Sri Widawati
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia, Jln. Raya Jakarta-Bogor Km. 46, Cibinong Science Center, Jawa Barat, Indonesia

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PENGUJIAN PUPUK HAYATI KALBAR UNTUK MENINGKATKAN PRODUKTIVITAS TANAMAN KEDELAI (Glycine max) var. BALURAN Subowo, Yohanes Bernard; Sugiharto, Arwan; Suliasih, Suliasih; Widawati, Sri
Caraka Tani: Journal of Sustainable Agriculture Vol 25, No 1 (2010): March
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.022 KB) | DOI: 10.20961/carakatani.v25i1.15756

Abstract

Research aimed to evaluate the ability of kalbar biofertilizer to improve productivity of soybean. Experiment was conducted in the field experimental plot of Cibinong Science Centre. Soybean (Glycine max) var Baluran was treated with a series of fertilizer i.e.: compost containing Nitrogen fixing microbes, compost containing Phosphate solubilizing microbes, compost containing lignocelullosic degrading fungus, Kalbar biofertilizer (compost containing Nitrogen fixing, Phosphate solubilizing and lignocellulosic degrading microbes), chemist fertilizer, compost and control. The height of plant, fresh weight of biomass, number of leaves, pod number, and weight of seed were observed. The Nitrogen, Phosphate and Carbon which exist on the soil observed before planting, while flowering and while harvesting. The result was the application of kalbar biofertilizer able to increase the Carbon (C), Nitrogen (N) and Phosphor (P) content in the soil. This treatment also increased the fresh weight of biomass (22%), the number of pods (11, 11%) and the weight of seed (12,22%).
PENGUJIAN PUPUK HAYATI KALBAR UNTUK MENINGKATKAN PRODUKTIVITAS TANAMAN KEDELAI (Glycine max) var. BALURAN Subowo, YB.; Sugiharto, Arwan; Suliasih, Suliasih; Widawati, Sri
Caraka Tani - Jurnal Ilmu Ilmu Pertanian Vol 25, No 1 (2010)
Publisher : Caraka Tani - Jurnal Ilmu Ilmu Pertanian

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Abstract

Penelitian     mengarah     pada     evaluasi     kemampuan     dari      pupuk     hayati     kalbar     untuk meningkatkan   produktivitas  dari  tanaman  kedelai.  Percobaan  pada  plot  percobaan  lapangan  di  LIPI Cibinong.  Kacang  kedelai(  Glycine  max )   variasi  Baluran  diperlakukan  dengan  satu rangkaian  pupuk yaitu: Nitrogen berisi kompos mikroba perbaikan, Fosfat berisi kompos mikroba solubilizing, kompos mengandung lignocelullosic menurunkan populasi jamur, Pupuk hayati Kalbar (Kompos memperbaiki kandungan  nitrogem, Fosfat solubilizing  dan lignocellulosic  mengurangi  derajat mikroba), pupuk kimia, kompos  dan  kontrol.  Kenaikan  pupuk    berat  segar  dari  biomass,  angka  dari  daun-daun,  angka  buah polong, dan berat dari biji diamati. Nitrogen, Fosfat dan Karbon yang berada pada tanah sebelum tanaman, saat   fase  generatif   pembungaan   dan   saat   panen.   Hasil   aplikasi   pengujian   pupuk   kalbar   mampu meningkatkan  Karbon  (C  ), Nitrogen  (N  ) dan  Phosphor  (P  ) konten  pada  tanah.  Perlakuan  ini  juga meningkatkan berat segar dari biomass (22%), buah polong (11, 11%) dan berat dari biji (12,22%).
Uji Bakteri Simbiotik dan Nonsimbiotik Pelarutan Ca vs. P dan Efek Inokulasi Bakteri pada Anakan Turi (Sesbania grandiflora L. Pers.) Widawati, Sri
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1272.297 KB) | DOI: 10.14203/jbi.v11i2.2204

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ABSTRACTNitrogen-fixing bacteria that dissolve phosphate and produce IAA (plant growth hormones) are useful for biological organic fertilizer (BOF). Thirty nitrogen-fixing isolates have been isolated from the rhizosphere soil on Mount Salak, Bogor. This study aims to find nitrogen-fixing symbiotic and non-symbiotic bacteria that could potentially dissolve phosphorus (P) and produce hormones IAA. The N-fixing bacteria were tested to promote growth of turi (Sesbania grandiflora) during  seedling. Turi is called astringency tree, because the entire tree is useful for land reclamation, produce tannin, gum, resin, vegetables, medicine, tonic, antipyretic, and as pulp material. Pikovskaya medium was used to evaluate the ability of nitrogen-fixing bacteria to dissolve phosphate. The effect of bacteria on the growth of seedlings Sesbania grandiflora were evaluated using pot experiment. Pot contained sterile sand were arranged as factorial with 3 replications and watered with sterile distilled water containing Muller solution in the green house. The results showed that, the highest populations of bacteria, P solubilization index, and PMEase produced by Rhizobium, Azotobacter and Azospirillum were isolated from the rhizosphere of tea plant. The isolates were identified as Azotobacter chrococcum and Azospirillum lipoferum and Rhizobium sp. All of the isolates produced plant growth hormon (IAA). Effects of inoculants ABC (Azospirillum, Azotobacter, Rhizobium 1), ABD (Azospirillum, Azotobacter, Rhizobium 2), BCD (Azotobacter, Rhizobium 1, Rhizobium 2), dan ABCD (Azospirillum, Azotobacter, Rhizobium 1, Rhizobium 2), were very good on growth of Turi plant seedlings. All isolates formed root nodules, except the ABD and D isolats. Keywords: Nitrogen fixing bacteria, Phosphate solubilizing bacteria, Sesbania grandiflora L.Pers. 
AKTIVITAS ENZIM PELARUT FOSFAT DAN EFEKTIVITAS MIKROBA ASAL WAMENA UNTUK MENUNJANG PERTANIAN RAMAH LINGKUNGAN PADA DAERAH MARGINAL Widawati, Sri
Jurnal Teknologi Lingkungan Vol. 11 No. 3 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (776.933 KB) | DOI: 10.29122/jtl.v11i3.1194

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The activity of phosphate solubilizing enzyme and the effectivity of microbe from Wamena for support agriculture of environmental friendliness at marginal area. Phosphate dissolution of enzyme by microbial effective in compost plus from Wamena forest was stored in the freezer for 4 years, have been conducted in Ecofisiology laboratory,Microbiology devision, Research Center for Biology, Indonesian Institute of Science. This research was conducted to know the microbial affectivity and the activity of phosphate solubilizing enzyme of 20 microorganisms in inoculants stored 4 years in refrigerated room and to support soil fertility and biofertilizer agent in organic farming system. This research used plate count methode for counting the amount of microbial population, Scinner method was used toanalizer enzyme of alkaline and acid PME-ase,and statistical analysis use Duncan methode. The storage of 20 inoculants invected 20microorganisms for 4 years in refrigerated room showed that the microbial activity still high with the amount of mean population 107 and the activity of phosphate solubilizing enzyme with mean diameter of clear zone (holozone) 1 cm – 2 cm. The effectivity and the highest activity of phosphate solubilizing enzyme were : Azotobacter indicus (A), Bacillus panthothenticus (D), Bacillus megaterium (M), Bacillus thuringiensis (O), Ceratia sp. (R),Chromobacterium lividum (G), Chromobacterium violaceum (S), Flavobacterium breve(T), Klebsiella aerogenes (H), Pseudomonas fluorescent (J), Rhizobium legurxinosarium (L), and the lowest were: Streptomyces sp. (I) .Key word: Alkaline and acid PMEase, Phosphate solubilizing bacteria,Biofertolizer
Pengaruh Inokulasi Bakteri Terhadap Pertumbuhan Awal Jarak Pagar (Jatropha curcas L.) Widawati, Sri; Rahmansyah, Maman
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3175

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ABSTRACTBacterial inoculants affect the early growth of Jatropha (Jatropha curcas L). Genera ofAzotobacter, Bacillus, Chromobacterium, Citrobacter, Nitrosomonas, Rhizobium, andSpaerotillus natans were soil bacterial isolates. The soil was collected from numerous placesaround Pontianak, West Kalimantan. Those isolates were used as inoculants, and formulatedto single and mixed bacterial inoculants, then used to stimulate the early growth of jatrophaseedling in 15 weeks at greenhouse condition. Bacterial inoculations caused better growthperformance compared to its control as pure soil garden medium without inoculations, andneither to bare soil dresses with compost. In the presence of inoculants, plant height wasaccelerated quickly while other inoculants affected to stalk diameter development. Daily growthperformance of jatropha peaked in 8 and 11 weeks after inoculation of Citrobacter andNitrosomonas bacterial component were used as single inoculant, respectively. The increasingof shoot biomass accumulation was three times as caused by single inoculants (Bacillus sp),and the highest one up to four times of biomass weight caused by a mixture inoculants asconsortium of Azotobacter, Bacillus, and Nitrosomonas spp. That selective inoculant hasopportunity to be used for jatropha farming, and this basic study is meaningful to jatropacultivation for standing to bio-fuel resources.Keywords: Jatropha curcas L., inoculants, Azotobacter, Bacillus, Chromobacterium,Citrobacter, Nitrosomonas, Rhizobium , Spaerotillus natans.Kata kunci: Jatropha curcas L., inoculants, Azotobacter, Bacillus, Chromobacterium,Citrobacter, Nitrosomonas, Rhizobium , Spaerotillus natans.
ISOLASI DAN AKTIVITAS PLANT GROWTH PROMOTING RHIZOBACTERIA (RHIZOBIUM, AZOSPIRILLUM, AZOTOBACTER, PSEUDOMONAS) DARI TANAH PERKEBUNAN KARET, LAMPUNG [Isolation And Activity Of Plant Growth Promoting Rhizobacteria (Rhizobium, Azospirillum, Azotobacter, Pseudomonas) From Soil Of Rubber Plantation, Lampung] Widawati, Sri
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (840.976 KB) | DOI: 10.14203/beritabiologi.v14i1.1868

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Analysis of phosphate dissolving activity, P dissolved; PMEase and IAA production by Plant Growth Promoting Rhizobacteria group(PGPR)is a parameter to determine the effectiveness of these bacteria as biological organic fertilizer (BOF).This study was aimed to obtain PGPR (Rhizobium, Azospirillum, Azotobacter, Pseudomonas) that were potential as a BOF to reclamation on degraded plantation area. This study used a random sampling method for 11 sampling points (1999P1, 1999P2, 1999P3, 1999P4, 1999P5, 2007P, 2007P1,2007P2, 2007P3, 2007P4, 2007P5) in the area of rubber plant roots.Isolation and counting of bacterial populations used plate count method on selective media (YEMA, Okon, Caceres, NFB AMA, PAB) whereas phosphate dissolving activity analysis, Dissolution Efficiency Index (DE), PMEase and IAA production following the method of Nguyen, Bray, Seshadri, Tabatabai, and Gravel.The results showed that of the 11 sampling points, 11 isolates were obtained effectively consisting of four groups of bacteria, namely: Rhizobium,5 5 5 5 Azospirillum, Azotobacter and Pseudomonas with the number of population: 3.3x10 , 80x10 , 20x10 , and 14x10 cfu.The results of the analysis of DE, soluble P, PMEase acid-base, and the highest IAA obtained from the bacterium isolated from the sampling point 1999P3 and 1999P1. This potential isolates will be used to the next research on reclamation of degraded land plantation
Pengaruh Introduksi Kompos Plus terhadap Produkasi Bobot Kering Daun Kumis Kucing (Orthosiphon aristatus) pada Tiga Macam Media Tanah Widawati, Sri; Suliasih, Suliasih; Syaifudin, Syaifudin
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.723 KB) | DOI: 10.14203/jbi.v3i3.3475

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ABSTRACTA green house experiment were conducted to study the application of “compost plus” on the growth of Orthosiphon aristatus. The experiment was designed in Complete Randomized Design with factorial and five replicates. The first factors were fertilizer application (compost plus, compost, and control). The second factors were 3 kinds of soil (i.e. soil from Cibinong, Ciomas, and Sukabumi). The result showed that compost plus application increased 113.90 gram/pot of dry weight of Orthosispon aristatus leaves compared with compost application and control in 3 kinds of soil.Key words : Compost plus (compost + microbes), Orthosispon aristatus
Pengaruh Kompos dan Berbagai Pupuk Hayati terhadap Pertumbuhan dan Hasil Temulawak (Curcuma xanthorrhiza) Sugiharto, A.; Widawati, Sri
JURNAL BIOLOGI INDONESIA Vol 3, No 9 (2005): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i9.3280

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ABSTRACTThe Influence of Compos and Several Biofertilizers on the Growth and the Harvesting ofTemulawak (Curcuma xanthorrhiza). Curcuma xanthorriza is one of the potentialmedicinal plants. Usually this plant is planted only as additional plant among the mainhorticultural plants. This plant is used as medicine plant, and usually grown not in fertile soils.So, this research tried to use the additional fertilizer such as compost and the potentialmicrobes. The aim of the research was to know the influence of the compost and the potentialisolates on the growth of the Curcuma xanthorrhiza. The research had been done at the greenhouse with Randomized Completely Design, by using polybag with 20 cm diameter. Therewas three replications. The results analyzed with ANOVA method, and Duncan Test at 0,05.The used soil was podsolik yellowish red that collected from Darmaga, Bogor. The resultshowed that the use of compost and biofertilizer increased the harvesting of the tubers from15,76% to 52,79%. The using of either BPP isolates or the mycorrhyza, increased the driedweigh of tubers of Curcuma xanthorrhiza.Keywords : Compost, potential microbes, Curcunla xanthorrhiza
PENGARUH SALINITAS DAN INOKULAN BAKTERI TERHADAP PERTUMBUHAN TANAMAN TERUNG (Solanum melongena L.) [The Effect of Salinity and Bacteria Inoculant on The Growth of Eggplant (Solanum melongena L.)] Suliasih, Suliasih; Widawati, Sri
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3403.499 KB) | DOI: 10.14203/beritabiologi.v15i1.2854

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Nitrogen fixing dan phosphate solubilizing bacteria are important PGPR bacterial in addition of plant nutrients by increasing N and P, especially in saline soils. Experiments were conducted to observe the effects of  soil salinity and bacterial inoculant on growth eggplant (Solanum melongena) at green house of  Microbiology division, Research Center for Biology, The Indonesian Institute of Sciences,  Cibinong. Experiment laid out as factorial based randomized complete design  with three replications. Five levels of watering consisted of (1). Fresh water, (2). NaCl 0.1%, (3). NaCl 1%, (4). Sea water 75 %+25% fresh water, (5). Sea water 100%  as first factors. Five levels of inoculations/biofertilizers consisted of (1).control without fertilizer (K), (2). NPK (P), (3). Nitrogen Fixing bacteria (BPN1), all treatments was repeated 3 times  BPN2, mixed inoculation (BPN1 +  BPN2 + Phosphate Solubilizing Bacteria/BPF) as second factors.  Results showed that biofertilizer application increased growth and yield of eggplant in various levels of salinity at about 21.56%-53.35%  compared to uninoculated plants
THE POPULATION OF NITROGEN FIXING BACTERIA AND PHOSPHATE SOLUBILIZING BACTERIA IN THE RHIZOSPHERE FROM GUNUNG HALIMUN NATIONAL PARK Widawati, Sri; Suliasih, Suliasih
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.562 KB) | DOI: 10.14203/beritabiologi.v5i6.1075

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The biodiversity of nitrogen fixing bacteria (Rhizobium, Azotobacter and Azospirillum) and phosphate solubilizing bacteria in rhizosphere collected from Gunung Halimun National Park were studied.Bacterial population was counted from soil rhizosphere collected from various ecosystem types (determined based on elevation) namely 600 m, 1000 m, 1100 m, 1500 m and 1800 m above sea level.The results showed that the highest population of N-fixing and P solubilizing bacteria at Cikaniki (1100 asl) are founded in the plant rhizosphere of Schima wallichii in plot Suzuki A I and Altingia exelsa in Suzuki A III,at Gunung Botol area is in 1000 m asl,and Ciptarasa area is in 600 m asl.The population of N-fixing and P solubilizing bacteria at Gunung Halimun was influenced by the vegetation type, soil pH,and the elevation of area. Rhizobium, Azotobacter, Azospirillum (N-fixing bacteria); Pseudomonas sp, and Bacillus megaterium (P solubilizing bacteria), are most dominant at 600 m, 1000 m and 1100 m asl.