Garuda - Garba Rujukan Digital

The effects of proline, carnitine on the viability of sperm stored at 5oC (chilled semen) Situmorang, Polmer; Triwulanningsih, E; Lubis, A; Caroline, W; Sugiarti, T
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 1 (2001)
Publisher : Indonesian Animal Sciences Society

An experiment was carried out to evaluate the addition of proline, carnitine in Tris-extender on the viability of bull sperm following storage at 5oC. Semen was collected by means of artificial vagina (AV), diluted in Tris-extender containing 5% V/V egg yolk (EY) and 4% V/V glycerol to get a final concentration of 50 million sperms/ml. Diluted semen cooled to 5oC for 45 minute and stored at those temperature for 1, 3, 10, and 13 days. In the first activity the addition of 15, 30 and 60 mM carnitine in Tris-extender while in the second activity the inclusion of 15, 30, and 60 mM proline on the viability of sperm was investigated. Addition of carnitine to Tris-extender signifinatly increase (P<0.05) the viability of sperm after storage for more than 3 days. At 3 days of storage, the mean %M and %L were 27.3, 38.8, 33.5, 53.0, 31.8, 47.0, and 30.5, 46.8 for control 15, 30, and 60 mM carnitine respectively. The similar results was obtained for 7 days of storage where the mean %M and %L for control (12.5 and 27.3) was significantly lower (P<0.05) than those 15, 30, and 60 mM carnitine (15.0, 33.5, 18.8, 36.5, 17.5, 36.3). The superiority of carnitine was maintained for 10 days of storage, where the mean %L were 23.5, 28.8, 31.5, and 30.3 for control; 15; 30; and 60 mM respectively. There was no any significant within concentration of carnitine tested (15 to 60 mM).The condition of apical ridge was not significantly affected by carnitine. In the second activity, inclusion of proline to Trisextender statistically (P<0.05) improved the viability of sperm after storage for 7 and 13 days. After 7 days of storage the mean %M and %L were 31.4, 36.4, 38.8, 40.4, 36.6, 42.7, and 34.8, 43.3 for control; 15, 30, and 60 mM proline respectively. The significant effects of proline was remain for 13 days of storage where the mean %M and % L were 24.6, 32.9, 28.6, 37.5, 29.1, 39.8, and 30.1, 37.3 for control; 15, 30, and 60 mM proline respectively. There was no significant difference within the concentration of proline. Condition of apical ridge was not significantly affected by proline. Â Key words: Sperm, viability, carnitine, proline

The effect of cryoprotectant and equilibration period on quality and fertility of duck and muscovy sperm Setioko, A.R; Situmorang, P; Triwulanningsih, E; Sugiarti, T; Kusumaningrum, D.A
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 4 (2002)
Publisher : Indonesian Animal Sciences Society

The study was conducted to evaluate the effect of cryoprotectant and equilibration period on quality and fertility of duck and muscovy spermatozoa. Semen collected from Alabio and muscovy drakes was diluted using three different cryoprotectants:glycerol, DMSO and DMF, thereafter the semen was equilibrated 50C for 15; 30 and 60 minutes then frozen and stored in liquid nitrogen, designed by factorial 3 x 3. After thawing, semen sample was investigated on the motility and mortality rate. The best cryoprotectant and equilibration period was used in fertilization test. Duration of fertility was calculated from the second day after insemination until the last fertile egg, and the percent of fertility was calculated from the second day until the forth day after insemination. The use of cryoprotectant significantly affected sperm motility after freezing. The use of glycerol as a cryoprotectant was the lowest (P<0.05) compared to DMSO and DMF. Similarly, duck sperm motility after being freezed with glycerol, DMF and DMSO were 9.02; 21.75, and 32.86%, and for muscovy sperm motility were 11.78; 32.45 and 34.92% respectively. The percentage of live sperm for duck were 23.84; 40.14 and 42.20, while for muscovy were 29.26; 53.06 and 51.80 respectively after being freezed with glycerol, DMF and DMSO. Equilibration period did not affect the percentage of live sperm after freezing. Results of this study showed that duration of fertility of Alabio duck after being inseminated with fresh drake semen was longest compared to that of insemination using fresh muscovy semen, frozen drake semen and frozen muscovy semen (4.96 vs 3.5; 2.4 and 1.5 days respectively). Results from this study clearly indicated that preservation of sperm reduced the quality of spermatozoa. It is suggested that freezing technique of both duck and Muscovy sperm could be conducted using DMF or DMSO as a cryoprotectant with the equilibration period between 15 to 60 minutes. Â Key words: Sperm, cryoprotectant, fertility, AI, duck, muscovy

Effect of cryoprotectant and its level to survivability of drake semen D.A, Kusumaningrum; Situmorang, P; Setioko, A.R; Sugiarti, T; Triwulanningsih, E; Sianturi, R.G
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 4 (2002)
Publisher : Indonesian Animal Sciences Society

This study was conducted at Laboratory Reproduction of Physiology, Research Institute of Animal Production. The experiment was factorial design with two kinds of cryoprotectant (DMF and DMA) as the first factor and two levels of them (7 and 9%) as the second factor. This study was invented to determine the effect of cryoprotectant and its level to survivability of drake semen. Sperm was collected from fifteen drakes two times per week using artivicial vagina. Only the best quality of sperm was used in this study. Collected sperm was diluted in medium to get concentration of 400 x 106 per ml. Equilibrated at 50C in mini straw (0.25 ml) for 60 minutes, then kept them up 8 cm above the LN2 for 4 minutes before plunged in LN2. Parameters measured of this study were survability of drake semen after diluted, at 50C and after freezing-thawing at 350C for 30 seconds. Result of this study showed that percentage of motility and percentage of live sperm were significant different (P<0.05) between DMA (33.24 and 42.03) and DMF (25.82 and 34.30). Level of cryoprotectant (7 and 9%) were not significant different. Based on this study, using DMA as cryoprotectan with 7% in medium was better than that of DMF. Â Key words: Cryoprotectan, survivability, drake sperm

PENGARUH FORMALIN DAN pH TERHADAP DAYA HIDUP SPERMATOZOA SAPI YANG DISIMPAN PADA SUHU RUANGAN MAUPUN 5Â°C Situmorang, Polmer; Triwulanningsih, E; Sugiarti, T; Kusumaningrum, DA; Sianturi, RG
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The study was conducted in Research Institute for Animal Production (RIAP)-Ciawi. Semen was collected from 3 bulls twice a week by artificial vagina (AV), diluted in Tris buffer (control) and containing of 0.0625% formalin, reducing pH to 5.5 or their combination to temporally inhibit the motility of sperm.Diluted semen were store at ambient temperature for 0, 3 and 24 hours or at 5Â°C for 0, 3 and 7 days.Reactivation of motility was performed by dilution of formalin and bring the pH to 7.0. In ambient temperature,formalin and pH significantly (p<0.01) inhibit the motility of sperm, but these inhibition was reversible. The percentage of motility was significantly higher(p<0.01) in formalin than in pH 5.5 or control, following 24 hours of storage periode. The inhibition effect of combination between formalin and pH was non permanent. It is show that the inhibition effect of treatments was permanent for semen stored at 5Â°C.

The effect of cryoprotectant and equilibration period on quality and fertility of duck and muscovy sperm A.R Setioko; P Situmorang; E Triwulanningsih; T Sugiarti; D.A Kusumaningrum
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 4 (2002): DECEMBER 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The study was conducted to evaluate the effect of cryoprotectant and equilibration period on quality and fertility of duck and muscovy spermatozoa. Semen collected from Alabio and muscovy drakes was diluted using three different cryoprotectants:glycerol, DMSO and DMF, thereafter the semen was equilibrated 50C for 15; 30 and 60 minutes then frozen and stored in liquid nitrogen, designed by factorial 3 x 3. After thawing, semen sample was investigated on the motility and mortality rate. The best cryoprotectant and equilibration period was used in fertilization test. Duration of fertility was calculated from the second day after insemination until the last fertile egg, and the percent of fertility was calculated from the second day until the forth day after insemination. The use of cryoprotectant significantly affected sperm motility after freezing. The use of glycerol as a cryoprotectant was the lowest (P<0.05) compared to DMSO and DMF. Similarly, duck sperm motility after being freezed with glycerol, DMF and DMSO were 9.02; 21.75, and 32.86%, and for muscovy sperm motility were 11.78; 32.45 and 34.92% respectively. The percentage of live sperm for duck were 23.84; 40.14 and 42.20, while for muscovy were 29.26; 53.06 and 51.80 respectively after being freezed with glycerol, DMF and DMSO. Equilibration period did not affect the percentage of live sperm after freezing. Results of this study showed that duration of fertility of Alabio duck after being inseminated with fresh drake semen was longest compared to that of insemination using fresh muscovy semen, frozen drake semen and frozen muscovy semen (4.96 vs 3.5; 2.4 and 1.5 days respectively). Results from this study clearly indicated that preservation of sperm reduced the quality of spermatozoa. It is suggested that freezing technique of both duck and Muscovy sperm could be conducted using DMF or DMSO as a cryoprotectant with the equilibration period between 15 to 60 minutes. Key words: Sperm, cryoprotectant, fertility, AI, duck, muscovy

Effect of cryoprotectant and its level to survivability of drake semen Kusumaningrum D.A; P Situmorang; A.R Setioko; T Sugiarti; E Triwulanningsih; R.G Sianturi
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 4 (2002): DECEMBER 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

This study was conducted at Laboratory Reproduction of Physiology, Research Institute of Animal Production. The experiment was factorial design with two kinds of cryoprotectant (DMF and DMA) as the first factor and two levels of them (7 and 9%) as the second factor. This study was invented to determine the effect of cryoprotectant and its level to survivability of drake semen. Sperm was collected from fifteen drakes two times per week using artivicial vagina. Only the best quality of sperm was used in this study. Collected sperm was diluted in medium to get concentration of 400 x 106 per ml. Equilibrated at 50C in mini straw (0.25 ml) for 60 minutes, then kept them up 8 cm above the LN2 for 4 minutes before plunged in LN2. Parameters measured of this study were survability of drake semen after diluted, at 50C and after freezing-thawing at 350C for 30 seconds. Result of this study showed that percentage of motility and percentage of live sperm were significant different (P<0.05) between DMA (33.24 and 42.03) and DMF (25.82 and 34.30). Level of cryoprotectant (7 and 9%) were not significant different. Based on this study, using DMA as cryoprotectan with 7% in medium was better than that of DMF. Key words: Cryoprotectan, survivability, drake sperm

The effects of proline, carnitine on the viability of sperm stored at 5oC (chilled semen) Polmer Situmorang; E Triwulanningsih; A Lubis; W Caroline; T Sugiarti
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 1 (2001): MARCH 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

An experiment was carried out to evaluate the addition of proline, carnitine in Tris-extender on the viability of bull sperm following storage at 5oC. Semen was collected by means of artificial vagina (AV), diluted in Tris-extender containing 5% V/V egg yolk (EY) and 4% V/V glycerol to get a final concentration of 50 million sperms/ml. Diluted semen cooled to 5oC for 45 minute and stored at those temperature for 1, 3, 10, and 13 days. In the first activity the addition of 15, 30 and 60 mM carnitine in Tris-extender while in the second activity the inclusion of 15, 30, and 60 mM proline on the viability of sperm was investigated. Addition of carnitine to Tris-extender signifinatly increase (P<0.05) the viability of sperm after storage for more than 3 days. At 3 days of storage, the mean %M and %L were 27.3, 38.8, 33.5, 53.0, 31.8, 47.0, and 30.5, 46.8 for control 15, 30, and 60 mM carnitine respectively. The similar results was obtained for 7 days of storage where the mean %M and %L for control (12.5 and 27.3) was significantly lower (P<0.05) than those 15, 30, and 60 mM carnitine (15.0, 33.5, 18.8, 36.5, 17.5, 36.3). The superiority of carnitine was maintained for 10 days of storage, where the mean %L were 23.5, 28.8, 31.5, and 30.3 for control; 15; 30; and 60 mM respectively. There was no any significant within concentration of carnitine tested (15 to 60 mM).The condition of apical ridge was not significantly affected by carnitine. In the second activity, inclusion of proline to Trisextender statistically (P<0.05) improved the viability of sperm after storage for 7 and 13 days. After 7 days of storage the mean %M and %L were 31.4, 36.4, 38.8, 40.4, 36.6, 42.7, and 34.8, 43.3 for control; 15, 30, and 60 mM proline respectively. The significant effects of proline was remain for 13 days of storage where the mean %M and % L were 24.6, 32.9, 28.6, 37.5, 29.1, 39.8, and 30.1, 37.3 for control; 15, 30, and 60 mM proline respectively. There was no significant difference within the concentration of proline. Condition of apical ridge was not significantly affected by proline. Key words: Sperm, viability, carnitine, proline

Effect of exogenous glutathione in medium fertilization to improve blastocyst rates of bovine embryos E Triwulanningsih; P Situmorang; I-G Putu; T Sugiarti; A.M Lubis; D.A Kusumaningrum; W Caroline; R.G Sianturi
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 2 (2002): JUNE 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Glutathione (C10H17N3O6S) is a tripeptide (γ-Glu-Cys-Gly) widespread in living organism. Glutathione (GSH) at the 5 mM concentration increased the motility and fertility of frozen-thawed sperm. Intracellulair glutathione improved the cleavage rate and embryo development to the blastocyst rate. Research on in vitro embryos production through the implementation of GSH during IVF (in vitro fertilization) on embryo development has been conducted at the Laboratorium Reproductive of Physiology, Research Institute for Animal Production. Ovaries of beef cattle as source of oocytes were collected from the slaughterhouse in a thermo flask with 350C PBS as medium and transported to the laboratory. The oocytes were fertilized in vitro with selected motile sperm using Percoll gradient (90 and 45%). Ten COCs (cumulus oocytes complexes) were transfered to 44 μl of fertilization medium (mTALP) was performed with either 0; 0.25; 0.50; 0.75 and 1.00 mM of glutathione as treatment A, B, C, D and E respectively, and inseminated with 2 μl of capacitated sperm and added 2 μl of heparin and 2 μl of PHE (consisting of 20 μM penicillamine, 10 μM hypotaurine, 1 μM epinephrine). Incubation between sperm and oocytes in the 5% CO2 incubator and RH 90% in fertilization media (TALP) for 20 hours. All zygotes were cultured in modification of CR1aa medium up to blastocyst and were fed serum 5 μl/50μl medium on day 6. Results of this experiment showed that the effect of concentration of glutathione (0, 0.25; 0.50; 0.75 and 1.00 mM) on fertilization medium to the percentage of cleavage rates were 69.35 + 29.40; 79.07 + 13.17; 67.88 + 10.65; 98.10 + 3.30 and 82.62 + 24.19% not significant different (P>0.05) among treatments A, B, C, D dan E respectively.The precentages of morula and blastocyst for treatment D were 50.45 + 42.64% and 31.28 + 24.28%; while 36.55 + 24.13% and 17.74 + 17.86% for treatment E respectively. Key words: Glutathione, in vitro fertilization, oocytes, cleavage

PENGARUH FORMALIN DAN pH TERHADAP DAYA HIDUP SPERMATOZOA SAPI YANG DISIMPAN PADA SUHU RUANGAN MAUPUN 5°C Polmer Situmorang; E Triwulanningsih; T Sugiarti; DA Kusumaningrum; RG Sianturi
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i3.1063

The study was conducted in Research Institute for Animal Production (RIAP)-Ciawi. Semen was collected from 3 bulls twice a week by artificial vagina (AV), diluted in Tris buffer (control) and containing of 0.0625% formalin, reducing pH to 5.5 or their combination to temporally inhibit the motility of sperm.Diluted semen were store at ambient temperature for 0, 3 and 24 hours or at 5°C for 0, 3 and 7 days.Reactivation of motility was performed by dilution of formalin and bring the pH to 7.0. In ambient temperature,formalin and pH significantly (p<0.01) inhibit the motility of sperm, but these inhibition was reversible. The percentage of motility was significantly higher(p<0.01) in formalin than in pH 5.5 or control, following 24 hours of storage periode. The inhibition effect of combination between formalin and pH was non permanent. It is show that the inhibition effect of treatments was permanent for semen stored at 5°C.

Title

Found 9 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 9 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 9 Documents
Search