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All Journal Majalah Kedokteran Sriwijaya
Hanna Mutiara
Department of Parasitology Faculty of Medicine University of Lampung
Health Professions Medicine & Pharmacology Nursing Public Health

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PfCRT GENE POLYMORPHISMS IN PLASMODIUM FALCIPARUM ISOLATES FROM MALARIA PATIENTS IN MALARIA ENDEMIC AREAS USING ACT AS STANDARD THERAPY Jhons Fatriyadi Suwandi; Betta Kurniawan; Hanna Mutiara; Aila Karyus
Majalah Kedokteran Sriwijaya Vol 54, No 1 (2022): Majalah Kedokteran Sriwijaya
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/mks.v54i1.15137

Abstract

The pfcrt gene is a biomarker to determine the resistance of Plasmodium falciparum to chloroquine and amodiaquine. When chloroquine was switched to ACT, it is very likely that there will be an increase in wild type strains (pfcrt K76) due to the absence of exposure to chloroquine. Currently chloroquine has not used for malaria treatment. The aims of this study were to identify polymorphisms in the pfcrt gene and phylogenetic analysis of Plasmodium falciparum isolates from malaria patients in Pesawaran Regency, Lampung Province. This research is a laboratory research by analyzing blood samples which are stored biological materials (BBT). DNA isolation was carried out using a DNA isolation kit QIAmp DNA Mini kit from Qiagen, followed by amplification using an appropriate primer. The amplification results were followed by sequencing and then analyzed using Mega 10. The results showed that all samples had mutations in codons 76 (K76T) and 72 (C72S) of the pfcrt gene. Pesawaran isolates were closely related to other isolates. The conclusion of this study is that polymorphisms were found in codons 72 and 76 of the PfCRT gene, although chloroquine has long been abandoned as an antimalarial. The sequenced pesawaran isolates were also related to other isolates. 
Co-Authors Aila Karyus Betta Kurniawan, Betta Jhons Fatriyadi Suwandi