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Wirnangsi D Uno
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AKTIVITAS LACTOBACILLUS BULGARICUS PADA FERMENTASI SUSU JAGUNG (ZEA MAYS ) DENGAN PENAMBAHAN SUKROSA DAN LAKTOSA Machmud, Nur Afni; Retnowati, Yuliana; Uno, Wirnangsi D
Sainstek VOL 07, NO 02, 2013
Publisher : Sainstek

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Abstract

Penelitian ini bertujuan untuk mengetahui aktivitas bakteri Lactobacillus bulgaricus pada fermentasi susu jagung dengan penambahan sukrosa dan laktosa. Aktivitas bakteri ditentukan melalui pengukuran kadar asam laktat dengan data pendukung jumlah bakteri dan pH lingkungan. Kadar asam laktat dianalisis menggunakan Metode Mann Acid Test. Penelitian didasarkan pada metode eksperimen menggunakan rancangan acak blok dengan 3 perlakuan (susu jagung murni, susu jagung dengan penambahan sukrosa dan susu jagung dengan penambahana laktosa) dengan 9 ulangan. Hipotesis diuji dengan analisis statistik nonparametrik menggunakan kruskall wallis dan untuk menentukan perbedaan pada setiap perlakuan dilanjutkan dengan uji Mann-Whitney. Hasil analisis menunjukkan bahwa penambahan sukrosa dan laktosa tidak berbeda nyata dengan hasil kadar asam laktat 1,624% dan 1,953%. Pada perlakuan fermentasi susu jagung murni berbeda nyata dengan kedua perlakuan tersebut dengan hasil kadar asam laktat yaitu 1,062%.
POTENSI PENGHASILAN HORMON IAA OLEH MIKROBA ENDOFIT AKAR TANAMAN JAGUNG (Zea mays) Retnowati, Yuliana; Uno, Wirnangsi D; Putri, Siti Humairah Eka
Sainstek VOL 06, NO 06, 2012
Publisher : Jurnal Sainstek

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The aim of the study was to obtain an endofityc microorganisms that potential to produce IAA hormone in the root of corn and to analyze the ability to produce IAA by manipulating of the growth medium that used the liquid waste of tofu industry. The method based on descriptive methode that describe the ability of ed endofityc microorganism in IAA hormone producing at the liquid waste of tofu industry. The isolation of endofityc bacterial based on Radu and Kqeen method (2002), then characterization and ion was done. The ability producing IAA hormone test was based on colorimetry method used spectrophotometer on 530 nm of wavelength. The results showed that there were six isolates of endophytic bacteria isolated from the roots of Bisi and Motoro II maize varieties which shows the character of colony morphology and cell-specific forms. The results of measurements of the ability of the hormone IAA in vitro income showed an increase in levels of IAA until the seventh day of incubation. the test results of hormone production on the medium tofu industrial wastewater know precisely a decline compared to in vitro tests and the highest IAA levels achieved at 48 hours of incubation.
Co-Authors Nur Afni Machmud Siti Humairah Eka Putri Yuliana Retnowati