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All Journal Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
Apriliani Devinta
Sepuluh Nopember Institute of Technology
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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VIABILITY AND PRODUCTION CALCIFYING BACTERIAL ENDOSPORE ON SAND-CEMENT CARRIER Apriliani Devinta; Enny Zulaika
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.184

Abstract

Carbonatogenic bacteria have the ability to precipitate calcium carbonate (CaCO3) and many calcareous areas are found. Some of its species formed endosporas which resistant to harsh physical condition such as very alkaline pH. The objectives of this study were to obtain spore biomass and determine endosporas viability in tested carrier media such as sand-cement. The tested isolates were Bacillus JA1, JB3, SU1, AK4, Lysinibacillus JB2, and Sporosracina JA4. The production of endosporas was carried out on yeast urea broth with a temperature treatment of 70°C for 20 minutes. The formed endosporas were stored in carrier medium of sand, cement, and a mixture of cement sand. Spore viability was conducted using total plate count method. The results showed that all isolates were able to produce endosporas with the highest endosporas dry biomass is Bacillus AK4 which was 196 mg/L. After 2 hours of storage, endosporas were still able to grow by forming colonies on nutrient agar media.
Co-Authors Enny Zulaika