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The effects of proline, carnitine on the viability of sperm stored at 5oC (chilled semen) Situmorang, Polmer; Triwulanningsih, E; Lubis, A; Caroline, W; Sugiarti, T
Indonesian Journal of Animal and Veterinary Sciences Vol 6, No 1 (2001)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.85 KB) | DOI: 10.14334/jitv.v6i1.211

Abstract

An experiment was carried out to evaluate the addition of proline, carnitine in Tris-extender on the viability of bull sperm following storage at 5oC. Semen was collected by means of artificial vagina (AV), diluted in Tris-extender containing 5% V/V egg yolk (EY) and 4% V/V glycerol to get a final concentration of 50 million sperms/ml. Diluted semen cooled to 5oC for 45 minute and stored at those temperature for 1, 3, 10, and 13 days. In the first activity the addition of 15, 30 and 60 mM carnitine in Tris-extender while in the second activity the inclusion of 15, 30, and 60 mM proline on the viability of sperm was investigated. Addition of carnitine to Tris-extender signifinatly increase (P<0.05) the viability of sperm after storage for more than 3 days. At 3 days of storage, the mean %M and %L were 27.3, 38.8, 33.5, 53.0, 31.8, 47.0, and 30.5, 46.8 for control 15, 30, and 60 mM carnitine respectively. The similar results was obtained for 7 days of storage where the mean %M and %L for control (12.5 and 27.3) was significantly lower (P<0.05) than those 15, 30, and 60 mM carnitine (15.0, 33.5, 18.8, 36.5, 17.5, 36.3). The superiority of carnitine was maintained for 10 days of storage, where the mean %L were 23.5, 28.8, 31.5, and 30.3 for control; 15; 30; and 60 mM respectively. There was no any significant within concentration of carnitine tested (15 to 60 mM).The condition of apical ridge was not significantly affected by carnitine. In the second activity, inclusion of proline to Trisextender statistically (P<0.05) improved the viability of sperm after storage for 7 and 13 days. After 7 days of storage the mean %M and %L were 31.4, 36.4, 38.8, 40.4, 36.6, 42.7, and 34.8, 43.3 for control; 15, 30, and 60 mM proline respectively. The significant effects of proline was remain for 13 days of storage where the mean %M and % L were 24.6, 32.9, 28.6, 37.5, 29.1, 39.8, and 30.1, 37.3 for control; 15, 30, and 60 mM proline respectively. There was no significant difference within the concentration of proline. Condition of apical ridge was not significantly affected by proline.   Key words: Sperm, viability, carnitine, proline
The effects of proline, carnitine on the viability of sperm stored at 5oC (chilled semen) Polmer Situmorang; E Triwulanningsih; A Lubis; W Caroline; T Sugiarti
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 1 (2001): MARCH 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.85 KB) | DOI: 10.14334/jitv.v6i1.211

Abstract

An experiment was carried out to evaluate the addition of proline, carnitine in Tris-extender on the viability of bull sperm following storage at 5oC. Semen was collected by means of artificial vagina (AV), diluted in Tris-extender containing 5% V/V egg yolk (EY) and 4% V/V glycerol to get a final concentration of 50 million sperms/ml. Diluted semen cooled to 5oC for 45 minute and stored at those temperature for 1, 3, 10, and 13 days. In the first activity the addition of 15, 30 and 60 mM carnitine in Tris-extender while in the second activity the inclusion of 15, 30, and 60 mM proline on the viability of sperm was investigated. Addition of carnitine to Tris-extender signifinatly increase (P<0.05) the viability of sperm after storage for more than 3 days. At 3 days of storage, the mean %M and %L were 27.3, 38.8, 33.5, 53.0, 31.8, 47.0, and 30.5, 46.8 for control 15, 30, and 60 mM carnitine respectively. The similar results was obtained for 7 days of storage where the mean %M and %L for control (12.5 and 27.3) was significantly lower (P<0.05) than those 15, 30, and 60 mM carnitine (15.0, 33.5, 18.8, 36.5, 17.5, 36.3). The superiority of carnitine was maintained for 10 days of storage, where the mean %L were 23.5, 28.8, 31.5, and 30.3 for control; 15; 30; and 60 mM respectively. There was no any significant within concentration of carnitine tested (15 to 60 mM).The condition of apical ridge was not significantly affected by carnitine. In the second activity, inclusion of proline to Trisextender statistically (P<0.05) improved the viability of sperm after storage for 7 and 13 days. After 7 days of storage the mean %M and %L were 31.4, 36.4, 38.8, 40.4, 36.6, 42.7, and 34.8, 43.3 for control; 15, 30, and 60 mM proline respectively. The significant effects of proline was remain for 13 days of storage where the mean %M and % L were 24.6, 32.9, 28.6, 37.5, 29.1, 39.8, and 30.1, 37.3 for control; 15, 30, and 60 mM proline respectively. There was no significant difference within the concentration of proline. Condition of apical ridge was not significantly affected by proline.   Key words: Sperm, viability, carnitine, proline
Reduksi suara bising genset menggunakan kotak genset berperedam 3 cm A Suudi*; A Lubis; N Tanti; D Ardimansyah
Prosiding Seminar Nasional Ilmu Teknik Dan Aplikasi Industri Fakultas Teknik Universitas Lampung Vol. 3 (2020)
Publisher : Fakultas Teknik Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (812.085 KB) | DOI: 10.23960/prosidingsinta.v3i.21

Abstract

Kehidupan masyarakat modern sangat tergantung dengan listrik. Kebutuhan listrik di Indonesia disediakan oleh negara melalui Perusahaan Listrik Negara (PLN). Pada saat terjadi gangguan penyediaan listrik oleh PLN, banyak masyarakat menggunakan generator set (genset) sebagai alternatif. Permasalahan yang muncul adalah suara bising yang ditimbulkan genset, karena kebanyakan genset yang dimiliki masyarakat adalah jenis genset rumah tangga. Berdasar hal tersebut pada penelitian ini dilakukan reduksi suara bising genset. Reduksi suara bising genset dilakukan dengan cara merancang dan membuat kotak peredam genset. Kotak peredam genset dirancang bersifat knockdown dan portabel dengan dimensi menyesuaikan jenis genset yang digunakan. Rangka dinding kotak peredam terbuat dari besi hollow ukuran 20x20 mm dengan tebal 3 mm. Dinding kotak dan dinding peredam terbuat dari material triplek. Busa peredam yang digunakan jenis acoustic foam pyramid SPF28 ukuran 50x50 cm tebal 3 cm. Pengujian dilakukan di alam terbuka dengan 4 arah pengukuran. Pada setiap arah ada tiga titik pengukuran masing-masing 1 m, 2 m dan 4 m dari genset. Kebisingan genset pada setiap titik diukur selama satu menit dengan interval pengambilan data setiap 5 detik menggunakan Sound Level Meter (SLM) Bruel & Kjaer Type 2239. Hasil penelitian menunjukkan kotak peredam genset dengan tebal dinding peredam 3 cm mampu mereduksi suara bising genset awal sebesar 23% pada jarak 4 meter dari genset.
Co-Authors A Suudi* D Ardimansyah E Triwulanningsih N Tanti Polmer Situmorang T Sugiarti W Caroline