONSKA PADA TANAMAN JAGUNG MANIS (Zea mays sacharata Sturt.) Tulungen, A. G.; Tumewu, P.; Montolalu, M.; Rantung, John L.; Tulung, S.
EUGENIA Vol 25, No 2 (2019)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.25.2.2019.33807

Abstract

The use of organic fertilizer provides soil with macronutrients, micronutrients, and organic acids which improve the long-term physical, chemical, and biological fertility of the soil. This research aims to: 1) Increase physical and chemical soil fertility, 2) Acquire the interaction between organic fertilizer and Ponska fertilizer dosage on sweet corn crops, 3) Acquire the most efficient dosage of PONSKA  fertilizer on sweet corn, and 4) disseminate research results through journal articles and directly to farmers.  This research will increase the efficiency of NPK Ponska chemical fertilizers by utilizing waste from around the farm and lakes to formulate organic fertilizer to apply on sweet corn crops and as a reference to teaching material. Factorial research with two factors was designed using a randomized block design. Factor I (A) = Organic fertilizer formulation, comprising of A1 = 20 ton/ha, A2 = 30 ton/ha, and A3 = 40 ton/ha.  Factor II (B) =  Phonska fertilizer,  comprising of B1 = 25 % Ponska, and B2 = 50% Ponska. Every treatment was replicated four times, yielding 24 research plots. Response variables observed were: Plant height, number of leaves, dry weight of the plant. Data were analyzed using ANOVA, followed by Tukey's honest significance difference test (alpha = 0.05) if significant differences were found. Results showed that the interaction between organic and Phonska fertilizer formulations had significant effects on plant height. A dosage of 40 ton/ha of organic fertilizer can decrease the use of phonska fertilizer by 75% based on the height of sweet corn plants.
Co-Authors J. Rantung, J. John L. Rantung, John L. Montolalu, M. Tulungen, Antje Grace Tumewu, P. W. Tilaar