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PERUBAHAN KANDUNGAN ASAM FITAT DAN ASAM AMINO ESENSIAL BAHAN-BAHAN ORGANIK PAKAN YANG DIFERMENTASI RAGI TEMPE Muniroh, Arina; Suja'i, Imam; Wibowo, Aji; Saputra, Henry Kasman Hadi; Yunita, Etyn; Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (996.834 KB) | DOI: 10.29122/jbbi.v8i1.4743

Abstract

Changes in the Contents of Phytic Acid and Essential Amino Acids of Animal Feed Organic Materials Fermented by Tempe Starter The contents of antinutrients and nutrients are important in the selection of feed ingredients, especially for monogastric animals. The aim of this study is to determine the change in the contents of antinutrient phytic acid and nutrient essential amino acid in selected organic materials. Nine organic ingredients of agro-industrial by-products, namely rice bran, coffee skin, cassava bagasse, corn, coconut dreg, soy bean meal, pollard, corn gluten feed (CGF), and copra meal were fermented in solid state using tempeh starter for 48 hours at 30°C. Dense mycelia overgrowing evenly on the top, bottom, and cross-sliced surfaces was observed on rice bran, maize and copra meal substrates. These 3 selected organic materials were then analysed for the content of phytic acid and essential amino acids. The results showed that the lowest decrease (75.80%) in phytic acid content occurred in maize, namely from 18.49 ± 0.41 mg g-1 (before fermentation) to 4.48 ± 0.19 mg g-1 (after fermentation). The highest increase (59%) of total essential amino acids occurred in copra meal, namely from 38,991.89 ± 447.12 mg kg-1 (before fermentation) to 61,816.56 ± 894.24 mg kg-1 (after fermentation). Kandungan antinutrisi dan nutrisi merupakan hal penting dalam pemilihan bahan pakan, terutama untuk hewan monogastrik. Penelitian ini bertujuan mengetahui perubahan kandungan antinutrisi asam fitat dan nutrisi asam amino esensial pada bahan organik tertentu yang terpilih. Sembilan bahan organik yang merupakan hasil samping agroindustri, yakni dedak padi, kulit kopi, onggok, jagung, ampas kelapa, bungkil kedelai (soy bean meal), dedak gandum (pollard), produk samping jagung corn gluten feed (CGF), dan bungkil kopra difermentasi padat menggunakan ragi tempe selama 48 jam pada suhu 30°C. Miselium yang tumbuh subur, padat, dan merata pada permukaan atas, bawah, dan irisan melintang teramati pada dedak padi, jagung, dan bungkil kopra. Tiga bahan organik terpilih ini kemudian dianalisa kandungan asam fitat dan asam amino esensialnya. Hasil menunjukkan bahwa penurunan terbesar (75,80%) kandungan asam fitat terjadi pada jagung, yakni dari 18,49 ± 0,41 mg g-1 (sebelum fermentasi) menjadi 4,48 ± 0,19 mg g-1 (setelah fermentasi). Peningkatan tertinggi (59%) asam amino esensial total terjadi pada bungkil kopra, yakni dari 38.991,89 ± 447,12 mg kg-1 (sebelum fermentasi) menjadi 61.816,56 ± 894,24 mg kg-1 (setelah fermentasi).
Perbandingan Metode CBIA dan FGD dalam Peningkatan Pengetahuan dan Ketepatan Caregiver dalam Upaya Swamedikasi Demam pada Anak Lathifah, Marianti Avi; Susanti, Susanti; Ilham, Much; Wibowo, Aji
Pharmaceutical Sciences and Research
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Self medications which are ofted applied to children are including coughs, colds, flu, and nasal congestion (17.2%), fever (15%), headache (14%), diarrhea and abdominal pain (9%). The results from previous studies showed only 0.4% caregiver of 4-6 years old children in Banyumas Regency did correctly in children’s fever self medication. Aim of this study is to determine the influence of Community Based Interactive Approach (CBIA) and Focus Group Discussion (FGD) methods for improving knowledge and correctness of caregivers in children’s fever self medication with type of research of quasi experimental design with a control group of non random pretest posttest. Results of Wilcoxon test showed that there were significant differences of knowledge statistically before and after treatment using the FGD and CBIA methods with significancy values in FGD group of 0.000 (p < 0.05) and in CBIA group of 0.002 (p < 0.05), while for correctness before and after treatment with FGD and CBIA methods, there were no statistically significant difference in significancy values of correctness for FGD group of 1,000 (p> 0.05) and for the CBIA group of 1,000 (p> 0.05). Results of Mann-Whitney test showed that the obtained significancy value was 0.012 (p <0.05), it can be concluded that there were significant differences between FGD with CBIA groups. The difference of Mean scores in FGD group (2.45) were greater than the CBIA group (0.96), so that the FGD method was better in improving the knowledge compared with CBIA method even though they both gave some improvements.. Fisher’s Exact test results showed that the ​obtained significancy value was 1,000. According to these statistical analysis results, it can be concluded that based on statistical calculatios, there is no significant difference between FGD and CBIA methods in improving the correctness of children’s fever self medication.